Roles for Ctk1 and Spt6 in Regulating the Different Methylation States of Histone H3 Lysine 36

Youdell, Michael; Kizer, Kelby O.; Kisseleva-Romanova, Elena; Fuchs, Stephen M.; Duro, Eris; Strahl, Brian D.; Mellor, Jane

doi:10.1128/mcb.00001-08

Cited by 150 publications

(214 citation statements)

References 49 publications

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“…Protein Levels Are Regulated in Vivo-We previously noted that Set2 protein was lost in CTK1 delete cells (ctk1⌬), and this was not the result of changes in SET2 gene expression (14,25). These data, combined with our observation that Set2 protein levels correlate with RNAPII CTD phosphorylation levels, prompted us to explore the regulation of the Set2 protein further.…”

Section: Set2mentioning

confidence: 74%

“…We recently reported that deletion of the kinase responsible for Ser 2 phosphorylation of the CTD, Ctk1, results not only in loss of H3K36me but also decreased Set2 protein levels (14). This finding suggested a novel mechanism for regulating histone methyltransferase activity.…”

Section: Methylation Of Lysine 36 On Histone H3 (H3k36) Is Catalyzed mentioning

confidence: 95%

“…Strains created for this study were constructed using heterologous gene replacement (33,34) or through classical yeast mating in combination with tetrad dissection or high throughput strain construction techniques (35). Set2 expression plasmids (14,21) were transformed using the lithium chloride technique (36), and shuffle strains (where important genes were genomically deleted but carried on a uracil-selectable plasmid) were maintained on selective media until the time of the appropriate experiment, and plasmid loss was instigated by plating on media containing 5-fluoroorotic acid (5-FOA) (37).…”

Section: Methodsmentioning

confidence: 99%

“…Subsequent studies revealed that H3K36me2 is the preferred substrate for Rpd3(S) binding (12)(13)(14) and that a primary function for H3K36 methylation is to recruit the repressive activity of Rpd3(S) to genes to "reset" or reestablish chromatin structure between multiple rounds of transcription (15)(16)(17). Such resetting, or at least maintaining a more compact chromatin structure within gene bodies, also protects genes from spurious transcription from improper initiation sites (cryptic transcription) (15)(16)(17)(18).…”

Section: Methylation Of Lysine 36 On Histone H3 (H3k36) Is Catalyzed mentioning

confidence: 99%

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RNA Polymerase II Carboxyl-terminal Domain Phosphorylation Regulates Protein Stability of the Set2 Methyltransferase and Histone H3 Di- and Trimethylation at Lysine 36

Fuchs

Kizer

Braberg

et al. 2012

Journal of Biological Chemistry

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Background: Set2 catalyzes mono-, di-, and trimethylation of lysine 36 on histone H3 (H3K36). Results: Phosphorylation of the C-terminal domain of RNA polymerase II (RNAPII CTD) regulates Set2 protein levels and H3K36 methylation. Conclusion: Set2 protein is regulated to maintain balanced levels of H3K36 methylation. Significance: These results suggest that different methylation states perform distinct biological functions.

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Section: Set2mentioning

confidence: 74%

Section: Methylation Of Lysine 36 On Histone H3 (H3k36) Is Catalyzed mentioning

confidence: 95%

Section: Methodsmentioning

confidence: 99%

Section: Methylation Of Lysine 36 On Histone H3 (H3k36) Is Catalyzed mentioning

confidence: 99%

See 2 more Smart Citations

RNA Polymerase II Carboxyl-terminal Domain Phosphorylation Regulates Protein Stability of the Set2 Methyltransferase and Histone H3 Di- and Trimethylation at Lysine 36

Fuchs

Kizer

Braberg

et al. 2012

Journal of Biological Chemistry

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“…To investigate the importance of the Spt6 SH2 domain in vivo, we carried out Affymetrix gene expression profiling (Affymetrix GeneChip Yeast Genome 2.0) with a yeast strain lacking the C-terminal region of Spt6 that includes the SH2 domain 30 (strain spt6ΔC-TAP, Materials and Methods). This mutant strain shows a significant slow-growth phenotype (Supplementary Data Fig.…”

Section: Requirement Of the Spt6 Sh2 Domain In Vivomentioning

confidence: 99%

Structure and in Vivo Requirement of the Yeast Spt6 SH2 Domain

Dengl

Mayer

Sun

et al. 2009

Journal of Molecular Biology

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During transcription elongation through chromatin, the Ser2-phosphorylated C-terminal repeat domain of RNA polymerase II binds the C-terminal Src homology 2 (SH2) domain of the nucleosome re-assembly factor Spt6. This SH2 domain is unusual in its specificity to bind phosphoserine, rather than phosphotyrosine and because it is the only SH2 domain in the yeast genome. Here, we report the high-resolution crystal structure of the SH2 domain from Candida glabrata Spt6. The structure combines features from both structural subfamilies of SH2 domains, suggesting it resembles a common ancestor of all SH2 domains. Two conserved surface pockets deviate from those of canonical SH2 domains, and may explain the unusual phosphoserine specificity. Differential gene expression analysis reveals that the SH2 domain is required for normal expression of a subset of yeast genes, and is consistent with multiple functions of Spt6 in chromatin transcription.

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SPT6 interacts with NSD2 and facilitates interferon‐induced transcription

et al. 2018

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The role of the histone chaperone SPT6 in mammalian cells is not fully understood. Here, we investigated the involvement of SPT6 in type I interferon (IFN)-induced transcription in murine fibroblasts. In RNA-seq analysis, Spt6 siRNA attenuates about half of ~ 200 IFN-stimulated genes (ISGs), while not affecting housekeeping genes. ISGs with high mRNA induction are more susceptible to Spt6 siRNA than those with lower levels of induction. ChIP analysis shows that SPT6 is recruited to highly inducible, Spt6 siRNA-sensitive ISGs, but not to other siRNA-insensitive ISGs. Furthermore, SPT6 recruitment is abrogated in cells lacking the histone methyltransferase NSD2. In co-IP experiments, SPT6 interacts with NSD2. In summary, SPT6 facilitates IFN-induced transcription, highlighting its critical role in gene activation.

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Roles for Ctk1 and Spt6 in Regulating the Different Methylation States of Histone H3 Lysine 36

Cited by 150 publications

References 49 publications

RNA Polymerase II Carboxyl-terminal Domain Phosphorylation Regulates Protein Stability of the Set2 Methyltransferase and Histone H3 Di- and Trimethylation at Lysine 36

RNA Polymerase II Carboxyl-terminal Domain Phosphorylation Regulates Protein Stability of the Set2 Methyltransferase and Histone H3 Di- and Trimethylation at Lysine 36

Structure and in Vivo Requirement of the Yeast Spt6 SH2 Domain

SPT6 interacts with NSD2 and facilitates interferon‐induced transcription

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