Roles of ATR1 paralogs YMR279c and YOR378w in boron stress tolerance

Bozdag, G. Ozan; Uluışık, İrem; Gülcüler, Gülce Sıla; Karakaya, Hüseyin Çağlar; Koç, Ahmet

doi:10.1016/j.bbrc.2011.05.080

Cited by 13 publications

(9 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although the genes comprised in these two lineages do not share common neighbours, this does not exclude the existence of a common evolutionary origin rooting deep in the Fungi phylogenetic tree. The close phylogenetic relationship of ORF YMR279C with the ATR1 gene and the fact that its constitutive expression confers resistance to boron in yeast cells through the decrease of the intracellular levels of this element [24], is consistent with the hypothesis that ORF YMR279C is a boron extrusion pump acting as a back-up of the Atr1p transporter [24]. The proteins belonging to cluster E present in the genomes of the pre-WGD yeast species are more similar to Atr1p than to the ORF YMR279C encoded protein.…”

Section: Discussionmentioning

confidence: 99%

“…Later, a screen for yeast genes conferring resistance to boron revealed that the plasma membrane Atr1p was the main exporter for this element [23]. The ORF YMR279C was proposed to encode a back-up boron pump [24] while ORF YOR378W is not required for boron tolerance [23,24] but determines yeast resistance to cycloheximide and streptomycin and sensitivity to rapamycin [25,26]. Vba1p, Vba2p and Vba3p were found to be involved in vacuolar uptake of basic amino acids, mediating the transport of histidine and lysine into the vacuole [27] and Vba2p was also found to catalyze the vacuolar transport of arginine [27].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The drug:H+ antiporters of family 2 (DHA2), siderophore transporters (ARN) and glutathione:H+ antiporters (GEX) have a common evolutionary origin in hemiascomycete yeasts

Dias

Sá‐Correia

2013

BMC Genomics

View full text Add to dashboard Cite

BackgroundThe Saccharomyces cerevisiae 14-spanner Drug:H+ Antiporter family 2 (DHA2) are transporters of the Major Facilitator Superfamily (MFS) involved in multidrug resistance (MDR). Although poorly characterized, DHA2 family members were found to participate in the export of structurally and functionally unrelated compounds or in the uptake of amino acids into the vacuole or the cell. In S. cerevisiae, the four ARN/SIT family members encode siderophore transporters and the two GEX family members encode glutathione extrusion pumps. The evolutionary history of DHA2, ARN and GEX genes, encoding 14-spanner MFS transporters, is reconstructed in this study.ResultsThe translated ORFs of 31 strains from 25 hemiascomycetous species, including 10 pathogenic Candida species, were compared using a local sequence similarity algorithm. The constraining and traversing of a network representing the pairwise similarity data gathered 355 full size proteins and retrieved ARN and GEX family members together with DHA2 transporters, suggesting the existence of a close phylogenetic relationship among these 14-spanner major facilitators. Gene neighbourhood analysis was combined with tree construction methodologies to reconstruct their evolutionary history and 7 DHA2 gene lineages, 5 ARN gene lineages, and 1 GEX gene lineage, were identified. The S. cerevisiae DHA2 proteins Sge1, Azr1, Vba3 and Vba5 co-clustered in a large phylogenetic branch, the ATR1 and YMR279C genes were proposed to be paralogs formed during the Whole Genome Duplication (WGD) whereas the closely related ORF YOR378W resides in its own lineage. Homologs of S. cerevisiae DHA2 vacuolar proteins Vba1, Vba2 and Vba4 occur widespread in the Hemiascomycetes. Arn1/Arn2 homologs were only found in species belonging to the Saccharomyces complex and are more abundant in the pre-WGD species. Arn4 homologs were only found in sub-telomeric regions of species belonging to the Sacharomyces sensu strictu group (SSSG). Arn3 type siderophore transporters are abundant in the Hemiascomycetes and form an ancient gene lineage extending to the filamentous fungi.ConclusionsThe evolutionary history of DHA2, ARN and GEX genes was reconstructed and a common evolutionary root shared by the encoded proteins is hypothesized. A new protein family, denominated DAG, is proposed to span these three phylogenetic subfamilies of 14-spanner MFS transporters.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The drug:H+ antiporters of family 2 (DHA2), siderophore transporters (ARN) and glutathione:H+ antiporters (GEX) have a common evolutionary origin in hemiascomycete yeasts

Dias

Sá‐Correia

2013

BMC Genomics

View full text Add to dashboard Cite

show abstract

“…A phosphate transporter, Pho88p, has been identified as a partner of Bor1p in a split-ubiquitin-based yeast two-hybrid screen (646), suggesting that Bor1p may be part of a larger integral membrane protein complex. In light of the observations that Bor1p is 1) not downregulated under boron-limiting conditions (442), 2) not strongly upregulated by high borate levels (442), 3) not the sole candidate borate transporter in this organism (116,476,689), and 4) does not compensate for boron efflux defects in an ATR1-deletion strain (476), 11 it has been suggested that Bor1p may have another as yet uncharacterized function (442).…”

