Role of volume-regulated and calcium-activated anion channels in cell volume homeostasis, cancer and drug resistance

Hoffmann, Else K.; Sørensen, Belinda Halling; Sauter, Daniel; Lambert, Ian Henry

doi:10.1080/19336950.2015.1089007

Cited by 43 publications

(48 citation statements)

References 162 publications

(226 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…5). It is known that Na + /K + pump inactivation leads to cell hydration [16], [17], [18]. The obtained results showing that 10 -4 M ouabain, having inhibitory effect on Na + /K + pump, leads to hydration in all three investigated tissues of sham animals (Fig.…”

Section: Discussionmentioning

confidence: 58%

“…From these data it can be concluded, that there is a metabolic mechanism which is responsible for the increase of brain tissue hydration and decrease of heart muscle tissue hydration in 4Hz MV-treated animals. It is known that electrogenic Na + /K + pump has a crucial role in metabolic control of cell hydration [16], [17], [18]. In order to find out the role of Na + /K + pump in realization of 4Hz MV-induced hydration changes in the aforementioned three types of tissues (cortex, cerebellum and heart muscle), in the next series of experiments, the sensitivity of 4Hz MV-induced changes of tissue hydration to 10 -4 M ouabain (inhibiting Na + /K + pump) was studied.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

4Hz mechanical vibration relieves pain through Na+/K+-ATPase α3 isoform-dependent brain tissue dehydration

Musheghyan

Minasyan

Arajyan

et al. 2017

IJBAS

View full text Add to dashboard Cite

In this work the effect of 4Hz 30dB horizontal mechanical vibration (MV) on thermal pain threshold, hydration and [3 H]-ouabain binding in brain and heart muscle tissues of rats was studied. It was revealed that 4Hz MV treatment for 10 minutes increased pain threshold, which was accompanied by brain and heart muscle tissue dehydration. In vitro state, hydration of brain and heart muscle tissues of sham animals was increased, while in 4Hz MV-treated animals the increase of brain tissue hydration was more pronounced and heart muscle tissues were dehydrated. The fact that 4Hz MV treatment also impacted heart muscle tissue hydration indicates that 4Hz MV effect on brain and heart muscle tissues is realized through a common messenger circulating in blood. The incubation of brain and heart muscle tissues in PS containing 10 -4 M and 10 -9 M ouabain led to tissue hydration in sham and 4Hz MV-treated animals. However, tissues of 4Hz MV-treated animals were less hydrated, and this hydration was accompanied by the decrease and increase of membrane receptors' affinity at 10 -4 M and 10 -9 M ouabain concentrations, respectively. Based on the obtained data, it is suggested that pain-relieving effect of 4Hz MV is due to α3 isoform-dependent brain tissue dehydration.

show abstract

Section: Discussionmentioning

confidence: 58%

Section: Resultsmentioning

confidence: 99%

4Hz mechanical vibration relieves pain through Na+/K+-ATPase α3 isoform-dependent brain tissue dehydration

Musheghyan

Minasyan

Arajyan

et al. 2017

IJBAS

View full text Add to dashboard Cite

show abstract

“…It is known that in living cells intracellular osmotic pressure exceeds the extracellular one. As cell membrane is highly permeable for water molecules, the metabolically driven water efflux balances water uptake by cell [15], [16], [34]. In our previous study it has been shown that water influx through membrane has activation effect on Na + and Ca 2+ inward currents, while water efflux from the cell has inactivation effect on them [33], [35].…”

