Role of thyroid transcription factor-1 and P63 immunocytochemistry in cytologic typing of non-small cell lung carcinomas

Sinna, Eman Abu; Ezzat, Noha; Sherif, Ghada M.

doi:10.1016/j.jnci.2013.05.005

Cited by 8 publications

(8 citation statements)

References 44 publications

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“…The overall incidence of lung cancer was more common in males 31 cases (67.39%) as compared to females 15 cases (32.61%) with M:F ratio of 2.06:1. This ratio was comparable to study by Sinna EA et al 21 & Pavani et al 22 where sex ratio was 2.3:1.…”

Section: Discussionsupporting

confidence: 91%

Study of non-small cell lung cancer in cytology and correlation with clinicopathological parameters

Sharma¹,

Das²,

Panigrahi³

et al. 2021

IJPO

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Lung cancer is one of the most common causes of cancer related mortality. Various methods for cytological diagnosis like fine needle aspiration (FNA) along with exfoliative cytology by bronchial brushings play a pivotal role in the diagnosis as well as categorization of Non small cell lung carcinoma (NSCLC); especially in advanced stages and cases where biopsy may not be feasible. Aims & Objectives: The aim of the study was diagnosis and subclassification of Non small cell lung carcinoma (NSCLC) from cytosmears of bronchial brushings and Fine needle aspiration cytology (FNAC) samples followed by histopathological and clinicopathological correlation. Materials and Methods: The present study is both prospective & retrospective study done in Department of Pathology for a period of 2 years (September 2018-September 2020). A total of 46 cases were included in the study group. Categorization of lung cancer was done based upon the 2015 World Health Organization (WHO) criteria of cytodiagnosis. Histological correlation was done wherever available. Correlation with clinicopathological parameters was also done. Results: Total 46 patients were included in the study. The age range of was 32-81 years with a mean age of 59.2years. Male to female ratio was 2. 06:1. Smokers were affected in 26 cases (56.52%). The incidence of Adenocarcinoma (ADC) was commonest in our study comprising of 15 cases (32.61%). This was followed by squamous cell carcinoma (SCC) in 11 cases (23.91%), NSCLC-favours ADC in 8 cases (17.39%), NSCLC-NOS in 4 cases (8.70%), suspicious of malignancy in 3 cases (6.52%) and 2 cases (4.35%) each of NSCLC favours SCC & atypical and one case (2.17%) of ADC-lepidic pattern. Cytohistological correlation was possible in 25 core biopsy cases with 68% concordance. Conclusion: Cytological techniques are safer, economical and provide quick results. But a comprehensive clinicopathological approach is needed for labelling a correct diagnosis.

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Section: Discussionsupporting

confidence: 91%

Study of non-small cell lung cancer in cytology and correlation with clinicopathological parameters

Sharma¹,

Das²,

Panigrahi³

et al. 2021

IJPO

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“…A similar role can be played by a panel of four factors: p63, CK5/6, TTF1, and Napsin A [29]. IHC methods may be limited by the variability of the staining reactions caused by the heterogeneity of the tumor and some technical discrepancies, different sensitivities/specificities of individual markers, and the lack of standardization in the quantitative interpretation of the staining results [30]. Thus, there is an urgent need for the discovery of novel biomarkers to discriminate more precisely between SCC and AC.…”

Section: Discussionmentioning

confidence: 99%

Gene Expression Signature Differentiates Histology But Not Progression Status of Early-Stage NSCLC

Charkiewicz

Nikliński

Claesen

et al. 2017

Translational Oncology

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Advances in molecular analyses based on high-throughput technologies can contribute to a more accurate classification of non–small cell lung cancer (NSCLC), as well as a better prediction of both the disease course and the efficacy of targeted therapies. Here we set out to analyze whether global gene expression profiling performed in a group of early-stage NSCLC patients can contribute to classifying tumor subtypes and predicting the disease prognosis. Gene expression profiling was performed with the use of the microarray technology in a training set of 108 NSCLC samples. Subsequently, the recorded findings were validated further in an independent cohort of 44 samples. We demonstrated that the specific gene patterns differed significantly between lung adenocarcinoma (AC) and squamous cell lung carcinoma (SCC) samples. Furthermore, we developed and validated a novel 53-gene signature distinguishing SCC from AC with 93% accuracy. Evaluation of the classifier performance in the validation set showed that our predictor classified the AC patients with 100% sensitivity and 88% specificity. We revealed that gene expression patterns observed in the early stages of NSCLC may help elucidate the histological distinctions of tumors through identification of different gene-mediated biological processes involved in the pathogenesis of histologically distinct tumors. However, we showed here that the gene expression profiles did not provide additional value in predicting the progression status of the early-stage NSCLC. Nevertheless, the gene expression signature analysis enabled us to perform a reliable subclassification of NSCLC tumors, and it can therefore become a useful diagnostic tool for a more accurate selection of patients for targeted therapies.

