Role of yes‐associated protein in interleukin‐13 induced nasal remodeling of chronic rhinosinusitis with nasal polyps

Tian, Yuan; Zheng, Rui; Liu, Jing; Tan, Kai Sen; Huang, Zhi-Qun; Zhou, Xiang-Min; Zi, Xiaoxue; Qiu, Huijun; Wang, Xinyue; Wang, Wei-Hao; Deng, Huiyi; Chen, Yubin; Kong, Weihao; Wu, Qingwu; Huang, Ying; Ong, Hsiao Hui; Huang, Xianbo; Zhuang-gui, Chen; Wang, Deyun; Yang, Qintai

doi:10.1111/all.14699

Cited by 15 publications

(17 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For goblet cells differentiation, no significant association of nuclear active YAP was found in Grade II NIP, as goblet cells levels are very low in NIP. However, in our recently published paper (Yuan et al, 2020), YAP may play a double role in repressing ciliated cell differentiation while promoting goblet cell differentiation, which was not seen in NIP. The difference in the levels of nuclear active YAP may lead to the discrepancies between NP and NIP observation, which remained to be further explored if there are other pathways influencing the goblet cell differentiation.…”

Section: Discussionmentioning

confidence: 78%

“…Human nasal epithelial cells (hNECs) were developed from primary human nasal epithelial stem/progenitor cells (hNESPCs) (Li et al, 2014;Liu et al, 2018;Yuan et al, 2020), obtained from fresh healthy inferior turbinate mucosa. The hNESPCs were transplanted to an air-liquid interface (ALI) system to form a pseudostratified layer within 4 weeks.…”

Section: Human Nasal Epithelial Cells Culture and Il-13 Treatmentmentioning

confidence: 99%

“…The hNESPCs were transplanted to an air-liquid interface (ALI) system to form a pseudostratified layer within 4 weeks. A detailed description of the hNEC culture method used is found in our previously published papers (Li et al, 2014;Liu et al, 2018;Yuan et al, 2020). To establish a remodeling condition in vitro, IL-13 (10 ng/mL, R&D System, Minneapolis, MN, United States) was added to the basal cell culture medium of the hNECs during their entire duration of differentiation.…”

Section: Human Nasal Epithelial Cells Culture and Il-13 Treatmentmentioning

confidence: 99%

See 2 more Smart Citations

Abnormal Expression of YAP Is Associated With Proliferation, Differentiation, Neutrophil Infiltration, and Adverse Outcome in Patients With Nasal Inverted Papilloma

Tian

Zheng

Zhou

et al. 2021

Front. Cell Dev. Biol.

Self Cite

View full text Add to dashboard Cite

BackgroundNasal inverted papilloma (NIP) is a common benign tumor. Yes-associated protein (YAP) is the core effector molecule of the Hippo pathway, which regulates the proliferation and differentiation of airway epithelium. While its role in proliferation may be connected to NIP formation, no definitive association has been made between them.MethodsWe compared the difference of YAP expression and proliferation level between the control inferior turbinate, NP (nasal polyps), and NIP groups. In addition, we further used PCR, immunofluorescence, and immunohistochemistry to investigate YAP’s role in the proliferation and differentiation of the nasal epithelium and inflammatory cell infiltration, correlating them with different grades of epithelial remodeling. We further used an IL-13 remodeling condition to investigate YAP’s role in differentiation in an in vitro air-liquid interface (ALI) human nasal epithelial cell (hNECs) model. Finally, we also explored the correlation between YAP expression and clinical indicators of NIP.ResultsThe expression of YAP/active YAP in the NIP group was significantly higher than that in the NP group and control group. Moreover, within the NIP group, the higher grade of epithelial remodeling was associated with higher YAP induced proliferation, leading to reduced ciliated cells and goblet cells. The finding was further verified using an IL-13 remodeling condition in differentiating ALI hNECs. Furthermore, YAP expression was positively correlated with proliferation and neutrophil infiltration in NIP. YAP expression was also significantly increased in NIP patients with adverse outcomes.ConclusionAbnormal expression of YAP/active YAP is associated with proliferation, differentiation, neutrophil infiltration, and adverse outcome in NIP and may present a novel target for diagnosis and intervention in NIP.

show abstract

Section: Discussionmentioning

confidence: 78%

Section: Human Nasal Epithelial Cells Culture and Il-13 Treatmentmentioning

confidence: 99%

Section: Human Nasal Epithelial Cells Culture and Il-13 Treatmentmentioning

confidence: 99%

See 1 more Smart Citation

Abnormal Expression of YAP Is Associated With Proliferation, Differentiation, Neutrophil Infiltration, and Adverse Outcome in Patients With Nasal Inverted Papilloma

