Role of the universal stress protein UspA of Salmonella in growth arrest, stress and virulence

Liu, Wen-Tssann; Karavolos, Michail H.; Bulmer, David M.; Allaoui, Abdelmounaaïm; Hormaeche, Raquel Demarco Carlos E.; Lee, Jeong Jin; Khan, C. M. Anjam

doi:10.1016/j.micpath.2006.09.002

Cited by 88 publications

(82 citation statements)

References 32 publications

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“…uspBA encodes the universal stress proteins A and B. The expression of the UspA stress protein is affected by metabolic, temperature, and oxidative stresses (38). Inactivation of the uspA gene in S. enterica serotype Typhimurium leads to decreased survival in stress conditions (38).…”

Section: Resultsmentioning

confidence: 99%

Salmonella enterica Serovar Enteritidis Genes Induced during Oviduct Colonization and Egg Contamination in Laying Hens

Gantois

Ducatelle

Pasmans

et al. 2008

Appl Environ Microbiol

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Salmonella enterica serovar Enteritidis is the predominant serovar associated with salmonellosis worldwide, which is in part due to its ability to contaminate the internal contents of the hen's egg. It has been shown that S. enterica serovar Enteritidis has an unusual tropism for the avian reproductive tract and an ability to persist in the oviduct and ovary. Factors allowing S. enterica serovar Enteritidis strains to contaminate eggs could be a specific interaction with the oviduct tissue, leading to persisting oviduct colonization. In vivo expression technology, a promoter-trap strategy, was used to identify genes expressed during oviduct colonization and egg contamination with S. enterica serovar Enteritidis. A total of 25 clones with in vivo-induced promoters were isolated from the oviduct tissue and from laid eggs. Among the 25 clones, 7 were isolated from both the oviducts and the eggs. DNA sequencing of the cloned promoters revealed that genes involved in amino acid and nucleic acid metabolism, motility, cell wall integrity, and stress responses were highly expressed in the reproductive tract tissues of laying hens.

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Section: Resultsmentioning

confidence: 99%

Salmonella enterica Serovar Enteritidis Genes Induced during Oviduct Colonization and Egg Contamination in Laying Hens

Gantois

Ducatelle

Pasmans

et al. 2008

Appl Environ Microbiol

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“…UspA of Salmonella spp. was reported previously to be important for virulence in mice and survival in host cells (29). However, usp1 and usp2 may not play important roles in the virulence of B. glumae, considering that Usp1 and Usp2 appear to play a role in overcoming heat shock stress but not other stresses.…”

Section: Discussionmentioning

confidence: 85%

Regulation of Universal Stress Protein Genes by Quorum Sensing and RpoS in Burkholderia glumae

Kim

Goo

Kang

et al. 2011

Journal of Bacteriology

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Burkholderia glumae possesses a quorum-sensing (QS) system mediated by N-octanoyl-homoserine lactone (C 8 -HSL) and its cognate receptor TofR. TofR/C 8 -HSL regulates the expression of a transcriptional regulator, qsmR. We identified one of the universal stress proteins (Usps), Usp2, from a genome-wide analysis of QS-dependent proteomes of B. glumae. In the whole genome of B. glumae BGR1, 11 usp genes (usp1 to usp11) were identified. Among the stress conditions tested, usp1 and usp2 mutants died 1 h after heat shock stress, whereas the other usp mutants and the wild-type strain survived for more than 3 h at 45°C. The expressions of all usp genes were positively regulated by QS, directly by QsmR. In addition, the expressions of usp1 and usp2 were dependent on RpoS in the stationary phase, as confirmed by the direct binding of RpoS-RNA holoenzyme to the promoter regions of the usp1 and usp2 genes. The expression of usp1 was upregulated upon a temperature shift from 37°C to either 28°C or 45°C, whereas the expression of usp2 was independent of temperature stress. This indicates that the regulation of usp1 and usp2 expression is different from what is known about Escherichia coli. Compared to the diverse roles of Usps in E. coli, Usps in B. glumae are dedicated to heat shock stress.

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“…Herein, we described the overexpression of two stress proteins: UspA and CspC. The role of the universal stress protein UspA in stress resistance was evaluated by Liu et al [34], who showed that inactivation of the uspA gene in S. Typhimurium leads to increased susceptibility to stress conditions. A retrospective study reported that the expression of UspA stress protein in E. coli was significantly affected by the expression of cspC [35].…”

Section: Discussionmentioning

confidence: 99%

Molecular analysis of the adaptive response in Salmonella Typhimurium after starvation in salty conditions

Elabed

Merghni

Hamza

et al. 2016

J Infect Dev Ctries

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Introduction: The pathogenic bacterium Salmonella enterica serovar Typhimurium elicits a variety of genetic programs to adapt to stress conditions encountered within hostile environments such as host phagocytes and preserved food. Methodology: In this work, differential display (DD) methodology was used to investigate the effect of one month starvation in a salty microcosm (0.5 M NaCl) on transcript profiling in a Salmonella Typhimurium LT2 strain. cDNA fragments resulting from differentially expressed mRNA were eluted from the gel, re-amplified, cloned, and then sequenced. Results: A total of 21 differentially expressed bands were detected by DD reverse transcription-polymerase chain reaction (RT-PCR). However, only 12 of them were successfully identified as upregulated genes in stressed cells. Based on the sequencing data and BLAST analysis, these genes were sopA, ssaD, yhhK, gmK, cspC, uspA, ompR, phoP, stcC, fimA, acrA,and yehZ. As a confirmation of the differential expression, RT-PCR was carried out using a set of specific primers. Remarkably, the expression levels of these genes were significantly increased in starved bacteria compared to standard laboratory conditions. Conclusions: Our results indicate that the starvation of Salmonella Typhimurium over one month in a salty microcosm changes the expression of stress proteins, response regulator in a two-component system, outer membrane proteins, effector proteins translocated by Salmonella pathogenicity island SPI1 and SPI2 type III secretion systems (TTSS), several metabolic enzymes, efflux pumps, and transport proteins. This suggests that the expression of the identified genes is important for the response of this pathogen to starvation in salt.

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Role of the universal stress protein UspA of Salmonella in growth arrest, stress and virulence

Cited by 88 publications

References 32 publications

Salmonella enterica Serovar Enteritidis Genes Induced during Oviduct Colonization and Egg Contamination in Laying Hens

Salmonella enterica Serovar Enteritidis Genes Induced during Oviduct Colonization and Egg Contamination in Laying Hens

Regulation of Universal Stress Protein Genes by Quorum Sensing and RpoS in Burkholderia glumae

Molecular analysis of the adaptive response in Salmonella Typhimurium after starvation in salty conditions

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