Role of the two PsaE isoforms on O2 reduction at photosystem I in Arabidopsis thaliana

Krieger‐Liszkay, Anja; Shimakawa, Ginga; Sétif, Pièrre

doi:10.1016/j.bbabio.2019.148089

Cited by 16 publications

(10 citation statements)

References 30 publications

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“…The expression of this gene was downregulated in shoots of the Eryoma cultivar and was upregulated in roots (Table 2). A. thaliana mutants deficient in PsaE1 had stunted growth, was pale, suffered from enhanced PSII photoinhibition, and showed a lower level of P700 oxidation under steady-state conditions [24]. In the Eryoma cultivar, we also found a decrease in shoot expression of the CB121 homologue encoding chlorophyll a-b binding protein 1B-21.…”

Section: Gene Expression In γ-Stimulated Cultivarsmentioning

confidence: 66%

“…Reactive oxygen species (ROS) are produced by plants during normal cell metabolism, and their production is significantly increased under stress conditions [24]. ROS signals work upstream and downstream from many other second messengers, and these molecules can directly or indirectly regulate expression of many genes, including those related to growth and development [25].…”

Section: Gene Expression In γ-Stimulated Cultivarsmentioning

confidence: 99%

“…Radiation exposure provokes overproduction of ROS [8], and this can change the redox state of chloroplasts [26] and eventually influence photosynthetic processes [5]. The PSAE homologue encodes photosystem I (PSI) reaction center subunit IV, which stabilizes the interaction between PSI subunits and takes part in ferredoxin metabolism [24]. The expression of this gene was downregulated in shoots of the Eryoma cultivar and was upregulated in roots (Table 2).…”

Section: Gene Expression In γ-Stimulated Cultivarsmentioning

confidence: 99%

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Studying Gene Expression in Irradiated Barley Cultivars: PM19L-like and CML31-like Expression as Possible Determinants of Radiation Hormesis Effect

et al. 2020

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Gamma (γ)-irradiation of plants at low doses can provoke a broad range of growth-stimulating effects. In order to reveal universal target genes that are involved in molecular pathways of radiation hormesis establishment, we studied nine barley cultivars for their tolerance to γ-irradiation of seeds. Four morphological traits were assessed in barley seedlings after γ-irradiation of seeds at 20 Gy. Nine cultivars were sorted according to the sensitivity to irradiation as γ-stimulated, “no morphological effect”, or γ-inhibited. Gene expression of 17 candidate genes was evaluated for the 7 most contrasting cultivars. Changes in expression of barley homologues of PM19L and CML31 were suggested as possible determinants of radiation hormesis effect. The possible role of jasmonate signaling in roots in radiation growth stimulations was revealed. Morphological analysis and gene expression study showed that the genetic background of a cultivar plays an important role in eustress responses to low-dose γ-irradiation of seeds.

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Section: Gene Expression In γ-Stimulated Cultivarsmentioning

confidence: 66%

Section: Gene Expression In γ-Stimulated Cultivarsmentioning

confidence: 99%

Section: Gene Expression In γ-Stimulated Cultivarsmentioning

confidence: 99%

See 1 more Smart Citation

Studying Gene Expression in Irradiated Barley Cultivars: PM19L-like and CML31-like Expression as Possible Determinants of Radiation Hormesis Effect

et al. 2020

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“…Especially mutants with slight defects in the turnover of the cytochrome b6f complex or in photosystem I may be of interest since their PQ pool is in a more reduced state. Mutants lacking small subunits, for example PsaI (Schöttler et al, 2017), or expressing only one isoform of certain subunits, for example PsaE1 or PsaE2 (Hald et al, 2008;Krieger-Liszkay et al, 2020), which do not show a strong phenotype in control conditions seem to be ideal candidates to test their resistance to drought stress. A: 4-week-old A. thaliana wild type and nsi1, nsi2, stn7 plants well-watered and after 6 days without watering.…”

Section: Discussionmentioning

confidence: 99%

Increased drought resistance in state transition mutants is linked to modified plastoquinone pool redox state

