Role of the X and Y Chromosomes in the Female Germ Cell Line Development in the Mouse (Mus musculus)

Yamazaki, Wataru; Tan, Seang Lin; Taketo, Teruko

doi:10.1159/000521151

Cited by 2 publications

(2 citation statements)

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“…Another way to generate such ntESCs would be selective proliferation of XO populations from the existing male XY ntESCs. It is known that XO females in mice are fertile, unlike XO females in humans 35 . If we can proliferate XO female cells clonally to establish high-quality female ntESC lines, we will be able to restore wild-derived strains by natural mating of chimeric mice of both sexes.…”

Section: Discussionmentioning

confidence: 99%

Derivation of embryonic stem cells from wild-derived mouse strains by nuclear transfer using peripheral blood cells

Watanabe

Hirose

Hasegawa

et al. 2023

Sci Rep

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Wild-derived mouse strains have been extensively used in biomedical research because of the high level of inter-strain polymorphisms and phenotypic variations. However, they often show poor reproductive performance and are difficult to maintain by conventional in vitro fertilization and embryo transfer. In this study, we examined the technical feasibility of derivation of nuclear transfer embryonic stem cells (ntESCs) from wild-derived mouse strains for their safe genetic preservation. We used leukocytes collected from peripheral blood as nuclear donors without sacrificing them. We successfully established 24 ntESC lines from two wild-derived strains of CAST/Ei and CASP/1Nga (11 and 13 lines, respectively), both belonging to Mus musculus castaneus, a subspecies of laboratory mouse. Most (23/24) of these lines had normal karyotype, and all lines examined showed teratoma formation ability (4 lines) and pluripotent marker gene expression (8 lines). Two male lines examined (one from each strain) were proven to be competent to produce chimeric mice following injection into host embryos. By natural mating of these chimeric mice, the CAST/Ei male line was confirmed to have germline transmission ability. Our results demonstrate that inter-subspecific ntESCs derived from peripheral leukocytes could provide an alternative strategy for preserving invaluable genetic resources of wild-derived mouse strains.

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Section: Discussionmentioning

confidence: 99%

Derivation of embryonic stem cells from wild-derived mouse strains by nuclear transfer using peripheral blood cells

Watanabe

Hirose

Hasegawa

et al. 2023

Sci Rep

View full text Add to dashboard Cite

show abstract

“…More specifically, Yamazaki et al . compared transcriptomes among oocytes derived from XX, XO (i.e., monosomy X) and sex-reversed XY female mice ( Vaz et al, 2020 ; Yamazaki et al, 2022 ) along follicular growth up to maximum size allowed without significant morphological differences. They found losing a copy of X chromosome is the dominant effect on gene expression changes in only XO oocytes, whereas the transcriptome landscape in XY oocytes is associated with the expression of Y-linked genes.…”

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confidence: 99%