Role of Vitamin E in Mitigating Methomyl Induced Acute Toxicity in Blood of Male Wistar Rats

Garg, Deepak; Kiran, Ravi; Bansal, Anil Kumar; Malhotra, Anshoo; Dhawan, D. K.

doi:10.1080/01480540802390775

Cited by 12 publications

(6 citation statements)

References 26 publications

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“…However, the significant decrease in the hepatic and renal MDA content confirms that the pretreatment with vitamin E and taurine could effectively protect against the hepatic and renal lipid peroxidation induced by MC. These findings are in accordance with the study of Garg et al (2008), who reported that the pretreatment with vitamin E decreased the levels of MDA after single dose of methomyl administration in rat erythrocytes.…”

Section: Discussionsupporting

confidence: 93%

“…Pretreatment of vitamin E showed decrease in the activities of the SOD and CAT, which indicates that vitamin E and taurine provide protection against MC-induced oxidative stress. Similar results were also observed by Garg et al (2008), who reported that the pretreatment with vitamin E moderated the activity of SOD after single dose of methomyl administration in rat erythrocytes.…”

Section: Discussionsupporting

confidence: 89%

“…Vitamin E is the most effective chain breaking lipid soluble antioxidant in the biological membranes and protects cellular structures against the damage of oxygen free radicals and reactive products of lipid peroxidation (Sies and Murphy, 1991). To date, several studies indicated that vitamin E inhibits lipid peroxidation and attenuated the antioxidant enzyme activities following pesticide exposures (Aldana et al, 2001; Atessahin et al, 2005; Durak et al, 2009; el-Demerdash et al, 2004; Garg et al, 2008; Giray et al, 2001; John et al, 2001; Kalender et al, 2004; 2005; Sodhi et al, 2008; Uzun et al, 2009; Yousef et al, 2006). Taurine (2-aminoethanesulfonic acid), a ubiquitous free amino acid, is present in most mammalian tissues.…”

Section: Introductionmentioning

confidence: 99%

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Acute effects of methiocarb on oxidative damage and the protective effects of vitamin E and taurine in the liver and kidney of Wistar rats

et al. 2012

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Methiocarb (MC) is a widely used carbamate pesticide in agriculture and health programs. Although the main molecular mechanism of carbamate toxicity involves acetylcholinesterase inhibition, studies have also implicated the induction of oxidative stress. Therefore, the present study was aimed to evaluate the effect of acute MC exposure on lipid peroxidation, antioxidant defense systems, histological changes in Wistar rats and the protective effect of pretreatment with vitamin E and taurine. A total of 48 rats were randomly divided into six groups. Rats in group I were given corn oil, while those in group III were dosed with vitamin E (100 mg/kg body weight (b.w.)) and in group V were dosed with taurine (50 mg/kg b.w.). Rats in group II were administered with MC only (25 mg/kg b.w., 1/4 of median lethal dose (LD(50))), while those in groups IV and VI were pretreated with vitamin E (100 mg/kg b.w.) and taurine (50 mg/kg b.w.) for 20 days, respectively, and then exposed to MC (25 mg/kg b.w.). The rats administered with MC showed significant increase in the levels of malondialdehyde in the liver and kidney as an index of lipid peroxidation. Levels of glutathione and activities of superoxide dismutase, catalase and glutathione peroxidase were significantly increased, while activity of glutathione reductase remained unchanged in both the tissues after MC treatment. Mild degenerative histological changes were observed in liver tissue, while the changes in kidney tissue were more severe then liver after MC treatment. Pretreatment with vitamin E and taurine resulted in a significant decrease in the lipid peroxidation and alleviating effects on antioxidant defense systems in both the tissues, while protective effects on the histological changes were shown only in kidney when compared with liver. In conclusion, the study has demonstrated that the acute MC exposure in Wistar rats caused oxidative damage on liver and kidney, which were partly ameliorated by the pretreatment of vitamin E and taurine.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 89%

