Abstract. Ourrad O, Ahmed DSA, Sadou SA, Bouzrarf K, Belqat B. 2022. Diversity of flies (Diptera: Brachycera) in breeding farms in the Kabylia region (North-central Algeria), and identification of some myiaseginic species. Biodiversitas 23: 2276-2284. This work provides the first checklist of the flies (Insecta: Diptera) present in breeding farms located in the south Mediterranean region of Kabylia, Algeria. A new inventory of 26 species (631 individuals) of flies belonging to 15 genera and 8 families was presented from five sites with different altitudes (245 m - 1041 m) in the Kabylia region. Of them, eight species (Calliphora vicina, C. vomitoria, Lucilia sericata, L. ampullacea, Sarcophaga africa, S. carnaria, Musca domestica, and Fannia canicularis) are epidemiological, pathogenic agents of various animal and human myiasis. The fly fauna was found at varying frequencies in the five sites located at different altitudes. Besides, a negative correlation was observed between the altitude of the farms and fly distribution. The results of the PCA and FCA analysis offered better discrimination of the fly species according to the variation of the environmental parameters.