In order to evaluate the effects of seed priming on morpho-physiological traits of winter wheat under drought stress, an experiment was conducted under semi-controlled conditions in the city of Ooshnaviyeh of Iran, during 2011-2012. This Study was carried out Factorial experiment based on RCBD design with 3 replications to determine the influence of Irrigation (normal and drought stress) and Priming control (with no priming), Ascrobat and hardening. Analysis of variance showed effect of irrigation on all characters except for 1000-grain weight and priming effect for all traits studied was significant. Also the interaction between priming and irrigation effects on Photosynthesis, Peduncle length, number grain per spike, number spike/m -2 was statistically significant. In this research drought significantly reduced all traits, as drought stress reduced grain yield 7.16% in comparison with control. Furthermore priming with Ascrobat increased plant height, photosynthesis, number spikelet per spike, 1000-grain weight (TKW) and grain yield in comparison with other treatments so that the priming with Ascrobat increased grain yield by 9% compared with control. Finally with regard to the compare mean of interaction between treatments it can be concluded, seed priming with Ascrobat modified the effect of drought stress.