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“…Previous studies have shown that on small specimens, only 47% to 78% is classifiable into specific subtype on morphology alone and unclassifiable (NSCC NOS) accounts for 22% to 53% cases. 8,22 Further subtyping is not possible on morphology alone due to absence of specific features or presence of overlapping features. 6,7 Therefore, to decrease NSCC-NOS cases, preparation of CB and application of ICC is required.…”

Section: Discussionmentioning

confidence: 99%

“…6,7 ICC as an adjunctive tool to cytomorphology is available in some studies in literature. 8,9 However, data using this on EBUS-TBNA material is limited with no study reported from India so far. [10][11][12][13] As cytology material may be conserved for molecular studies, a limited but optimal use of ICC needs to be determined.…”

Section: Introductionmentioning

confidence: 99%

Cytologic subtyping of lung carcinomas on Endobronchial ultrasound guided transbronchial needle aspiration (EBUS-TBNA) samples: Role of limited panel immunocytochemistry (ICC) as an adjunct to cytomorphology

Garg¹,

Bakshi²,

Verma³

2020

ACHR

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Majority of lung cancer presents at an advanced unresectable stage, emphasizing the diagnosis on cytological specimens like EBUS-TBNA. This study was aimed to subtype lung carcinomas on EBUS-TBNA specimens on smears and cell blocks (CB) and to assess the utility of limited panel ICC towards this subtyping. Materials and Methods: This is both retrospective and prospective study done on total of 142 cases for two and half years. The samples from either lung or mediastinal lymph nodes diagnosed as lung carcinoma on cytomorphology in correlation with clinico -radiological features were included. Extrapulmonary metastatic tumors, lymphomas and malignancies other than carcinomas were excluded. The cytologic material (EBUS-TBNA smears and CB) was obtained by EBUS TBNA procedure done on lung lesions and/or mediastinal lymph nodes and stained with required stains. All cases were first classified as small cell carcinoma (SCC) and non small cell carcinoma (NSCC) on cytomorphology as per 2004 WHO classification. NSCC was then further subtyped. ICC with TTF1/p63 antibodies was applied on NSCC and synaptophysin on SCC/neuroendocrine tumors. Napsin A was applied on p63/TTF1 negative cases. The results were expressed in frequencies and percentages. Result: On cytomorphology alone, 25 of 142 cases (17.6%) were classified as SCC and 117 cases (82.4%) as NSCC. NSCC was further subtyped on cytomorphology (smears+CB) into definitive (squamous cell carcinoma [SqCC]/adenocarcinoma [ADC]) v/s favouring (favouring SqCC/ ADC/ adenosquamous carcinoma) v/s not otherwise specified (NOS) in 41 (35.1%) v/s 33(28.2%) v/s 43(36.7%) cases respectively. Immunostains TTF1/ p63 and napsin A applied on possible 86 NSCC cases reduced NOS to 9.3 %. Synaptophysin confirmed all 20 cases of SCC and 1 large cell neuroendocrine carcinoma (LCNC). Overall in 142 cases, limited panel ICC reduced NSCC-NOS to 11.9%. Conclusion: Subtyping of lung carcinoma on EBUS-TBNA samples is feasible. Preparation of cell block allows application of ICC. A limited panel ICC comprising p63, TTF1 and synaptophysin can classify in majority of cases with napsin A useful as secondary antibody in p63, TTF1 negative cases. IntroductionThe aim of this study was to know the efficacy of cytomorphology (smears and CB) in subclassification of lung carcinomas on E BUS-TBNA samples & to assess the value of addition of limited panel ICC as an adjunct to cytomorphology in this subtyping.

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Role of thyroid transcription factor-1 and P63 immunocytochemistry in cytologic typing of non-small cell lung carcinomas

Abstract: TTF-1 achieved moderate sensitivity, and high specificity in the diagnosis of AC, while P63 was highly sensitive and specific for the diagnosis of SCC. Immunocytochemistry raised the sensitivity and specificity of FNAC in diagnosing AC and SCC using TTF-1 and P63, respectively.

Cited by 8 publications

References 44 publications

Study of non-small cell lung cancer in cytology and correlation with clinicopathological parameters

Study of non-small cell lung cancer in cytology and correlation with clinicopathological parameters

Gene Expression Signature Differentiates Histology But Not Progression Status of Early-Stage NSCLC

Cytologic subtyping of lung carcinomas on Endobronchial ultrasound guided transbronchial needle aspiration (EBUS-TBNA) samples: Role of limited panel immunocytochemistry (ICC) as an adjunct to cytomorphology

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