Section: B Fungimentioning

confidence: 97%

The Divergence, Actions, Roles, and Relatives of Sodium-Coupled Bicarbonate Transporters

Parker

Boron

2013

Physiological Reviews

248

262

View full text Add to dashboard Cite

The mammalian Slc4 (Solute carrier 4) family of transporters is a functionally diverse group of 10 multi-spanning membrane proteins that includes three Cl-HCO3 exchangers (AE1-3), five Na(+)-coupled HCO3(-) transporters (NCBTs), and two other unusual members (AE4, BTR1). In this review, we mainly focus on the five mammalian NCBTs-NBCe1, NBCe2, NBCn1, NDCBE, and NBCn2. Each plays a specialized role in maintaining intracellular pH and, by contributing to the movement of HCO3(-) across epithelia, in maintaining whole-body pH and otherwise contributing to epithelial transport. Disruptions involving NCBT genes are linked to blindness, deafness, proximal renal tubular acidosis, mental retardation, and epilepsy. We also review AE1-3, AE4, and BTR1, addressing their relevance to the study of NCBTs. This review draws together recent advances in our understanding of the phylogenetic origins and physiological relevance of NCBTs and their progenitors. Underlying these advances is progress in such diverse disciplines as physiology, molecular biology, genetics, immunocytochemistry, proteomics, and structural biology. This review highlights the key similarities and differences between individual NCBTs and the genes that encode them and also clarifies the sometimes confusing NCBT nomenclature.

show abstract

“…Conversely, several DHA2 proteins have been assigned substrates of biological significance, but have not been described as resistance determinants. Following the identification of ATR1 in a genetic screen for boron tolerance (Kaya et al, 2009), YMR279c , a paralog of ATR1 , was also suggested to encode a boron exporter and be involved in boron tolerance, but not YOR378w , another paralog of ATR1 (Bozdag et al, 2011). Interestingly, ATR1 was also suggested to be involved in the response to DNA replication stress, with its protein abundance being increased following MMS treatment (Tkach et al, 2012).…”

Section: Multidrug/multixenobiotic Transporters Of the Major Facilitamentioning

confidence: 99%

MFS transporters required for multidrug/multixenobiotic (MD/MX) resistance in the model yeast: understanding their physiological function through post-genomic approaches

Santos¹,

Teixeira²,

Dias³

et al. 2014

Front. Physiol.

View full text Add to dashboard Cite

Multidrug/Multixenobiotic resistance (MDR/MXR) is a widespread phenomenon with clinical, agricultural and biotechnological implications, where MDR/MXR transporters that are presumably able to catalyze the efflux of multiple cytotoxic compounds play a key role in the acquisition of resistance. However, although these proteins have been traditionally considered drug exporters, the physiological function of MDR/MXR transporters and the exact mechanism of their involvement in resistance to cytotoxic compounds are still open to debate. In fact, the wide range of structurally and functionally unrelated substrates that these transporters are presumably able to export has puzzled researchers for years. The discussion has now shifted toward the possibility of at least some MDR/MXR transporters exerting their effect as the result of a natural physiological role in the cell, rather than through the direct export of cytotoxic compounds, while the hypothesis that MDR/MXR transporters may have evolved in nature for other purposes than conferring chemoprotection has been gaining momentum in recent years. This review focuses on the drug transporters of the Major Facilitator Superfamily (MFS; drug:H+ antiporters) in the model yeast Saccharomyces cerevisiae. New insights into the natural roles of these transporters are described and discussed, focusing on the knowledge obtained or suggested by post-genomic research. The new information reviewed here provides clues into the unexpectedly complex roles of these transporters, including a proposed indirect regulation of the stress response machinery and control of membrane potential and/or internal pH, with a special emphasis on a genome-wide view of the regulation and evolution of MDR/MXR-MFS transporters.

show abstract

Roles of ATR1 paralogs YMR279c and YOR378w in boron stress tolerance

Cited by 13 publications

References 21 publications

The drug:H+ antiporters of family 2 (DHA2), siderophore transporters (ARN) and glutathione:H+ antiporters (GEX) have a common evolutionary origin in hemiascomycete yeasts

The drug:H+ antiporters of family 2 (DHA2), siderophore transporters (ARN) and glutathione:H+ antiporters (GEX) have a common evolutionary origin in hemiascomycete yeasts

The Divergence, Actions, Roles, and Relatives of Sodium-Coupled Bicarbonate Transporters

MFS transporters required for multidrug/multixenobiotic (MD/MX) resistance in the model yeast: understanding their physiological function through post-genomic approaches

Contact Info

Product

Resources

About