Role of Tumor Necrosis Factor-alpha and Interleukin-10 Gene Polymorphisms in Irritable Bowel Syndrome

Veek, Patrick P. J. van der; Berg, Marlies van den; Kroon, Yvette E de; Verspaget, Hein W.; Masclee, Ad

doi:10.1111/j.1572-0241.2005.00257.x

Cited by 183 publications

(148 citation statements)

References 45 publications

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“…Genomic DNA was isolated using the salting out method (Miller et al, 1988). In addition, DNA was extracted from peripheral blood leucocytes of 169 healthy volunteers (38% male, median age 33 years (range 18 -73 years), 495% Caucasian) as described before (van der Veek et al, 2005).…”

Section: Patients and Study Designmentioning

confidence: 99%

Clinical impact of MMP and TIMP gene polymorphisms in gastric cancer

et al. 2006

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Gastric cancers express enhanced levels of matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs). Single-nucleotide polymorphisms (SNPs) in MMP and TIMP genes may be associated with disease susceptibility and might also affect their antigen expression. We studied the genotype distribution and allele frequencies of SNPs of MMP-2, -7, -8 and -9 and TIMP-1 and -2 in gastric cancer patients in relation to tumour progression, patient survival and tissue antigen expression. The genotype distribution and allele frequencies were similar in gastric cancer patients and controls, except for MMP-7 À181A4G . In addition, the genotype distribution of MMP-7 À181A4G was associated with Helicobacter pylori status (w 2 7.8, P ¼ 0.005) and tumour-related survival of the patients. Single-nucleotide polymorphism TIMP-2 303C4T correlated significantly with the WHO classification (w 2 5.9, P ¼ 0.03) and also strongly with tumour-related survival (log rank 11.74, P ¼ 0.0006). Single-nucleotide polymorphisms of MMP-2, -8, -9 and TIMP-1 were not associated with tumour-related survival. Only the gene promoter MMP-2 À1306C4T polymorphism correlated significantly with the protein level within the tumours. First-order dendrogram cluster analysis combined with Cox analysis identified the MMP-7 À181A4G and TIMP-2 303C4T polymorphism combination to have a major impact on patients survival outcome. We conclude that MMP-related SNPs, especially MMP-7 À181A4G and TIMP-2 303C4T , may be helpful in identifying gastric cancer patients with a poor clinical outcome.

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Section: Patients and Study Designmentioning

confidence: 99%

Clinical impact of MMP and TIMP gene polymorphisms in gastric cancer

et al. 2006

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“…Besides the serotonin transporter gene, other studies have evaluated the 5-HT 2A receptor gene (5-HT2A, 5-hydroxytryptamine receptor receptor 2A) (21), the norepinephrine transporter gene (NET) (14), the alpha 2A -adrenergic receptor gene (ADRA2A) (14), alpha 2C -adrenergic receptor gene (ADRA2C) (14), as well as inflammatory genes including interleukin-10 (IL-10) (22,23), transforming growth factor-β1 (TGF-β) (22), tumor necrosis factor-alpha (TNF-α) (23), and the β3 subunit G protein (GNβ3) (24,25). Positive associations were observed between the AA or GA genotype of the −1082G/A polymorphism on the IL-10 gene and IBS, as well as the heterozygote GA genotype on the −308G/A polymorphism on the TNF-α gene.…”

Section: What Genes Have Been Evaluated In Ibs?mentioning

confidence: 99%

The Genetics of Irritable Bowel Syndrome

Saito

Petersen

Locke

et al. 2005

Clinical Gastroenterology and Hepatology

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Irritable bowel syndrome (IBS) is one of the most common diagnoses made by gastroenterologists and primary care providers alike, and yet the underlying mechanism remains poorly understood. Family and twin studies suggest that IBS may have a genetic basis. Several candidate gene association studies have been performed, but thus far, they have failed to clearly identify an "IBS gene." Epidemiological studies are needed to facilitate phenotype definition and identify relevant environment risk factors that will need to factor in gene and environment interactions in all future genetic studies. As genetic research in IBS is relatively nascent, much opportunity, as well as many challenges, exists in identifying the genes responsible for IBS.

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“…not duplicate these fi ndings in cultures of sigmoid biopsy samples from women with IBS and controls ( 15 ). Because cytokine expression is in part genetically determined, the imbalance in the pro-and anti-infl ammatory cytokines might be the consequence of polymorphisms, although at present, this remains unproven ( 13,16,17 ).…”

mentioning

confidence: 99%