Role of Thermo-Sensitive Transient Receptor Potential Channels in Brown Adipose Tissue

Uchida, Kunitoshi; Sun, Wuping; Yamazaki, Jun; Tominaga, Makoto

doi:10.1248/bpb.b18-00063

Cited by 29 publications

(29 citation statements)

References 126 publications

(141 reference statements)

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“…The mechanism of cold-induced BAT activation is well known [67][68][69][70]. When the TRP channel in the skin receives a cold stimulus, the generated afferent nerve impulse is transmitted to the dorsal horn of the spinal cord and further to the hypothalamic preoptic area (POA), the thermoregulatory center.…”

Section: Discussionmentioning

confidence: 99%

“…Eventually, a nerve impulse from the excited SNS is transmitted to BAT via sympathetic preganglionic neurons (SPNs) located in the spinal intermediolateral nucleus (IML) of the spinal ventral horn (SVH). When released noradrenaline from the sympathetic nerve ends binds to β 3 adrenergic receptors on brown adipocyte membranes, uncoupling protein 1 (UCP-1) on the inner mitochondrial membrane is activated, and BAT thermogenesis is induced [67][68][69][70].…”

Section: Discussionmentioning

confidence: 99%

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Vigorous-Intensity Physical Activities Are Associated with High Brown Adipose Tissue Density in Humans

Tanaka

Fuse

Kuroiwa

et al. 2020

IJERPH

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Brown adipose tissue (BAT) plays a role in adaptive thermogenesis in response to cold environments and dietary intake via sympathetic nervous system (SNS) activation. It is unclear whether physical activity increases BAT density (BAT-d). Two-hundred ninety-eight participants (age: 41.2 ± 12.1 (mean ± standard deviation), height: 163.6 ± 8.3 cm, weight: 60.2 ± 11.0 kg, body mass index (BMI): 22.4 ± 3.0 kg/m2, body fat percentage: 25.4 ± 7.5%) without smoking habits were categorized based on their physical activity levels (a group performing physical activities including walking and moderate physical activity (WM) and a group performing WM + vigorous-intensity physical activities (VWM)). We measured the total hemoglobin concentration ([Total-Hb]) in the supraclavicular region, an index of BAT-d, and anthropometric parameters. [Total-Hb] was significantly higher in VWM than WM for all participant groups presumably owing to SNS activation during vigorous-intensity physical activities, and unrelated to the amount of total physical activity levels. Furthermore, multiple regression analysis revealed that BAT-d was related to visceral fat area and VWM in men and related to body fat percentage in women. We conclude that vigorous-intensity physical activities are associated with high BAT-d in humans, especially in men.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Vigorous-Intensity Physical Activities Are Associated with High Brown Adipose Tissue Density in Humans

Tanaka

Fuse

Kuroiwa

et al. 2020

IJERPH

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“…; Uchida et al . ). In addition, insulin receptors have been known to be expressed on DRG neurons (Sugimoto et al .…”

Section: Introductionmentioning

confidence: 97%

“…during skeletal muscle contraction) (Smith et al 2006;Burton et al 2016). Recent studies have demonstrated that insulin activates some ion channels reported as molecular candidates for mechanosensitive receptors in the peripheral terminals of primary sensory neurons for which the cell bodies are in the dorsal root ganglion (DRG) (Uchida & Tominaga, 2011;Colsoul et al 2013;Uchida et al 2018). In addition, insulin receptors have been known to be expressed on DRG neurons (Sugimoto et al 2002;Baiou et al 2007;Grote et al 2013;Lázár et al 2018) and peripheral nerves (Sugimoto et al 2002;Grote et al 2013).…”

Section: Introductionmentioning

confidence: 99%

Insulin potentiates the response to mechanical stimuli in small dorsal root ganglion neurons and thin fibre muscle afferents in vitro

