Roles for the prostaglandins in the regulation of anterior pituitary secretion

Hedge, George A.

doi:10.1016/0024-3205(77)90124-2

Cited by 102 publications

(51 citation statements)

References 82 publications

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“…investigated for many years (Hedge, 1977;Labrie et al, 1982). Prostaglandins stimulate ACTH secretion but this e ect is now mainly considered to be via a central action to stimulate CRF secretion from the hypothalamus (De Wied et al, 1969;Hedge & Hanson, 1972;Peng et al, 1970;Thompson & Hedge, 1978).…”

Section: Figurementioning

confidence: 99%

A direct inhibitory action of prostaglandins upon ACTH secretion at the late stages of the secretory pathway of AtT‐20 cells

Wilson

Guild

2002

British J Pharmacology

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The mouse AtT‐20/D16‐16 anterior pituitary tumour cell line was used as a model system for the study of the effects of prostaglandins upon the late stages of the adrenocorticotrophin (ACTH) secretory pathway. Calcium (1 nM – 100 μM), guanosine‐5′‐O‐(3‐thiotriphosphate) (GTP‐γ‐S) (1 – 100 μM) and mastoparan (1 and 10 μM) all stimulated ACTH secretion from permeabilized AtT‐20 cells in a concentration‐dependent manner. GTP‐γ‐S and mastoparan stimulated ACTH secretion from permeabilized cells in the absence of calcium. Co‐incubation with prostaglandins E1 and E2 (PGE1, PGE2) (10 μM) but not prostaglandin F2α (PGF2α) (10 μM) significantly inhibited calcium‐, GTP‐γ‐S and mastoparan‐evoked secretion by 30 – 50%. The effects of PGE1 and PGE2 upon GTP‐γ‐S (100 μM)‐, calcium (10 μM)‐ and mastoparan (10 μM)‐evoked secretion were concentration‐dependent. PGE1 significantly inhibited GTP‐γ‐S‐ and calcium‐evoked secretion at concentrations of PGE1 above 1 μM but mastoparan‐evoked secretion only at the highest concentration of PGE1 investigated (10 μM). PGE2 was much more potent than PGE1 and significantly inhibited GTP‐γ‐S‐ and calcium‐evoked secretion at 10 nM and above and mastoparan‐evoked secretion above 1 μM. The inhibitory effects of PGE1 and PGE2 upon calcium‐, GTP‐γ‐S‐ and mastoparan‐stimulated ACTH secretion from permeabilized cells were pertussis toxin (PTX) sensitive. In intact cells PGE1, PGE2 and PGF2α (1 nM – 10 μM) acting singly had little or no effect upon ACTH secretion. However, only PGE2 (1 nM – 10 μM) significantly inhibited corticotrophin‐releasing factor‐41 (CRF‐41) (100 nM)‐evoked secretion in a concentration dependent manner. The present study finds that prostaglandins of the E series exert an inhibitory action, via a pertussis toxin‐sensitive GTP‐binding (G)‐protein, in the late stages of the ACTH secretory pathway distal to the G‐exocytosis (Ge)/calcium point of control. British Journal of Pharmacology (2002) 135, 1851–1858; doi:10.1038/sj.bjp.0704652

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Section: Figurementioning

confidence: 99%

A direct inhibitory action of prostaglandins upon ACTH secretion at the late stages of the secretory pathway of AtT‐20 cells

Wilson

Guild

2002

British J Pharmacology

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“…Introduction. L'administration de prostaglandines FZ!X à des vaches (Labussière et al, 1982) ou à des brebis (Labussière et al, 1983) (Hedge, 1977) ou le GRH (Drouin et Labrie, 1976). Les (Signoret, 1985 …”

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Effets des prostaglandines PGF_2α sur l'éjection du lait de la brebis. Conséquences de l'ovariectomie accompagnée ou non d'une complémentation œstroprogestative

Labussière¹,

Ngui²,

Combaud³

1986

Reprod. Nutr. Dévelop.

