Role of β-endorphin in the regulation of proinflammatory cytokine production by peripheral blood monocytes in vitro

Abstract

beta-Endorphin activated interleukin-1beta production in the culture of unfractionated cells with lipopolysaccharide, but had no effect on the synthesis of tumor necrosis factor-alpha and interleukin-6. This peptide produced a slight stimulatory effect on spontaneous production of interleukin-1beta by cultured leukocytes. Opioid receptor blockade with naloxone and naltrindole did not abolish the stimulatory effect of beta-endorphin on interleukin-1beta production. beta-Endorphin did not modulate the synthesis of interleukin-1beta, tumor necrosis factor-alpha, and interleukin-6 in a purified fraction of monocytes.

Inhibitory effects of dynorphin 3-14 on the lipopolysaccharide-induced toll-like receptor 4 signalling pathway

“…The differential effects seen on cytokine release in this study are consistent with evidence from a previous study, showing that β-endorphin and naltrindole elevated IL-1β production and suppressed TNF-α level in LPS-stimulated peripheral blood monocytes [35]. The differential secretion of TNF-α and IL-1β following LPS induction in isolated mouse macrophages further describes the complexity of cytokine modulation by immune cells [36].…”

Effect of Endomorphins-1, 2 on Functional Activity of Neutrophils and Peripheral Blood Monocytes In Vitro

“…In this regard, the effects of endomorphins are much closer to the effects of β-endorphin, another endogenous peptide ligand of μ receptors, which also has a multidirectional effect on a wide range of immune responses in vitro and in vivo. We have previously shown in vitro a similar effect of β-endorphin, which inhibits the production of ROSs and stimulates the secretion of IL-1β [12,13]. At the same time, in vivo, β-endorphin stimulates zymosaninduced secretion of ROSs and inhibits the spontaneous production of IL-1β and IL-10 by murine peritoneal macrophages [14].…”

“…In addition, the direction of the action of endomorphins, like that of other regulatory peptides, depends on the concentration, composition of the cell fraction, and an additional activation signal. It should be noted that endomorphins modulate ROS production both in mixed cell suspension and in the neutrophil fraction, whereas the modulation of cytokine production takes place only in the mononuclear fraction and is absent in the fraction of purified monocytes, which suggests the need for intercellular interaction for opioid-mediated regulation of cytokine produc- but not by purified monocytes [13,16]. Comparative analysis of the action of the two peptides in general showed their unidirectional effect on the studied parameters; however, it should be noted that the spectrum of effects exerted by endomorphin-2 was somewhat broader and more pronounced.…”

Effect of opioid peptides on oxygen-dependent microbicidal activity of peripheral blood neutrophils

“…In the leukocyte suspension, opioids stimulate the in vitro production of IL 1β, an important proinflammatory factor markedly activat ing the functional activity of granulocytes, by leuko cytes [14]. At the same time, the is no production of IL 1β by purified monocytes in the presence of β endorphin [15].…”