Role of VEGF Polymorphisms in the Susceptibility and Severity of Interstitial Lung Disease

Remuzgo‐Martínez, Sara; Genre, Fernanda; Pulito‐Cueto, Verónica; Atienza‐Mateo, Belén; Cuesta, Víctor Manuel Mora; Iturbe-Fernández, David; Rozas, Sonia María Fernández; Lera‐Gómez, Leticia; Lecue, Pilar Alonso; Ussetti, M.; Laporta, Rosalía; Berastegui, Cristina; Solé, Amparó; Pérez, Virginia; Gafas, Alicia de Pablo; Gualillo, Oreste; Cifrián, José Manuel; López‐Mejías, Raquel

doi:10.3390/biomedicines9050458

Cited by 5 publications

(4 citation statements)

References 34 publications

(54 reference statements)

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“…The study of biomarkers as additional tools in the clinical practice for the diagnosis and severity of ILD has aroused great interest in the last years [ 17 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 ]. In this regard, the role of ET-1 in the pathogenesis of a large and well-defined cohort of ILD patients remains to be investigated in depth.…”

Section: Discussionmentioning

confidence: 99%

Endothelin-1 as a Biomarker of Idiopathic Pulmonary Fibrosis and Interstitial Lung Disease Associated with Autoimmune Diseases

Pulito‐Cueto

Genre

López‐Mejías

et al. 2023

IJMS

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The aim of this study was to determine the role of endothelin-1 (ET-1), a molecule involved in multiple vascular and fibrosing abnormalities, as a biomarker of interstitial lung disease (ILD), as well as its use for the differential diagnosis between idiopathic pulmonary fibrosis (IPF) and ILD associated with autoimmune diseases (AD-ILD), using a large and well-defined cohort of patients with ILD. A total of 112 patients with IPF, 91 patients with AD-ILD (28 rheumatoid arthritis (RA), 26 systemic sclerosis, 20 idiopathic inflammatory myositis and 17 interstitial pneumonia with autoimmune features) and 44 healthy controls were included. ET-1 serum levels were determined by enzyme-linked immunosorbent assay. A significant increase in ET-1 levels was found in patients with IPF compared to controls. Likewise, AD-ILD patients also showed higher ET-1 levels than controls when the whole cohort was stratified by the type of AD. Similar ET-1 levels were found in IPF and AD-ILD patients, regardless of the underlying AD. Interestingly, increased ET-1 levels were correlated with worse lung function in IPF and RA-ILD patients. Our study supports that serum ET-1 may be useful as a biomarker of ILD, although it could not help in the differential diagnosis between IPF and AD-ILD. Moreover, ET-1 levels may be associated with ILD severity.

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Section: Discussionmentioning

confidence: 99%

Endothelin-1 as a Biomarker of Idiopathic Pulmonary Fibrosis and Interstitial Lung Disease Associated with Autoimmune Diseases

Pulito‐Cueto

Genre

López‐Mejías

et al. 2023

IJMS

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“…VEGF levels were measured in serum samples by a commercial quantitative colorimetric sandwich enzyme-linked immunosorbent assay (Reddot Biotech Inc., Kelowna, BC, Canada) as previously described [35].…”

Section: Vegf Serum Levels Determinationmentioning

confidence: 99%

Angiogenic T Cells: Potential Biomarkers for the Early Diagnosis of Interstitial Lung Disease in Autoimmune Diseases?

et al. 2022

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(1) Background: We explored, for the first time, the contribution of angiogenic T cells (TAng) in interstitial lung disease associated to autoimmune disease (AD-ILD+) as potential biomarkers of the disease, evaluating their role in the underlying vasculopathy and lung fibrosis. Additionally, the relationship of TAng with clinical manifestations and cellular and molecular endothelial dysfunction-related biomarkers was assessed. (2) Methods: We included 57 AD-ILD+ patients (21 with rheumatoid arthritis (RA)-ILD+, 21 with systemic sclerosis (SSc)-ILD+ and 15 with other AD-ILD+) and three comparative groups: 45 AD-ILD- patients (25 RA-ILD- and 20 SSc-ILD−); 21 idiopathic pulmonary fibrosis (IPF) patients; 21 healthy controls (HC). TAng were considered as CD3+CD184+CD31+ by flow cytometry. (3) Results: A similar TAng frequency was found between AD-ILD+ and IPF, being in both cases lower than that observed in AD-ILD- and HC. A lower TAng frequency was associated with negative Scl-70 status and lower FEV1/FVC ratio in SSc-ILD+, as well as with men in RA-ILD+ and non-specific interstitial pneumonia radiological pattern in other AD-ILD+. No relationship between TAng and endothelial progenitor cells, endothelial cells and vascular endothelial growth factor gene expression and protein levels was disclosed. (4) Conclusions: Our findings suggest TAng as potential biomarkers for the early diagnosis of ILD in AD.

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“…Genomic DNA was extracted from peripheral blood using the REALPURE "SSS" kit (RBME04, REAL, Durviz S.L., Valencia, Spain), as previously described (28). All patients were genotyped for IFNG rs2069718 G/A and rs1861493 A/G, as well as for IFNGR1 rs1327474 G/A by TaqMan assays, previously assessed in several autoimmune conditions (17)(18)(19)(20).…”

Section: Ifng and Ifngr1 Polymorphisms Selection And Genotypingmentioning

confidence: 99%

Cranial and extracranial large-vessel giant cell arteritis share a genetic pattern of interferon-gamma pathway

Prieto-Peña

Genre

Pulito‐Cueto

et al. 2022

Clinical and Experimental Rheumatology

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ObjectiveTwo main different clinical phenotypes of giant cell arteritis (GCA) have been described, the classic cranial pattern and the extracranial large-vessel (LV) pattern. Since interferon gamma (IFNG) has shown to be a pivotal cytokine in the pathophysiology of GCA, our aim was to evaluate for the first time the influence of IFNG and IFNG receptor 1 (IFNGR1) polymorphisms in the different clinical phenotypes of GCA. Methods Two IFNG polymorphisms (rs2069718 G/A and rs1861493 A/G) and one polymorphism in IFNGR1 (rs1327474 G/A) weregenotyped in 191 patients with biopsy-proven cranial GCA, 109 with extracranial LV-GCA and 490 healthy controls. A comparative study was conducted between patients with cranial and extracranial LV-GCA. ResultsNo significant differences in genotype, allele, and haplotype frequencies of IFNG polymorphisms were found between GCA patients with the classic cranial pattern and the extracranial LV-GCA pattern. Similar results were found for genotype and allele frequencies of IFNGR1 polymorphism. It was also the case when patients with extracranial LV-GCA were compared with healthy controls. ConclusionOur results show that IFNG and IFNGR1 polymorphisms do not influence the clinical phenotype of expression of GCA.Classic cranial GCA and extracranial LV-GCA seem to share a genetic pattern of IFNG pathway.

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Role of VEGF Polymorphisms in the Susceptibility and Severity of Interstitial Lung Disease

Cited by 5 publications

References 34 publications

Endothelin-1 as a Biomarker of Idiopathic Pulmonary Fibrosis and Interstitial Lung Disease Associated with Autoimmune Diseases

Endothelin-1 as a Biomarker of Idiopathic Pulmonary Fibrosis and Interstitial Lung Disease Associated with Autoimmune Diseases

Angiogenic T Cells: Potential Biomarkers for the Early Diagnosis of Interstitial Lung Disease in Autoimmune Diseases?

Cranial and extracranial large-vessel giant cell arteritis share a genetic pattern of interferon-gamma pathway

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