Tian

Zheng

Zhou

et al. 2021

Front. Cell Dev. Biol.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Previous studies have confirmed that IL13 participates in the destruction of epithelial barrier and promotes T H 2 inflammation [39,40]. Furthermore, p63 has been regarded as a direct effector of IL13-induced T H 2 inflammation [41].…”

Section: Discussionmentioning

confidence: 88%

Upregulation of Basonuclin1 Is Associated with p63-Involved Epithelial Barrier Impairment and Type-2 Helper T-cell Inflammation in Chronic Rhinosinusitis with Nasal Polyps

Gao

Jiao

et al. 2021

Int Arch Allergy Immunol

View full text Add to dashboard Cite

Background: Tumor protein p63 has been shown to be important for epithelial dysfunction, including epithelial barrier defects and mucosal inflammation, in the development of chronic rhinosinusitis with nasal polyps (CRSwNP). Basonuclin1 (BNC1), an epithelial-specific transcriptional factor, is a direct downstream target of p63 and thus might be involved in the pathogenesis of CRSwNP. Objective: We sought to investigate whether BNC1 was associated with p63-mediated epithelial barrier defects and nasal mucosal inflammation in CRSwNP. Methods: Nasal tissue biopsies were obtained from 91 patients to CRSwNP, 49 chronic rhinosinusitis without nasal polyps (CRSsNP) patients, and 28 control subjects. Immunohistochemistry and immunofluorescence staining were used to determine the distribution of BNC1 in tissues and localization in cells, respectively. Quantitative PCR was performed to detect the expression levels of BNC1, TP63, epithelial barrier proteins, and type-2 helper T-cell inflammation-related genes. Results: BNC1 mRNA expression was significantly elevated in the tissues in CRSwNP patients compared with CRSsNP (1.96-fold, p = 0.0003) and control groups (2.40-fold, p < 0.0001). BNC1 staining was strongly positive in the nasal epithelium and co-localized with p63-positive epithelial cells. The expression of BNC1 mRNA was strongly correlated with TP63 mRNA level both in tissue biopsies (r = 0.78, p < 0.0001) and epithelial scrapings (r = 0.97, p < 0.0001). BNC1 expression was also positively correlated with epithelial barrier protein genes (CDH1, CLDN1, CLDN4, TJP1, and TJP2) and epithelial genes involved in TH2 inflammation (IL33, CCL26, CLC, and ALOX15). Conclusions: Overexpression of BNC1 may be associated with increased expression of TP63, and possibly contribute to the epithelial barrier defects and TH2 inflammation in CRSwNP.

show abstract

“…This process could be reversed by using a YAP inhibitor. 100 The different CRS endotypes are characterized by different types of cellular and pathological features. Recently, a specific CD38 high CD117 high mast cell population has been identified in CRSwNP patients that expands during an inflammatory response.…”

Section: Biomarkers In Chronic Rhinosinusitismentioning

confidence: 99%

Advances and highlights in biomarkers of allergic diseases

Öğülür

Pat

Ardicli

et al. 2021

Allergy

122

View full text Add to dashboard Cite

During the past years, there has been a global outbreak of allergic diseases, presenting a considerable medical and socioeconomical burden. A large fraction of allergic diseases is characterized by a type 2 immune response involving Th2 cells, type 2 innate lymphoid cells, eosinophils, mast cells, and M2 macrophages. Biomarkers are valuable parameters for precision medicine as they provide information on the disease endotypes, clusters, precision diagnoses, identification of therapeutic targets, and monitoring of treatment efficacies. The availability of powerful omics technologies, How to cite this article: Ogulur I, Pat Y, Ardicli O, et al. Advances and highlights in biomarkers of allergic diseases.

show abstract

Role of yes‐associated protein in interleukin‐13 induced nasal remodeling of chronic rhinosinusitis with nasal polyps

Cited by 15 publications

References 9 publications

Abnormal Expression of YAP Is Associated With Proliferation, Differentiation, Neutrophil Infiltration, and Adverse Outcome in Patients With Nasal Inverted Papilloma

Abnormal Expression of YAP Is Associated With Proliferation, Differentiation, Neutrophil Infiltration, and Adverse Outcome in Patients With Nasal Inverted Papilloma

Upregulation of Basonuclin1 Is Associated with p63-Involved Epithelial Barrier Impairment and Type-2 Helper T-cell Inflammation in Chronic Rhinosinusitis with Nasal Polyps

Advances and highlights in biomarkers of allergic diseases

Contact Info

Product

Resources

About