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“…In particular, these ubiquitous fatty acids have recently been shown to affect the secretion of each of the hormones of the anterior pituitary gland (1,2). In some instances, the effects of the PGs are direct ones in that they affect the pituitary itself.…”

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confidence: 99%

Pharmacological studies of the involvement of hypothalamic prostaglandins in the regulation of thyrotropin secretion.

Wright¹,

Hedge²

1981

Environ Health Perspect

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A case is made for the involvement of pituitary prostaglandins (PGs) in the regulation of thyrotropin (TSH) secretion by citing recent evidence that TSH release in vivo and in vitro is enhanced by treatment with exogenous PGs and is inhibited by drugs (e.g., indomethacin) that block PG synthesis. Pharmacological studies were then performed to test the hypothesis that hypothalamic PGs also affect TSH secretion indirectly via the appropriate hypothalamic hormones that regulate pituitary secretion. The inhibition of thyroidectomy-induced TSH secretion was used as an endpoint in choosing the best of several drugs purported to inhibit PG synthesis. The established effectiveness of indomethacin and aspirin were used for reference in testing the following drugs: naproxen, mefenamic acid, tranylcypromine, and phenelzine. Only naproxen was found to be effective, but since it was no more potent than indomethacin, the latter drug was used for subsequent work. Indomethacin was stereotaxically implanted into several hypothalamic regions known to regulate TSH secretion, and sequential plasma samples were analyzed for TSH by radioimmunoassay. Bilateral implants of indomethacin in the anterior hypothalamic area increased TSH secretion throughout the 72 hr period of study. Sham inplants at this site and indomethacin implants in other nearby sites were ineffective. These findings suggest that endogenous PGs play an inhibitory role in the hypothalamic regulation of pituitary secretion.Over the past couple of decades it has become well recognized that the prostaglandins (PGs) can exert a wide variety of endocrine and metabolic effects. In particular, these ubiquitous fatty acids have recently been shown to affect the secretion of each of the hormones of the anterior pituitary gland (1, 2). In some instances, the effects of the PGs are direct ones in that they affect the pituitary itself. In contrast, others of the known effects are indirect in that they are exerted at the hypothalamus on factors which in turn alter pituitary secretion. Figure 1. In these experiments, various PGs and TRH (or appropriate vehicles) were infused sequentially directly into the anterior pituitaries of pentobarbital-anesthetized female rats, and plasma samples were assayed for TSH by radioimmunoassay. 83

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Roles for the prostaglandins in the regulation of anterior pituitary secretion

Cited by 102 publications

References 82 publications

A direct inhibitory action of prostaglandins upon ACTH secretion at the late stages of the secretory pathway of AtT‐20 cells

A direct inhibitory action of prostaglandins upon ACTH secretion at the late stages of the secretory pathway of AtT‐20 cells

Effets des prostaglandines PGF_2α sur l'éjection du lait de la brebis. Conséquences de l'ovariectomie accompagnée ou non d'une complémentation œstroprogestative

Pharmacological studies of the involvement of hypothalamic prostaglandins in the regulation of thyrotropin secretion.

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Roles for the prostaglandins in the regulation of anterior pituitary secretion

Cited by 102 publications

References 82 publications

A direct inhibitory action of prostaglandins upon ACTH secretion at the late stages of the secretory pathway of AtT‐20 cells

A direct inhibitory action of prostaglandins upon ACTH secretion at the late stages of the secretory pathway of AtT‐20 cells

Effets des prostaglandines PGF2α sur l'éjection du lait de la brebis. Conséquences de l'ovariectomie accompagnée ou non d'une complémentation œstroprogestative

Pharmacological studies of the involvement of hypothalamic prostaglandins in the regulation of thyrotropin secretion.

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Resources

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Effets des prostaglandines PGF_2α sur l'éjection du lait de la brebis. Conséquences de l'ovariectomie accompagnée ou non d'une complémentation œstroprogestative