Role of Vagal Innervation in Diurnal Rhythm of Intestinal Peptide Transporter 1 (PEPT1)

Qandeel, Hisham G.; Alonso, Fernando; Hernandez, David J.; Duenes, Judith A.; Zheng, Ye; Scow, Jeffrey S.; Sarr, Michael G.

doi:10.1007/s11605-009-0984-6

Cited by 23 publications

(25 citation statements)

References 37 publications

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“…Daily weights of the rat and the chow consumed (per light/dark cycle) were tabulated until the time of harvest. Because gene expression and transport function of PepT1 are known to vary diurnally [18][19][20][21], a set time-point of the light/dark cycle (3PM) was chosen to harvest the intestinal tissue; we selected this time-point, because we have shown previously that gene expression and transport function of PepT1 peak 3 h before the dark cycle [20][21].…”

Section: Methodsmentioning

confidence: 99%

“…All tissue was harvested at a fixed time of the day due to known diurnal patterns in expression and function of PepT1 [20][21]. At the time of tissue harvest, rats were anesthetized with inhaled isoflurane and intra-peritoneal pentobarbital.…”

Section: Tissue Harvestmentioning

confidence: 99%

“…Real-time polymerase chain reaction (RT-PCR) was used to quantitate mRNA levels of PepT1 as described previously [20][21]. Mucosal samples stored in RNA stabilization buffer were thawed on ice, and homogenized RNA was isolated using the RNeasy Midi kit (Qiagen).…”

Section: Mrna Measurementmentioning

confidence: 99%

“…Levels of total cellular protein for PepT1 were measured semi-quantitatively using our wellcharacterized technique with Western blots [14,[20][21]. Tissue samples stored in RIPA buffer containing Halt protease inhibitors and PMSF were thawed on ice; the presence of protease inhibitors was used to minimize protein degradation.…”

Section: Protein Measurementmentioning

confidence: 99%

“…Absorption of short peptides and peptide-like substances under normal conditions occurs predominantly in the proximal intestine (primarily the jejunum), with the ileum absorbing a substantially lesser amount of peptides. Colonic absorption of peptides in the normal physiologic state is insignificant [17][18][19][20][21]. Our aim in this study was to identify the mechanisms of ileal adaptation (and colonic adaptation, if any) for peptide absorption after massive, proximal small bowel resection.…”

Section: Introductionmentioning

confidence: 99%

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Peptide absorption after massive proximal small bowel resection: Mechanisms of ileal adaptation

Qandeel

Hernandez

Alonso

et al. 2009

Journal of the American College of Surgeons

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Section: Methodsmentioning

confidence: 99%

Section: Tissue Harvestmentioning

confidence: 99%

Section: Mrna Measurementmentioning

confidence: 99%

Section: Protein Measurementmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Peptide absorption after massive proximal small bowel resection: Mechanisms of ileal adaptation

Qandeel

Hernandez

Alonso

et al. 2009

Journal of the American College of Surgeons

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Di- and tripeptide transport in vertebrates: the contribution of teleost fish models

et al. 2016

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Solute Carrier 15 (SLC15) family, alias H-coupled oligopeptide cotransporter family, is a group of membrane transporters known for their role in the cellular uptake of di- and tripeptides (di/tripeptides) and peptide-like molecules. Of its members, SLC15A1 (PEPT1) chiefly mediates intestinal absorption of luminal di/tripeptides from dietary protein digestion, while SLC15A2 (PEPT2) mainly allows renal tubular reabsorption of di/tripeptides from ultrafiltration, SLC15A3 (PHT2) and SLC15A4 (PHT1) possibly interact with di/tripeptides and histidine in certain immune cells, and SLC15A5 has unknown function. Our understanding of this family in vertebrates has steadily increased, also due to the surge of genomic-to-functional information from 'non-conventional' animal models, livestock, poultry, and aquaculture fish species. Here, we review the literature on the SLC15 transporters in teleost fish with emphasis on SLC15A1 (PEPT1), one of the solute carriers better studied amongst teleost fish because of its relevance in animal nutrition. We report on the operativity of the transporter, the molecular diversity, and multiplicity of structural-functional solutions of the teleost fish orthologs with respect to higher vertebrates, its relevance at the intersection of the alimentary and osmoregulative functions of the gut, its response under various physiological states and dietary solicitations, and its possible involvement in examples of total body plasticity, such as growth and compensatory growth. By a comparative approach, we also review the few studies in teleost fish on SLC15A2 (PEPT2), SLC15A4 (PHT1), and SLC15A3 (PHT2). By representing the contribution of teleost fish to the knowledge of the physiology of di/tripeptide transport and transporters, we aim to fill the gap between higher and lower vertebrates.

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Intestinal Adaptation for Oligopeptide Absorption via PepT1 After Massive (70%) Mid-Small Bowel Resection

Madhavan

Scow

Chaudhry

et al. 2011

Journal of Gastrointestinal Surgery

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Introduction Proteins are absorbed primarily as short peptides via PepT1. Hypothesis Intestinal adaptation for peptide absorption after massive mid-small intestinal resection occurs by increased expression of PepT1 in the remnant small intestine and colon. Methods Peptide uptake was measured in duodenum, jejunum, ileum, and colon using Glycyl-Sarcosine 1 wk (n=9) and 4 wk (n=11) after 70% mid-small bowel resection, and in corresponding segments from unoperated rats (n=12) and after transection and reanastomosis of jejunum and ileum (n=8). Expression of PepT1 (mRNA, protein) and villus height were measured. Results Intestinal transection/reanastomosis did not alter gene expression. Compared to non-operated controls, 70% mid-small bowel resection increased jejunal peptide uptake (p<0.05) associated with increased villus height (1.13 vs 1.77 and 1.50 mm resp, p<0.01). In ileum although villus height increased at 1 and 4 wk (1.03 vs 1.21 and 1.35 mm resp; p<0.01), peptide uptake was not altered. PepT1 mRNA and protein were decreased at 1 wk, and PepT1 protein continued low at 4 wk. Gene expression, peptide uptake, and histomorphology were unchanged in the colon. Conclusions Jejunal adaptation for peptide absorption occurs by hyperplasia. Distal Ileum and colon do not have a substantive role in adaptation for peptide absorption.

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Role of Vagal Innervation in Diurnal Rhythm of Intestinal Peptide Transporter 1 (PEPT1)

Abstract: BACKGROUND: Protein is absorbed predominantly as di/tripeptides via H + /peptide cotransporter-1 (PEPT1). We demonstrated previously diurnal variations in expression and function of duodenal and jejunal but not ileal PEPT1; neural regulation of this pattern is unexplored.

Cited by 23 publications

References 37 publications

Peptide absorption after massive proximal small bowel resection: Mechanisms of ileal adaptation

Peptide absorption after massive proximal small bowel resection: Mechanisms of ileal adaptation

Di- and tripeptide transport in vertebrates: the contribution of teleost fish models

Intestinal Adaptation for Oligopeptide Absorption via PepT1 After Massive (70%) Mid-Small Bowel Resection

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