Role of triptolide in cell proliferation, cell cycle arrest, apoptosis and histone methylation in multiple myeloma U266 cells

Zhao, Fei; Chen, Yan; Linglan, Zeng; Li, Rui; Zeng, Rong; Wen, Lu; Liu, Yuan; Zhang, Chun

doi:10.1016/j.ejphar.2010.05.034

Cited by 45 publications

(38 citation statements)

References 37 publications

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“…[36][37][38][39][40] We observed that HNSCC cells surrounding the cancer nest had elevated Abbreviations: CI, confidence interval; EZH2, enhancer of zeste homolog 2; HR, hazard ratio; pN, pathologic lymph node status; TNM, tumor-lymph node-metastasis classification.…”

Section: Discussionmentioning

confidence: 96%

Up‐regulation of enhancer of zeste homolog 2 is associated positively with cyclin D1 overexpression and poor clinical outcome in head and neck squamous cell carcinoma

Cao

Feng

Cui

et al. 2011

Cancer

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BACKGROUND:The authors previously observed that enhancer of zeste homolog 2 (EZH2) overexpression was associated significantly with the development of oral cancer. In the current study, they investigated whether EZH2 can function as a prognostic predictor for patients with head and neck squamous cell carcinoma (HNSCC). METHODS: Expression levels of EZH2 in HNSCC cells were detected using reverse transcriptase-polymerase chain reaction (PCR) and Western blot analyses. In addition, the effects of EZH2 ablation on the proliferation and invasion of HNSCC cells were investigated through small interfering RNA (siRNA)-mediated knockdown. Real-time PCR and immunohistochemistry were used to evaluate EZH2 and cyclin D1 expression in 46 HNSCC samples, and the expression levels also were re-evaluated in 124 independent samples by immunohistochemistry. RESULTS: EZH2 expression was elevated remarkably in HNSCC specimens and cell lines. Upon EZH2 silencing, the proliferation and invasion of HNSCC cells were remarkably suppressed. EZH2 expression frequently was correlated with cyclin D1 expression (P ¼ .034) and tumor differentiation (P ¼ .020). In addition, both EZH2 messenger RNA levels and EZH2 protein levels were strongly associated with signs of histologic severity (P ¼ .012 and P ¼ .032, respectively). Univariate analysis revealed that high EZH2 expression was associated with worse overall survival (P ¼ .001) and disease-free survival (P ¼ .002). The combined expression of EZH2 and cyclin D1 had superior prognostic ability for patients with HNSCC than the expression of either marker alone. In multivariate analysis, EZH2 expression was identified as an independent predictor of overall and disease-free survival. CONCLUSIONS: The current results indicated that EZH2 is an independent prognostic indicator for patients with HNSCC. In addition, an analysis of the combined expression of EZH2 and cyclin D1 can serve as a more powerful prognostic predictor for patients with HNSCC. Cancer 2012;118:2858-71.

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Section: Discussionmentioning

confidence: 96%

Up‐regulation of enhancer of zeste homolog 2 is associated positively with cyclin D1 overexpression and poor clinical outcome in head and neck squamous cell carcinoma

Cao

Feng

Cui

et al. 2011

Cancer

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“…f. that has been used as a natural medicine in China for a long time, which was used for treating autoimmune diseases such as nephritis and rheumatoid arthritis Zhu et al, 2012;Huang et al, 2013;Tan et al, 2013). Newly report shown that triptolide have the potential to suppress the proliferation of human tumors in vitro (Zhu et al, 2009;Borja-Cacho et al, 2010;Clawson et al, 2010;Zhao et al, 2010;Lu et al, 2011). It is worth noting that triptolide possess side effects in both immunosuppressive and antifertility activities (Carter et al, 2008;Westfall et al, 2008;Yang et al, 2008;Chen et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

“…Another widely used drug, triptolide, the major composition extracted from the Chinese herb Tripteryglum wilfordii Hook.f, has now been shown that it can inhibit tumor growth (Clawson et al, 2010;Zhao et al, 2010;Lu et al, 2011;Li et al, 2012;Zhu et al, 2012;Huang et al, 2013;Tan et al, 2013). However, triptolide still have side effects such as immunosuppressive and antifertility (Carter et al, 2008;Westfall et al, 2008;Yang et al, 2008;Chen et al, 2009;Zhu et al, 2009;Borja-Cacho et al, 2010).…”

Section: Enhanced Anti-tumor Efficacy Of Aspirin Combined With Triptomentioning

confidence: 99%

Enhanced Anti-tumor Efficacy of Aspirin Combined with Triptolide in Cervical Cancer Cells

Chen

Yongjie²

2013

Asian Pacific Journal of Cancer Prevention

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“…As a step forward towards understanding the molecular details of substrate recognition by NSD-CDs, we used MD simulations to examine the binding characteristics of H3K4, H3K9, H3K27, H3K36, H3K79, and H4K20 peptides. Overall, our data indicate that all the histone H3 and H4 peptides tested (H3K4 a.a. 1-7; H3K9 a.a. 5-11; H3K27 a.a. 23-29; H3K36 a.a. 32-38; H3K79 a.a. 75-81; H4K20 a.a. [16][17][18][19][20][21][22] share similar steric hindrance and have similar positive charges related to their electric field and molecular surface. These properties imply favourable binding energies for the NSD-CDs (Table 1).…”

Section: Molecular Binding Details Of H3k4 H3k9 H3k27 H3k36 H3k79mentioning

confidence: 99%

“…The amplification of NSD1 has been reported in multiple myeloma, lung cancer, neuroblastoma and glioblastoma. The amplification of either NSD1 or NSD2 triggers cellular transformation [21][22][23][24][25][26][27][28]. NSD2 is associated with tumor aggressiveness or prognosis in most types of cancers, including prostate cancer and multiple myeloma and is overexpressed in at least 15 different cancers [9,10,17,18,20,21,25,[29][30][31][32][33].…”

Section: Introductionmentioning

confidence: 99%

In vitro histone lysine methylation by NSD1, NSD2/MMSET/WHSC1, and NSD3/WHSC1L

Morishita

Mevius

Luccio

2014

BMC Structural Biology

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Background: Histone lysine methylation has a pivotal role in regulating the chromatin. Histone modifiers, including histone methyl transferases (HMTases), have clear roles in human carcinogenesis but the extent of their functions and regulation are not well understood. The NSD family of HMTases comprised of three members (NSD1, NSD2/ MMSET/WHSC1, and NSD3/WHSC1L) are oncogenes aberrantly expressed in several cancers, suggesting their potential to serve as novel therapeutic targets. However, the substrate specificity of the NSDs and the molecular mechanism of histones H3 and H4 recognition and methylation have not yet been established.

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Role of triptolide in cell proliferation, cell cycle arrest, apoptosis and histone methylation in multiple myeloma U266 cells

Cited by 45 publications

References 37 publications

Up‐regulation of enhancer of zeste homolog 2 is associated positively with cyclin D1 overexpression and poor clinical outcome in head and neck squamous cell carcinoma

Up‐regulation of enhancer of zeste homolog 2 is associated positively with cyclin D1 overexpression and poor clinical outcome in head and neck squamous cell carcinoma

Enhanced Anti-tumor Efficacy of Aspirin Combined with Triptolide in Cervical Cancer Cells

In vitro histone lysine methylation by NSD1, NSD2/MMSET/WHSC1, and NSD3/WHSC1L

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