Role of transient receptor potential channel 6 in the odontogenic differentiation of human dental pulp cells

Abstract

Pulp capping is a restorative technique employed in an attempt to maintain pulpal vitality and generate reparative dentin. Ca2+ released from capping materials is suggested to promote reparative dentin formation. Transient receptor potential channel 6 (TRPC6) is a receptor-operated Ca2+ channel that serves an important role in Ca2+ influx in the majority of non-excitable cells, and influences the calcium signaling and cell respond. Therefore, the purpose of the present study was to gain an insight into the role of TRPC6 in the odontoblastic differentiation of human dental pulp cells (HDPCs). Human dental pulp tissues and HDPCs were obtained from healthy third molars. By immunohistochemical staining, TRPC6 was observed to be highly expressed in the dental pulp tissue, particularly in the odontoblast layer. In addition, the protein level of TRPC6 was increased in a time-dependent manner during odontogenic differentiation of HDPCs. Downregulation of TRPC6 by a lentivirus vector containing TRPC6 shRNA inhibited the process of odontogenic differentiation in HDPCs. In conclusion, the current data demonstrated that TRPC6 served a significant role in the odontogenic differentiation of HDPCs, suggesting it may be a promising therapeutic target in regenerative endodontics.

Evaluation of odonto/osteogenic differentiation potential from different regions derived dental tissue stem cells and effect of 17β-estradiol on efficiency

“…Conditioned media from tooth germ and natural dentin matrix have been used for in vitro odonto/osteoblast differentiation from stem cells [ 6 , 8 , 9 ], however, it is difficult to maintain homogenous, constant conditions when using this media. In the present study, ODM contain various combinations of ingredients, including ß-glycerophosphate, ascorbate-2-phosphate, and dexamethasone, which makes odontoblast induction easier and produce constant results [ 1 , 23 ]. Recent studies revealed that estrogen is essential for in vivo and in vitro odontogenic and osteogenic differentiation of DPSCs [ 16 , 25 , 33 ].…”

“…Data are represented by the mean ± SD of four independent experiments. Lettered subscripts indicate statistical differences between groups (p < 0.05) of ingredients, including ß-glycerophosphate, ascorbate-2-phosphate, and dexamethasone, which makes odontoblast induction easier and produce constant results [1,23]. Recent studies revealed that estrogen is essential for in vivo and in vitro odontogenic and osteogenic differentiation of DPSCs [16,25,33].…”

“…Dental-derived MSCs were cultured in ADMEM supplemented with 10% FBS and differentiated at passage four into odonto/osteoblasts as previously described [ 1 , 22 , 23 ]. To investigate their capacity to differentiate into odonto/osteoblasts, all the four types of dental MSCs (at 70 ~ 80% confluence) were cultured in DMEM supplemented with 10% FBS, 1% penicillin/streptomycin, 10 mM ß-glycerophosphate, 50 µg/mL ascorbate-2-phosphate, 10 nM dexamethasone for 14 days.…”

The Role of Transient Receptor Potential (TRP) Channels in the Transduction of Dental Pain

“…TRPC1 and TRPC6 have been reported to be expressed in odontoblasts [179,197,198]. TRPC1 plays an important role in store-operated Ca 2+ entry (SOCE) in a variety of tissues and cell types [199,200].…”

“…TRPC6 protein expression increases with time during odontoblast differentiation from pulp tissue. Furthermore, differentiation is inhibited by downregulation of TRPC1 and TRPC6 expression, indicating involvement of these channels in the odontogenic differentiation of human dental pulp cells [197,198].…”