Role of zinc in cellular zinc trafficking and mineralization in a murine osteoblast-like cell line

Nagata, Masashi; Lönnerdal, Bo

doi:10.1016/j.jnutbio.2010.01.003

Cited by 93 publications

(48 citation statements)

References 38 publications

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“…This result is similar to those from studies in mammals, as it has been demonstrated that MT plays a protective role in mercury-induced toxicity in bone (Jin et al, 2002). As it has been reported that osteoblasts express MT and protect from heavy metal (Angle et al, 1990;Nagata and Lönnerdal, 2011), the activation of MT in osteoblasts may be involved in resistance to mercury. In mammals, the influence of mercury on bone metabolism has been studied mainly by in vivo experiments and investigated in bone formation or osteoblastic activity (Yonaga et al, 1985;Jin et al, 2002).…”

Section: Discussionsupporting

confidence: 90%

Effects of Inorganic Mercury and Methylmercury on Osteoclasts and Osteoblasts in the Scales of the Marine Teleost as a Model System of Bone

et al. 2014

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To evaluate the effects of inorganic mercury (InHg) and methylmercury (MeHg) on bone metabolism in a marine teleost, the activity of tartrate-resistant acid phosphatase (TRAP) and alkaline phosphatase (ALP) as indicators of such activity in osteoclasts and osteoblasts, respectively, were examined in scales of nibbler fish (Girella punctata). We found several lines of scales with nearly the same TRAP and ALP activity levels. Using these scales, we evaluated the influence of InHg and MeHg. TRAP activity in the scales treated with InHg (10 -5 and 10 -4 M) and MeHg (10 -6 to 10 -4 M) during 6 hrs of incubation decreased significantly. In contrast, ALP activity decreased after exposure to InHg (10 -5 and 10 -4 M) and MeHg (10 -6 to 10 -4 M) for 18 and 36 hrs, although its activity did not change after 6 hrs of incubation. As in enzyme activity 6 hrs after incubation, mRNA expression of TRAP (osteoclastic marker) decreased significantly with InHg and MeHg treatment, while that of collagen (osteoblastic marker) did not change significantly. At 6 hrs after incubation, the mRNA expression of metallothionein, which is a metal-binding protein in osteoblasts, was significantly increased following treatment with InHg or MeHg, suggesting that it may be involved in the protection of osteoblasts against mercury exposure up to 6 hrs after incubation. To our knowledge, this is the first report of the effects of mercury on osteoclasts and osteoblasts using marine teleost scale as a model system of bone.

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Section: Discussionsupporting

confidence: 90%

Effects of Inorganic Mercury and Methylmercury on Osteoclasts and Osteoblasts in the Scales of the Marine Teleost as a Model System of Bone

et al. 2014

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“…Zinc supplementation has also been shown to favorably modulate bone turnover by stimulating osteoblast bone formation while inhibiting osteoclast differentiation. These effects combine to increase bone strength [309][310][311][312][313]. Zinc supplementation also alleviates toxic effects of other metals, particularly cadmium [64,314], and it may prevent or treat several types of pathological bone loss [315].…”

Section: Zincmentioning

confidence: 99%

Effects of thirty elements on bone metabolism

Dermience

Lognay

Mathieu³

et al. 2015

Journal of Trace Elements in Medicine and Biology

207

152

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“…Collagen I, osteocalcin (OC), ALP, and bone sialoprotein (BSP) expressions are improved by Zn. Zn may play a major role in osteoblast mineralization through intra-and extra-cellular Zn movements involving Zn storage proteins and transporters (Nagata and Lonnerdal, 2011). As for osteoblasts, Zn reduces interleukin-6 (IL-6), a potent bone resorptive agent which increases osteoclast formation and stimulates osteoclast activity to resorb bone at the bone implant interface (Hatakeyama et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

Effects of zinc-substituted nano-hydroxyapatite coatings on bone integration with implant surfaces

Zhao

Dong

Jiang

et al. 2013

J. Zhejiang Univ. Sci. B

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Abstract:Objective: The purpose of this study was to investigate the effects of a zinc-substituted nano-hydroxyapatite (Zn-HA) coating, applied by an electrochemical process, on implant osseointegraton in a rabbit model. Methods: A Zn-HA coating or an HA coating was deposited using an electrochemical process. Surface morphology was examined using field-emission scanning electron microscopy. The crystal structure and chemical composition of the coatings were examined using an X-ray diffractometer (XRD) and Fourier transform infrared spectroscopy (FTIR). A total of 78 implants were inserted into femurs and tibias of rabbits. After two, four, and eight weeks, femurs and tibias were retrieved and prepared for histomorphometric evaluation and removal torque (RTQ) tests. Results: Rod-like HA crystals appeared on both implant surfaces. The dimensions of the Zn-HA crystals seemed to be smaller than those of HA. XRD patterns showed that the peaks of both coatings matched well with standard HA patterns. FTIR spectra showed that both coatings consisted of HA crystals. The Zn-HA coating significantly improved the bone area within all threads after four and eight weeks (P<0.05), the bone to implant contact (BIC) at four weeks (P<0.05), and RTQ values after four and eight weeks (P<0.05). Conclusions: The study showed that an electrochemically deposited Zn-HA coating has potential for improving bone integration with an implant surface.

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Role of zinc in cellular zinc trafficking and mineralization in a murine osteoblast-like cell line

Cited by 93 publications

References 38 publications

Effects of Inorganic Mercury and Methylmercury on Osteoclasts and Osteoblasts in the Scales of the Marine Teleost as a Model System of Bone

Effects of Inorganic Mercury and Methylmercury on Osteoclasts and Osteoblasts in the Scales of the Marine Teleost as a Model System of Bone

Effects of thirty elements on bone metabolism

Effects of zinc-substituted nano-hydroxyapatite coatings on bone integration with implant surfaces

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