Role of VIP and Substance P in NANC Innervation in the Longitudinal Smooth Muscle of the Rat Jejunum—Influence of Extrinsic Denervation

Kasparek, Michael S.; Fatima, Javairiah; Iqbal, Corey W.; Duenes, Judith A.; Sarr, Michael G.

doi:10.1016/j.jss.2007.01.021

Cited by 26 publications

(77 citation statements)

References 35 publications

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“…The role of VIP (and NO) in the control of enteric contractile activity is complex, incompletely understood, and varies sometimes markedly between species and across anatomic regions. 17 In jejunal longitudinal muscle of control rats, exogenous VIP has no apparent effect on spontaneous contractile activity; 16,21,22 in contrast, others have shown that VIP has an inhibitory effect on circular muscle of rat jejunum, 19,20 which is consistent with our current study showing a profound, dose-dependent inhibition of contractile activity by exogenous VIP 1 year after SBT. This result, however, is in conflict with a study from Tomita et al, 19 which suggested that the sensitivity to exogenous VIP was decreased 18 week after SBT in circular muscle of rat jejunum.…”

Section: Discussionsupporting

confidence: 91%

“…Both neuropeptides play an important role in enteric motor innervation, such as the peristaltic reflex, 15 and might therefore contribute to the enteric motor dysfunction after SBT. Previous studies from our laboratory showed a decrease in sensitivity to exogenous substance P and a diminished endogenous release of VIP 8 weeks after SBT in the longitudinal muscle of rat jejunum; 16 in addition, a decreased sensitivity to substance P persisted 1 year after SBT despite extrinsic reinnervation. 17 Coordinated enteric motor function is the result of the interplay of the longitudinal and circular muscle layers.…”

Section: Introductionmentioning

confidence: 52%

“…7,11 In a previous study in longitudinal muscle of rat jejunum, EFS at low frequencies inhibited typically contractile activity during the first 4-6 s of EFS after which some contractile activity recurred. 16 Because of this pattern, we evaluated the effect of EFS separately not only for the entire 10 s of EFS but also for the first 4 s and the last 6 s of EFS. Contractile activity measured as area under the contractile curve was expressed as the percent of baseline contractile activity for an equally long interval measured during the 40 s immediately before EFS.…”

Section: Discussionmentioning

confidence: 99%

“…Staining for TH-positive neurons was present in duodenum and jejunum of NC and SBT with comparable immunopositivity, although we have shown previously that TH staining is absent in the jejunoileum early (≤2 months) after SBT. 16 No subjective differences were evident in the jejunum between NC and SBT.…”

Section: Immunohistofluorescence Microscopymentioning

confidence: 93%

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Long-Term Effects of Extrinsic Denervation on VIP and Substance P Innervation in Circular Muscle of Rat Jejunum

Kasparek

Fatima

Iqbal

et al. 2007

Journal of Gastrointestinal Surgery

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Intestinal denervation contributes to enteric motor dysfunction after small bowel transplantation (SBT). Our aim was to determine long-term effects of extrinsic denervation on function of nonadrenergic, noncholinergic innervation with substance P and vasoactive intestinal polypeptide (VIP). Contractile activity of jejunal circular muscle strips from six age-matched, naive control rats (NC) and eight rats 1 year after syngeneic SBT was studied in tissue chambers. Spontaneous contractile activity did not differ between groups. Exogenous VIP inhibited contractile activity dose-dependently to a comparable degree in both groups. The VIP antagonist ([D-p-Cl-Phe(6),Leu(17)]-VIP) and the nitric oxide synthase inhibitor L-NG-nitro-arginine did not affect VIP-induced inhibition but increased contractile activity during electrical field stimulation (EFS) in both groups. Exogenous substance P increased contractile activity dose-dependently, greater in NC than SBT. The substance P antagonist ([D-Pro(2),D-Trp(7,9)]-substance P) inhibited effects of exogenous substance P and decreased the excitatory EFS response. Immunohistofluorescence showed tyrosine hydroxylase staining after SBT indicating sympathetic reinnervation. In jejunal circular muscle after chronic denervation, response to exogenous substance P, but not VIP, is decreased, whereas endogenous release of both neurotransmitters is preserved. Alterations in balance of excitatory and inhibitory pathways occur despite extrinsic reinnervation and might contribute to enteric motor dysfunction after SBT.

