Role of trap lectins in the specificity of nematode capture by fungi

Rosenzweig, William D.; Premachandran, Devidas; Pramer, David

doi:10.1139/m85-131

Cited by 27 publications

(8 citation statements)

References 18 publications

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“…Some adhesive-secreting fungi entrap nematodes using lectins (Rosenzweig et al 1985). We also note that mRNAs encoding lectins were abundant in the adhesive slime gland of the onychophoran Euperipatoides rowelli (Haritos et al 2010), a terrestrial velvet worm that uses a jet of sticky quick-setting gel to immobilise its prey.…”

Section: Discussionmentioning

confidence: 72%

Identification of Proteins Associated with Adhesive Prints from Holothuria dofleinii Cuvierian Tubules

et al. 2014

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Cuvierian tubules are expelled as a defence mechanism against predators by various species within the family Holothuridae. When the tubules are expelled, they become sticky almost immediately and ensnare the predator. The mechanism of this rapid adhesion is not clear, but proteins on the surface of the expelled tubules are widely believed to be involved. This study has examined such proteins from Holothuria dofleinii, sourced from adhesive prints left on glass after the removal of adhered tubules. Gel electrophoresis showed that seven strongly staining protein bands were consistently present in all samples, with molecular masses ranging from 89 to 17 kDa. N-terminal sequence data was obtained from two bands, while others seemed blocked. Tandem mass spectrometry-based sequencing of tryptic peptides derived from individual protein bands indicated that the proteins were unlikely to be homopolymers. PCR primers designed using the peptide sequences enabled us to amplify, clone and sequence cDNA segments relating to four gel bands; for each, the predicted translation product contained other peptide sequences observed for that band that had not been used in primer design. Database searches using the peptide and cDNA-encoded sequences suggest that two of the seven proteins are novel and one is a C-type lectin, while-surprisingly-at least three of the other four are closely related to enzymes associated with the pentose phosphate cycle and glycolysis. We discuss precedents in which lectins and metabolic enzymes are involved in attachment and adhesion phenomena.

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Section: Discussionmentioning

confidence: 72%

Identification of Proteins Associated with Adhesive Prints from Holothuria dofleinii Cuvierian Tubules

et al. 2014

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“…The failure of JV-acetyl-D-galactosamine to inhibit capture and penetration may be due to strain differences between our fungal isolate and those used in previous studies or to the involvement of some other fungal lectin in the capture and recognition of T. colubriformis J3s. Rosenzweig, Premachandran & Pramer (1985) showed that despite the demonstration of lectins with specific carbohydrate binding properties on fungal traps, this specificity is not reflected in their ability to capture nematode prey.…”

Section: Discussionmentioning

confidence: 99%

Carbohydrate/lectin interactions between the nematophagous fungus,Arthrobotrys oligospora, and the infective juveniles ofTrichostrongylus colubriformis(Nematoda)

Wharton

Murray

1990

Parasitology

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Removal of the sheath of the ensheathed infective juvenile of Trichostrongylus colubriformis prevents capture by the nematophagous fungus Arthrobotrys oligospora. Exposure of the trap hyphae to a variety of saccharides, which may block a recognition system based on lectin/carbohydrate binding, failed to prevent capture but some saccharides did inhibit penetration and invasion by the fungus. Capture and penetration thus appear to be two distinct processes with capture being less specific than penetration. Carbohydrate residues were absent from the outer surface of the cuticle and the sheath but were present on the inner surface of the sheath. The limited accessibility of these lectin-binding sites may explain the slow process of infection of the infective juvenile by the fungus. The sheath does not protect the infective juvenile against attack by this nematophagous fungus.

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“…Different species of nematode-trapping fungi possess leetins of different specificities, suggesting specialization based on the surface glycoproteins of the prey. However, the actual rates of nematode capture do not match the biochemical expectations, leaving the question of specialization unresolved (Rosenzweig et al 1985).…”

Section: Discussionmentioning

confidence: 99%

Clonal Diversity in Populations of Polysphondylium Pallidum, A Cellular Slime Mold

Ketcham¹,

Eisenberg²

1989

Ecology

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Populations of the cellular slime mold Polysphondylium pallidum contain clones that differ in their abilities to use bacterial resources. We found abundant diversity among isolates obtained from plots of several square metres and from single soil cores 1.1 cm in diameter. The variation we were measuring was not due to genetic changes induced by our manipulation. Previously, isolation of different species of cellular slime molds from sample areas, metres to hectares in extent, has prompted studies of mechanisms of species coexistence in the face of assumed competition for bacterial resources. We conclude that competition has not only failed to eliminate different species from the community but has failed to eliminate clonal diversity from within species. Previous explanations of species coexistence need reevaluation.

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Role of trap lectins in the specificity of nematode capture by fungi

Cited by 27 publications

References 18 publications

Identification of Proteins Associated with Adhesive Prints from Holothuria dofleinii Cuvierian Tubules

Identification of Proteins Associated with Adhesive Prints from Holothuria dofleinii Cuvierian Tubules

Carbohydrate/lectin interactions between the nematophagous fungus,Arthrobotrys oligospora, and the infective juveniles ofTrichostrongylus colubriformis(Nematoda)

Clonal Diversity in Populations of Polysphondylium Pallidum, A Cellular Slime Mold

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