ROLE OF VlsE/C6 ANTIGEN AS A MARKER FOR EARLY LYME BORRELIOSIS
DIAGNOSIS AND MONITORING THE EFFECTIVENESS OF ITS TREATMENT

Krzemień, Paweł

doi:10.5114/hpc.2017.69023

Cited by 5 publications

(7 citation statements)

References 19 publications

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“…Great importance for the diagnosis of Lyme disease is attached to the quality of Borreliella antigens. The use of recombinant antigens (p100, p58, p41, VlsE, OspC, DbpA), especially in the Western blot test, instead of antigens from cell lysates, is diagnostically preferable [7,25,28].…”

Section: Discussionmentioning

confidence: 99%

Analysis of selected serological parameters in patients with diagnosed Lyme borreliosis and in seropositive patients with no clinical symptoms

Tokarska-Rodak¹,

Pańczuk²,

Fota-Markowska³

et al. 2021

Ann Agric Environ Med.

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Section: Discussionmentioning

confidence: 99%

Analysis of selected serological parameters in patients with diagnosed Lyme borreliosis and in seropositive patients with no clinical symptoms

Tokarska-Rodak¹,

Pańczuk²,

Fota-Markowska³

et al. 2021

Ann Agric Environ Med.

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“…The VlsE lipoprotein is located on the surface of the Borrelia spirochetes and is thereby likely to come into contact with antibodies during the infection 23 . However, the full biological function of the VlsE protein is still unknown, but it may, like OspC, serve as a protector to the pathogen in the host defence 24 .…”

Section: Borrelia Proteins and Genes -Function And Expressionmentioning

confidence: 99%

Molecular and serological tools for clinical diagnostics of Lyme borreliosis - can the laboratory analysis be improved?

Lager

2020

Linköping University Medical Dissertations

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Lyme borreliosis (LB) is caused by spirochetes within the Borrelia burgdorferi sensu lato complex and is the most common tick-transmitted disease in the northern hemisphere. The transmission of the spirochetes to humans in Europe is done by the Ixodes ricinus ticks, which can also transmit the relapsing fever species Borrelia miyamotoi. LB may cause clinical manifestations in the skin, in the central nervous system, in joints, and in the heart. Diagnosis of LB is mainly based on the patient´s medical history, self-described symptoms, and clinical signs in combination with the detection of Borrelia-specific antibodies (serological methods). In some cases/issues, detection of Borrelia-specific deoxyribonucleic acid (molecular methods) may be used as a complement to serology. All diagnosed LB infections are treated with antibiotics to prevent disease progression, and most patients fully recover without further sequelae. The overall aims of this thesis were to evaluate molecular and serological tools for laboratory diagnosis of LB, with a special focus on Lyme neuroborreliosis (LNB), and to identify potential improvements. The results presented in this thesis showed that the immunoglobulin (Ig) G assays, currently in use in northern Europe for detection of antibodies in serum, had high diagnostic sensitivity (88 %) together with comparable results both between and within assays. For the IgM assays, the diagnostic sensitivity was lower (59 %) with more heterogeneous results. Small variations in diagnostic performance for IgM and IgG were mainly presented for samples within the borderline zone. These results support the theory that separate testing of IgM antibodies in serum has low diagnostic value. However, simultaneous detection in serum and cerebrospinal fluid (CSF) for both IgM and IgG antibodies was essential for the diagnosis of LNB, at least for certain assays. So far (to our knowledge), no systematic evaluation and optimisation of the pre-analytical handling of CSF samples before molecular testing has been performed. By use of the precipitate concentrated by moderate centrifugation, extraction of total nucleic acid followed by reversetranscription to complementary deoxyribonucleic acid, in combination with the absence of Abstract polymerase chain reaction (PCR) inhibitors, detection of Borrelia garinii, Borrelia afzelii, Borrelia burgdorferi sensu stricto, and B. miyamotoi was possible. These four species are all known to be pathogenic to humans. The results revealed a high analytical sensitivity and specificity for the optimised pre-analytical conditions. The thesis also presents results showing that the real-time PCR protocols currently used in Scandinavia have high analytical sensitivity, specificity, and concordance. This indicates that the low diagnostic sensitivity for detection of Borrelia in CSF was not a result of poorly designed and evaluated PCR protocols, but was possibly due to the low number of spirochetes in the samples. However, to further evaluate the diagnostic performance for detection of Bo...

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“…One of the problems in Lyme disease is the lack of a reliable marker with high sensitivity and specificity for evaluation of the effectiveness of antibiotic therapy administered to patients. A marker that could be used for such a control should appear instantaneously in patient's serum after Borreliella infection, and disappear immediately after successful treatment of the disease [5]. Various studies have shown that IgG anti-C6VlsE antibodies appear relatively quickly in early and late Lyme disease [6, 7, and disappear relatively quickly after antibiotic therapy [8,9].…”

Section: Introductionmentioning

confidence: 99%

Analysis of the concentration of selected serological parameters in patients undergoing antibiotic treatment of Lyme disease and assessment of their potential application in the control of the therapy effectiveness – pilot study

Tokarska-Rodak¹,

Fota-Markowska²,

Pańczuk³

et al. 2021

Ann Agric Environ Med.

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ROLE OF VlsE/C6 ANTIGEN AS A MARKER FOR EARLY LYME BORRELIOSIS DIAGNOSIS AND MONITORING THE EFFECTIVENESS OF ITS TREATMENT

Cited by 5 publications

References 19 publications

Analysis of selected serological parameters in patients with diagnosed Lyme borreliosis and in seropositive patients with no clinical symptoms

Analysis of selected serological parameters in patients with diagnosed Lyme borreliosis and in seropositive patients with no clinical symptoms

Molecular and serological tools for clinical diagnostics of Lyme borreliosis - can the laboratory analysis be improved?

Analysis of the concentration of selected serological parameters in patients undergoing antibiotic treatment of Lyme disease and assessment of their potential application in the control of the therapy effectiveness – pilot study

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