Hotta

Katanosaka

Mizumura

et al. 2019

The Journal of Physiology

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Key points Insulin is known to activate the sympathetic nervous system centrally. A mechanical stimulus to tissues activates the sympathetic nervous system via thin fibre afferents. Evidence suggests that insulin modulates putative mechanosensitive channels in the dorsal root ganglion neurons of these afferents. In the present study, we report the novel finding that insulin augments the mechanical responsiveness of thin fibre afferents not only at dorsal root ganglion, but also at muscle tissue levels. Our data suggest that sympathoexcitation is mediated via the insulin‐induced mechanical sensitization peripherally. The present study proposes a novel physiological role of insulin in the regulation of mechanical sensitivity in somatosensory thin fibre afferents. Abstract Insulin activates the sympathetic nervous system, although the mechanism underlying insulin‐induced sympathoexcitation remains to be determined. A mechanical stimulus to tissues such as skin and/or skeletal muscle, no matter whether the stimulation is noxious or not, activates the sympathetic nervous system via thin fibre afferents. Evidence suggests that insulin modulates putative mechanosensitive channels in the dorsal root ganglion (DRG) neurons of these afferents. Accordingly, we investigated whether insulin augments whole‐cell current responses to mechanical stimuli in small DRG neurons of normal healthy mice. We performed whole‐cell patch clamp recordings using cultured DRG neurons and observed mechanically‐activated (MA) currents induced by mechanical stimuli applied to the cell surface. Local application of vehicle solution did not change MA currents or mechanical threshold in cultured DRG neurons. Insulin (500 mU mL−1) significantly augmented the amplitude of MA currents (P < 0.05) and decreased the mechanical threshold (P < 0.05). Importantly, pretreatment with the insulin receptor antagonist, GSK1838705, significantly suppressed the insulin‐induced potentiation of the mechanical response. We further examined the impact of insulin on thin fibre muscle afferent activity in response to mechanical stimuli in normal healthy rats in vitro. Using a muscle–nerve preparation, we recorded single group IV fibre activity to a ramp‐shaped mechanical stimulation. Insulin significantly decreased mechanical threshold (P < 0.05), although it did not significantly increase the response magnitude to the mechanical stimulus. In conclusion, these data suggest that insulin augments the mechanical responsiveness of small DRG neurons and potentially sensitizes group IV afferents to mechanical stimuli at the muscle tissue level, possibly contributing to insulin‐induced sympathoexcitation.

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“…The correlation between TRPs and energy metabolism has been extensively studied over the last few decades (Uchida et al 2011;Wang and Siemens 2015). Studies have reported that the intracellular concentration of Ca 2þ is relevant to thermogenesis (Uchida et al 2018). A series of ion channels are mainly involved in a plethora of Ca 2þ -mediated cell functions, whether the opening or closing of the TRPs could alter cell temperature still awaits to be explored.…”

Section: Introductionmentioning

confidence: 99%

Exploring the ‘cold/hot’ properties of traditional Chinese medicine by cell temperature measurement

Ding

et al. 2020

Pharmaceutical Biology

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Context: It is common sense that chewing a mint leaf can cause a cooling feeling, while chewing ginger root will produce a burning feeling. In Traditional Chinese Medicine (TCM), this phenomenon is referred to as 'cold/hot' properties of herbs. Herein, it is reported that TCM with different "cold/hot" properties have different effects on the variation of cells. Objective: To explore the intrinsic 'cold/hot' properties of TCM from the perspective of cellular and molecular biology. Materials and methods: A375 cells were selected using Cancer Cell Line Encyclopaedia (CCLE) analysis and western blots. Hypaconitine and baicalin were selected by structural similarity analysis from 56 and 140 compounds, respectively. A wireless thermometry system was used to measure cellular temperature change induced by different compounds. Alteration of intracellular calcium influx was investigated by means of calcium imaging. Results: The IC 50 values of GSK1016790A, HC067047, hypaconitine, and baicalin for A375 cells are 8.363 nM, 816.4 lM, 286.4 lM and 29.84 lM, respectively. And, 8 lM hypaconitine induced obvious calcium influx while 8 lM baicalin inhibited calcium influx induced by TRPV4 activation. Cellular temperature elevated significantly when treated with GSK1016790A or hypaconitine, while the results were reversed when cells were treated with HC067047 or baicalin. Discussion and conclusions: The changes in cellular temperature are speculated to be caused by the alteration of intracellular calcium influx mediated by TRPV4. In addition, the 'cold/hot' properties of compounds in TCM can be classified by using cellular temperature detection.

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Role of Thermo-Sensitive Transient Receptor Potential Channels in Brown Adipose Tissue

Cited by 29 publications

References 126 publications

Vigorous-Intensity Physical Activities Are Associated with High Brown Adipose Tissue Density in Humans

Vigorous-Intensity Physical Activities Are Associated with High Brown Adipose Tissue Density in Humans

Insulin potentiates the response to mechanical stimuli in small dorsal root ganglion neurons and thin fibre muscle afferents in vitro

Exploring the ‘cold/hot’ properties of traditional Chinese medicine by cell temperature measurement

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