In the present work we have used some of the most clinically important and tested techniques of spectroscopy to the study of Migraine. We have evaluated trace elements such as Ca, Zn, Mg, Fe ,Cu, Na and K in the present work .The results of the study are very helpful to the clinicians and scientists to maintain the levels of different types of trace elements . On the basis of trace elemental analysis we are in a position to reduce the higher levels of the certain trace elements in a particular level of recommendations by the world health organization. We have also applied the statistical analysis to check the feasibility and suitability of the data with the application of theory of statistics. We have calculated multiple partial correlation coefficient in seven elements altogether in migraine patients and compared the data with the control persons. The multiple correlations such asin migraine and control . The value of . R K ZnCuMgCaFeNa is found higher in the migraine patients in comparison to healthy persons .The other correlations were found to be lower in migraine patients .We must apply this study to maintain the levels of the trace elements according to correlation analysis .