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Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 52%

Section: Discussionmentioning

confidence: 99%

Section: Immunohistofluorescence Microscopymentioning

confidence: 93%

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Long-Term Effects of Extrinsic Denervation on VIP and Substance P Innervation in Circular Muscle of Rat Jejunum

Kasparek

Fatima

Iqbal

et al. 2007

Journal of Gastrointestinal Surgery

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“…VIP is above all known as an inhibitory neurotransmitter and/or neuromodulator, which causes the muscle hyperpolarsation and relaxation [19]. Moreover, VIP takes part in the blood flow regulation [18] and alters secretory actions of the GI tract.…”

Section: Discussionmentioning

confidence: 99%

Co-localisation of cocaine- and amphetamine-regulated transcript peptide and vasoactive intestinal polypeptide in the myenteric plexus of the porcine transverse colon

Palus

Rytel

2013

Folia Morphol

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Cocaine-and amphetamine-regulated transcript peptide (CART) is a substance, which can play the role of neuromediator and/or neuromodulator in nerve structures within the gastrointestinal tract. However knowledge concerning its functions and co-localisation with other neuronal factors is rather scarce. During the present investigation the co-localisation of CART and vasoactive intestinal polypeptide (VIP) in the neurons of meyenteric plexus within the porcine transverse colon was studied using double immunofluorescence technique and semiquantitative arbitrary scale of the frequency of presence CART+/VIP+, CART+/VIP-and CART-/VIP+ neuronal cells. The most often (+++) CART-/VIP+ neurons were encountered, neurons simultaneously immunoreactive to CART and (Folia Morphol 2013; 72, 4: 328-332)

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Mechanisms of Action of the Gasotransmitter Hydrogen Sulfide in Modulating Contractile Activity of Longitudinal Muscle of Rat Ileum

Nagao¹,

Linden

Duenes³

et al. 2011

Journal of Gastrointestinal Surgery

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AIM To determine mechanisms of action of the gasotransmitter hydrogen sulfide (H2S) on contractile activity in longitudinal muscle of rat ileum. METHODS Ileal longitudinal muscle strips were prepared to measure isometric contractions. Effects of sodium hydrosulfide (NaHS), a donor of H2S, were evaluated on spontaneous contractile activity and after enhanced contractile activity with bethanechol. L-cysteine was evaluated as a potential endogenous donor of H2S. We evaluated involvement of extrinsic nerves, enteric nervous system, visceral afferent nerves, nitric oxide, and K+ATP channel and K+Ca channel activity on the action of H2S using non-adrenergic/non-cholinergic conditions, tetrodotoxin, capsaicin, L-NG-nitro arginine (L-NNA), glibenclamide, and apamin, respectively, as well as electrical field stimulation. RESULT NaHS dose-dependently and reversibly inhibited spontaneous and bethanechol-stimulated contractile activity (p<0.05). L-cysteine had no inhibitory effect. Non-adrenergic/non-cholinergic conditions, tetrodotoxin, capsaicin, L-NNA, glibenclamide, or apamin had no major effect on total contractile activity by NaHS, although both tetrodotoxin and apamin decreased the frequency of bethanechol-enhanced contractile activity (p<0.05). We could not demonstrate H2S release by electrical field stimulation but did show that inhibition of cystathionine β synthase, an endogenous source of H2S, augmented the inhibitory effect of low-frequency electrical field stimulation. CONCLUSION H2S inhibits contractile activity of ileal longitudinal muscle dose-dependently but not through pathways mediated by the extrinsic or enteric nervous system, visceral afferent nerves, nitric oxide, K+ATP channels, or K+Ca channels.

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Role of VIP and Substance P in NANC Innervation in the Longitudinal Smooth Muscle of the Rat Jejunum—Influence of Extrinsic Denervation

Cited by 26 publications

References 35 publications

Long-Term Effects of Extrinsic Denervation on VIP and Substance P Innervation in Circular Muscle of Rat Jejunum

Long-Term Effects of Extrinsic Denervation on VIP and Substance P Innervation in Circular Muscle of Rat Jejunum

Co-localisation of cocaine- and amphetamine-regulated transcript peptide and vasoactive intestinal polypeptide in the myenteric plexus of the porcine transverse colon

Mechanisms of Action of the Gasotransmitter Hydrogen Sulfide in Modulating Contractile Activity of Longitudinal Muscle of Rat Ileum

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