Role of vascular endothelial growth factor expression in pathogenesis of postmenopausal osteoporosis

Nasser, Mahmoud El Tayeb; Khaled, Henaz Farouk; Kaddah, Eman A; Elbadrawy, Ahmed M; Mahdi, Sahar M; Sharobeem, Mary A

doi:10.4103/1110-161x.123809

Cited by 4 publications

(3 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The mean area of trabecular bone, the mean thickness of the outer cortical bone, and the mean number of osteocytes were determined ( Zhou et al, 2015 ). Additionally, the mean percentage of collagen fiber density ( Nasser et al, 2013 ) and the percentage of cells expressing VEGF and PCNA when analyzed at magnification were detected.…”

Section: Methodsmentioning

confidence: 99%

Estradiol and zinc-doped nano hydroxyapatite as therapeutic agents in the prevention of osteoporosis; oxidative stress status, inflammation, bone turnover, bone mineral density, and histological alterations in ovariectomized rats

et al. 2022

View full text Add to dashboard Cite

Osteoporosis (OP) is a serious health problem, and the most popular therapeutic strategy for OP is hormone replacement (estrogen); however, it increases the risk of reproductive cancers. Hydroxyapatite (HA) nanoparticles have a similar chemical structure to the bone mineral component and can be used as a new remedy for OP. This study was designed to investigate the osteoporosis-protective potential of nano zinc hydroxyapatite (ZnHA-NPs) and/or estradiol (E2) combined therapy. A total of 35 adult female rats were assigned into five groups (n = 7): 1) control group; 2) ovariectomized group (OVX); 3) OVX received oral estradiol replacement therapy (OVX/E2); 4) OVX received ZnHA replacement therapy (OVX/ZnHA); and 5) OVX received both estradiol and ZnHA-NPs combined therapy (OVX/E2+ZnHA). After 3 months of treatment, serum bone markers and estrogen level, oxidative/antioxidant, and inflammatory cytokines were determined. Additionally, femoral expression of estrogen receptors alpha and beta (ESR1; ESR2), receptor activator of nuclear factor-kappa B (RANKL) ligand, osteoprotegerin (OPG), bone mineral density (BMD), histological alterations, and immunohistochemical expression of vascular endothelial growth factor (VEGF) and proliferating cell nuclear antigen (PCNA) were assessed. ALP, PINP, Ca, and P concentrations improved significantly (p < 0.05) in all treatment groups, especially in the OVX/E + ZnHA group. MDA and NO were higher in OVX rats, while SOD activity and GSH were lower (p < 0.05). E2 alone or with ZnHA-NPs restored the estimated antioxidant molecules and cytokines toward normal levels in OVX rats (p < 0.05). On the other hand, E2 and ZnHA increased OPG and OC expression in femurs while decreasing ESR1, ESR2, and NF-kB expression (p < 0.05). The combination treatment was superior in the restoration of normal femoral histoarchitecture and both cortical and trabecular BMD (p < 0.05). Overall, the combined therapy of OVX/E2+ZnHA was more effective than the individual treatments in attenuating excessive bone turnover and preventing osteoporosis.

show abstract

Section: Methodsmentioning

confidence: 99%

Estradiol and zinc-doped nano hydroxyapatite as therapeutic agents in the prevention of osteoporosis; oxidative stress status, inflammation, bone turnover, bone mineral density, and histological alterations in ovariectomized rats

et al. 2022

View full text Add to dashboard Cite

show abstract

“…After fixation with a 20% neutrally buffered formalin solution (Wako Pure Chemical Industries, Osaka, Japan), each tissue specimen was embedded using Osteoresin (an embedding kit; Wako Pure Chemical Industries, Osaka, Japan), sectioned using a saw microtome (SP1600, Leica AG, Glattbrugg, Switzerland), stained with hematoxylin-eosin, and then examined under a light microscope. Trabeculae containing osteocytes (intensely stained by eosin, with nuclei intensely stained by hematoxylin) and hematopoietic marrow within trabecullar bone (nucleus stained purple by hematoxylin, cytoplasm stained pink by eosin) [ 28 ][ 29 ] were examined. All experimental protocols were approved by the institutional animal care committee of Shinshu University.…”