Leverne

Roach

Perreau

et al. 2023

Preprint

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Identifying traits that exhibit improved drought resistance is highly important to cope with the challenges of predicted climate change. We investigated the response of state transition mutants to drought. Compared with the wild type, state transition mutants were less affected by drought. Photosynthetic parameters in leaves probed by chlorophyll fluorescence confirmed that mutants possess a more reduced plastoquinone (PQ) pool, as expected due to the absence of state transitions. Seedlings of the mutants showed an enhanced growth of the primary root and more lateral root formation. The photosystem II inhibitor 3-(3,4-dichlorophenyl)-1,1-dimethylurea, leading to an oxidized PQ pool, inhibited primary root growth in wild type and mutants, while the cytochrome b6f complex inhibitor 2,5-dibromo-3-methyl-6-isopropylbenzoquinone, leading to a reduced PQ pool, stimulated root growth. A more reduced state of the PQ pool was associated with a slight but significant increase in singlet oxygen production. Singlet oxygen may trigger a, yet unknown, signaling cascade promoting root growth. We propose that photosynthetic mutants with a deregulated ratio of photosystem II to photosystem I activity can provide a novel path for improving crop drought resistance.

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“…Accordingly, O 2 reduction is favored when the disulfide bridge in PsaF is reduced while Fd reduction becomes less efficient. The subunit PsaE seems to be crucial for O 2 reduction at PSI in higher plants (Krieger-Liszkay et al, 2020). Unfortunately, we were not able to detect significant differences in the reduction level of PsaF in the two light regimes and in the mutant lines analyzed where distinct O 2 reduction levels were clearly found (Fig.…”

Section: Discussionmentioning

confidence: 99%

A complex and dynamic redox network regulating oxygen reduction at photosystem I

Hani,

Naranjo,

Shimakawa

et al. 2023

Preprint

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Thiol-dependent redox regulations of enzyme activities play a central role in regulating photosynthesis. Beside the regulation of metabolic pathways, alternative electron transport has been shown to be subjected to thiol-dependent regulation. We investigated the regulation of O2reduction at photosystem I. The level of O2reduction in leaves and isolated thylakoid membranes depends on the photoperiod in which plants are grown. We used a set of Arabidopsis mutant plants affected in the stromal, membrane and lumenal thiol network to study the redox protein partners involved in regulating O2reduction. Light-dependent O2reduction was determined in leaves and in thylakoids of plants grown in short day and long day conditions using a spin-trapping EPR assay. In wild type samples from short day, ROS generation was twice the amount of that in samples from long day, while this difference was abolished in several redoxin mutants. An in vitro reconstitution assays showed that thioredoxin m, NADPH-dependent reductase C (NTRC) and NADPH are required for high O2reduction levels in long day thylakoids. Using isolated photosystem I, we also show that reduction of a PSI protein is responsible for the increase in O2reduction. Furthermore, differences in the membrane localization of thioredoxins m and 2-Cys peroxiredoxin were demonstrated between thylakoids of short day and long day plants. Finally, we propose a model of redox regulation of O2reduction according to the reduction power of the stroma and the capabilities of the different thiol-containing proteins to form a network of redox interactions.

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Role of the two PsaE isoforms on O2 reduction at photosystem I in Arabidopsis thaliana

Abstract: High lights-Plants grown in short day generate more ROS at photosystem I than in long day-The subunit PsaE2 of photosystem I facilitates the reduction of oxygen

Cited by 16 publications

References 30 publications

Studying Gene Expression in Irradiated Barley Cultivars: PM19L-like and CML31-like Expression as Possible Determinants of Radiation Hormesis Effect

Studying Gene Expression in Irradiated Barley Cultivars: PM19L-like and CML31-like Expression as Possible Determinants of Radiation Hormesis Effect

Increased drought resistance in state transition mutants is linked to modified plastoquinone pool redox state

A complex and dynamic redox network regulating oxygen reduction at photosystem I

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