Section: Discussionmentioning

confidence: 99%

“…However, the nature of the messenger through which NT MW modulates the structural changes in cell aqua medium and metabolically controls brain tissue hydration has not been elucidated yet. Based on the facts that in living cells: a) water, being a common medium for intracellular reactions, determines the metabolic activity of cells, b) intracellular osmotic pressure exceeds the extracellular one, c) cell membrane has high permeability for water [15], [16] and d) water molecule dissociation and reactive oxygen species (ROS) are increased after NT MW radiation, it is suggested that water molecules and their dissociation products are primary messengers for NT MW effect on cell metabolism. To check this hypothesis the effects of intraperitoneal (IP) injection of physiological solution (PS) preliminarily treated by NT MW on tissue dehydration, [ 3 H]-ouabain binding with membrane, 45 Ca 2+ uptake and intracellular content of cyclic nucleotides in rats' brain cortex have been studied.…”

Section: Introductionmentioning

confidence: 99%

Non-thermal microwave radiation-induced brain tissue dehydration as a potential factor for brain functional impairment

Nikoghosyan

Heqimyan

Ayrapetyan

2016

IJBAS

View full text Add to dashboard Cite

Based on our previous finding that metabolically controlled cell hydration is sensitive to the changes of physicochemical properties of cell aqua medium, which take place upon the effect of weak physical signals, including microwaves with non-thermal intensity (NT MW), it has been hypothesized that cell aqua medium serves as a primary target for NT MW effect on brain tissue. To elucidate the nature of the metabolic pathway through which the effect of NT MW-induced changes of physicochemical properties of cell aqua medium modulate cell hydration, the effects of intraperitoneal (IP) injection of PS treated by NT MW on brain tissue hydration, 45 Ca 2+ uptake, ] i), it is considered as a potential factor leading to the brain functional impairment, especially when brain metabolic activity is depressed (e.g. during aging).

show abstract

“…TMC8 also inhibits volume-activated Cl -currents, suggesting that TMC8 may be associated with volume regulated anion channel (VRAC) (7,25). VRAC is a volume regulator, and also affects several other functions, such as apoptosis, cell proliferation and migration (26). In addition, TMC8 may promote TNF-α-induced apoptosis by binding TNF receptor type 1-associated DEATH domain protein through the TMC-containing region (8).…”

Section: Discussionmentioning

confidence: 99%

Transmembrane channel‑like protein 8 as a potential biomarker for poor prognosis of hepatocellular carcinoma

Ding

et al. 2017

mol clin onc

View full text Add to dashboard Cite

Abstract. Hepatocellular carcinoma (HCC) is an aggressive malignant tumor and the third leading cause of cancer-related mortality worldwide. Transmembrane channel-like protein 8 (TMC8) is reported to play a major role in several aspects of human pathophysiology, such as ion channel permeability, human papillomavirus infection and skin cancer; however, its role in HCC has not been fully elucidated. The aim of the present study was to investigate the expression levels of TMC8 in 146 pairs of liver cancer samples and adjacent non-tumorous samples using immunohistochemistry. Reverse transcription-quantitative polymerase chain reaction analysis was used to confirm the results. The association between TMC8 expression and clinicopathological characteristics, including overall survival, was analyzed. The results indicated that the expression of TMC8 was significantly upregulated in HCC tissues and associated with metastasis and hepatitis B virus infection. According to the analysis of the overall survival using Cox proportional hazard regression model, higher expression of TMC8 was associated with a poorer prognosis and the overexpression of TMC8 was an independent risk factor for HCC. By contrast, HBsAg did not significantly affect the survival of HCC patients. These results suggest that the overexpression of TMC8 in HCC predicts poor prognosis and may be a potential biomarker for this type of cancer.

show abstract

Role of volume-regulated and calcium-activated anion channels in cell volume homeostasis, cancer and drug resistance

Cited by 43 publications

References 162 publications

4Hz mechanical vibration relieves pain through Na+/K+-ATPase α3 isoform-dependent brain tissue dehydration

4Hz mechanical vibration relieves pain through Na+/K+-ATPase α3 isoform-dependent brain tissue dehydration

Non-thermal microwave radiation-induced brain tissue dehydration as a potential factor for brain functional impairment

Transmembrane channel‑like protein 8 as a potential biomarker for poor prognosis of hepatocellular carcinoma

Contact Info

Product

Resources

About