Section: Methodsmentioning

confidence: 99%

A three-dimensional block structure consisting exclusively of carbon nanotubes serving as bone regeneration scaffold and as bone defect filler

et al. 2017

View full text Add to dashboard Cite

Many recent studies have been conducted to assess the ability of composite materials containing carbon nanotubes (CNTs) with high bone affinity to serve as scaffolds in bone regenerative medicine. These studies have demonstrated that CNTs can effectively induce bone formation. However, no studies have investigated the usefulness of scaffolds consisting exclusively of CNTs in bone regenerative medicine. We built a three-dimensional block entity with maximized mechanical strength from multi-walled CNTs (MWCNT blocks) and evaluated their efficacy as scaffold material for bone repair. When MWCNT blocks containing recombinant human bone morphogenetic protein-2 (rhBMP-2) were implanted in mouse muscle, ectopic bone was formed in direct contact with the blocks. Their bone marrow densities were comparable to those of PET-reinforced collagen sheets with rhBMP-2. On day 1 and day 3, MC3T3-E1 preosteoblasts were attached to the scaffold surface of MWCNT blocks than that of PET-reinforced collagen sheets. They also showed a maximum compression strength comparable to that of cortical bone. Our MWCNT blocks are expected to serve as bone defect filler and scaffold material for bone regeneration.

show abstract

“…The cellular activities of osteoblasts and osteoclasts have been proven to be positively impacted by the interaction with zinc-related proteins ( Figure 2 ) and, therefore, to result in the decrease of early stage bone resorption and deterioration [ 115 ]. Alkaline phosphatase utilizes zinc as a co-factor as the process of bone mineralization gradually increases [ 116 , 117 , 118 ]. Zn complements the bioactivity of HAP [ 119 ], as zinc-enforced tricalcium phosphate is administered in order to promote osteogenesis in osteoporotic bone [ 120 , 121 ], in comparison with simple HAPs [ 122 ].…”

Section: Hydroxyapatite Nanoformulationsmentioning

confidence: 99%

Recent Trends in Hydroxyapatite Supplementation for Osteoregenerative Purposes

et al. 2023

View full text Add to dashboard Cite

Bone regeneration has gained attention in the biomedical field, which has led to the development of materials and synthesis methods meant to improve osseointegration and cellular bone activity. The properties of hydroxyapatite, a type of calcium phosphate, have been researched to determine its advantages for bone tissue engineering, particularly its biocompatibility and ability to interact with bone cells. Recently, the advantages of utilizing nanomolecules of hydroxyapatite, combined with various substances, in order to enhance and combine their characteristics, have been reported in the literature. This review will outline the cellular and molecular roles of hydroxypatite, its interactions with bone cells, and its nano-combinations with various ions and natural products and their effects on bone growth, development, and bone repair.

show abstract

Role of vascular endothelial growth factor expression in pathogenesis of postmenopausal osteoporosis

Cited by 4 publications

References 29 publications

Estradiol and zinc-doped nano hydroxyapatite as therapeutic agents in the prevention of osteoporosis; oxidative stress status, inflammation, bone turnover, bone mineral density, and histological alterations in ovariectomized rats

Estradiol and zinc-doped nano hydroxyapatite as therapeutic agents in the prevention of osteoporosis; oxidative stress status, inflammation, bone turnover, bone mineral density, and histological alterations in ovariectomized rats

A three-dimensional block structure consisting exclusively of carbon nanotubes serving as bone regeneration scaffold and as bone defect filler

Recent Trends in Hydroxyapatite Supplementation for Osteoregenerative Purposes

Contact Info

Product

Resources

About