Section: Introductionmentioning

confidence: 99%

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Acute effects of methiocarb on oxidative damage and the protective effects of vitamin E and taurine in the liver and kidney of Wistar rats

et al. 2012

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“…In agreement with these results, Mansour et al found that methomyl decreased the activity of SOD, GST and increased LPO in rat erythrocytes [34]. According to Garg et al acute 24 hr single oral dose of 9 mg/kg of methomyl treatment to rats resulted in a significant increase in the LPO, further GSH levels and the activities of Catalase and GST were found to be significantly decreased following methomyl treatment [68]. Similarly El-Khawaga found that methomyl decreased the activity of SOD, CAT and GST in mice liver [24], and Salama et al (2005) reported that methomyl mode of action could be due to the induction of oxidative stress [69].…”

Section: Histologic Studiesmentioning

confidence: 61%

Methomyl induced alteration in mice hepatic-oxidative status

Bb¹,

Manawadi²

2010

Int J of Biot Appl

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Methomyl (S-methyl-1-N (methyl carbamoyl) oxy] thio acetimidate, is a N -methyl carbamate broad spectrum insecticide. The methomyl containing technical formulation the "Lannate" was evaluated for its effects on the liver antioxidant contents, oxidative stress by-products and oxidative stress enzyme activities and histopathology in Swiss albino mice. Normal virgin male Swiss albino mice of 90 days old weighing about 25-30g were used in the experiment. The mice were administered 1, 2, 3, and 4mg/kg body wt methomyl for 30 days and effective dose 4 mg for 5, 10, and 20 days to know the dose and durational effect on liver. The mice were sacrificed on day 31 st or 24 hours after the terminal exposure. Liver dissected out freed from adherent tissue and weighed to nearest milligram. The liver histology, estimations of antioxidant contents, oxidative stress by-products and oxidative stress enzyme activities were carried out. Liver antioxidant contents showed that levels of GSH (Glutathione) and ascorbic acid were decreased significantly in mice treated with 2, 3 and 4mg/kg/day methomyl and 4 mg/ kg/ day for 10 and 20days of methomyl treatment, except the level of ascorbic acid was not changed significantly in mice treated with 2 mg/kg body wt. Liver antioxidant products showed that levels of TBARS (thiobarbaturic acid) and protein carbonyl were increased significantly in mice treated with 2, 3 and 4mg/kg/day methomyl and 4 mg/ kg/ day for 10 and 20days of methomyl treatment, except the level of TBARS was not changed significantly in mice treated with 2 mg/kg body wt of methomyl. Liver oxidative stress enzyme activities showed that levels of CAT (Catalase), SOD (super oxide dismutase) and GST (Glutathione-s-transferase) were decreased significantly in mice treated with 2, 3 and 4mg/kg/day methomyl and 4 mg/ kg/ day for 10 and 20days of methomyl treatment. The Histology of liver of mice treated with 2, 3 and 4 mg / kg/ day methomyl and 4 mg / kg/ day for 10 and 20days of duration showed that dilation of central vein, sinusoids between hypertrophied hepatocytes and cytoplasmic vacuolization with loss of radial arrangement of cells. The result of the present study suggests that chronic exposure to methomyl insecticide has deleterious effect on liver. The study also revealed that the methomyl might have affected cell metabolism and cell membrane permeability and detoxification system in liver.

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“…The anemia observed might have been due to the ability of the methomyl to cause acute hemolysis or it might be due to cause oxidative stress, these changes due to an increase rate of breakdown of red cells and/or the toxic effect of methomyl on bone marrow. (14). The reduction in Hb content may be due to increased rate of breakdown of red cells and/or reduction in the rate of formation of RBC's.…”

Section: Discussionmentioning

confidence: 99%

Study of Effect of Methomyl on Some Hematological, Biochemical Parameters and Histological Changes in Male Albino Rats

Maulood¹,

Shekha²

2017

DJPS

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Role of Vitamin E in Mitigating Methomyl Induced Acute Toxicity in Blood of Male Wistar Rats

Cited by 12 publications

References 26 publications

Acute effects of methiocarb on oxidative damage and the protective effects of vitamin E and taurine in the liver and kidney of Wistar rats

Acute effects of methiocarb on oxidative damage and the protective effects of vitamin E and taurine in the liver and kidney of Wistar rats

Methomyl induced alteration in mice hepatic-oxidative status

Study of Effect of Methomyl on Some Hematological, Biochemical Parameters and Histological Changes in Male Albino Rats

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