Role of toll like receptor-9 (1237 T/C) gene polymorphism in patients with type 2 diabetes and diabetic foot ulcer

Abstract

This study aimed to evaluate whether (1237 T/C) polymorphism is related with diabetic foot ulcer in type-2 diabetes mellitus of Iraqi patients in Najaf city. Diabetic foot ulcer (DFU) is one of the most prevalent and serious diabetic complications. DFU accounts for about 15% of the diabetic population. It is commonly associated with the increased diabetic morbidities and mortalities. The type 2 diabetes is recognized by aberrations of glucose, lipid and protein metabolism. It is increasingly appreciated that wound healing defect seen in diabetic patients is attributed to the altered protein and lipid metabolism. This study involved (180) subjects, between them, 60 were patients as group one suffering from type 2 diabetes and 60 were patient suffering from type 2 diabetes and foot ulcer as group two. In addition, 60 healthy group three as control subjects. Polymerase chain analysis was used for detection of TLR-9 genotypes followed by restriction analysis. We observed no significance differences in the distribution of the genotypes and alleles of (1237 T/C) polymorphism among the study groups.

Toll-like Receptor 9 Gene in the Development of Type 2 Diabetes Mellitus in the Saudi Arabian Population

“…However, a positive association was confirmed at the +1174 locus [29]. In the Iranian population, the rs352140 SNP was not associated with T2DM or DN [13]; however, this could be a result of small sample size. The rs352140 SNP was studied in Chinese children with T1DM, and a strong association was observed between the SNP and T1DM development [11].…”

“…The relationship between TLR9 and diabetes, specifically T2DM, is well established [12,13,17]. The rs352140 SNP was studied in T1DM, and it was confirmed that the rs352140 SNP was connected with residual β-cell function [11].…”

“…Effector molecules, such as cytokines and chemokines, regulate adaptive immune responses and are expressed when TLRs recognize pathogen-associated molecular patterns [12]. TLR9 is linked to autoimmune diseases and has been associated with highrisk asthma and low-risk diabetes [13]. TLR9 deficiency enhances pancreatic islet formation as well as β-cell differentiation and function [14].…”