Role of Tissue Inhibitors of Matrix Metalloproteases-1 (TIMP-1) in Evolution of CCl4-Induced Liver Cirrhosis in Mice.

Abstract

Background Cirrhosis is a worldwide end stage liver disease. It is characterized by diffuse fibrosis and conversion of normal liver lobules into structurally abnormal nodules. Aim of the work This study was conducted to investigate the role of tissue inhibitors of matrix metalloproteases-1 (TIMP-1) in during the evolution of cirrhosis, and in fibers resolution after cessation of the insult. Materials and methods 54 adult male Balb/c mice; about 2 months old were randomly divided into control and treated groups;Control:24 animals.In the treated groups; 30 animals were subcutaneously injected with CCl4 (20% # diluted in sunflower oil) in a dose of 1 ml per Kg twice weekly.6 animals of them were sacrificed72 hours after the last dose at the 4th week, 8th week, 12th week, and 16th week.6 animals were kept for two weeks without injection after the 16 weeks of CCl4 treatment. Results Our results indicated that TIMP-1 is involved in the process of fibrogenesis and fibers resolution in an experimental model of cirrhosis in mice. Conclusion TIMP-1 is involved in the process of fibrogenesis in mice which can be applied in new strategies for the treatment of liver cirrhosis.