Roles of cell surface components of Escherichia coli K-12 in bacteriophage T4 infection: interaction of tail core with phospholipids

Furukawa, Hidehiko; Mizushima, Shôji

doi:10.1128/jb.150.2.916-924.1982

Cited by 35 publications

(15 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In fact, treatment of the virion with urea causes sheath contraction, but the phage retains the entire DNA. It has been shown that the phage with a contracted sheath due to urea treatment can infect host spheroplasts (Furukawa and Mizushima 1982;Furukawa et al 1979Furukawa et al , 1983.…”

Section: Formation Of the Contractile Tail Sheathmentioning

confidence: 99%

Molecular assembly and structure of the bacteriophage T4 tail

et al. 2016

View full text Add to dashboard Cite

The tail of bacteriophage T4 undergoes large structural changes upon infection while delivering the phage genome into the host cell. The baseplate is located at the distal end of the contractile tail and plays a central role in transmitting the signal to the tail sheath that the tailfibers have been adsorbed by a host bacterium. This then triggers the sheath contraction. In order to understand the mechanism of assembly and conformational changes of the baseplate upon infection, we have determined the structure of an in vitro assembled baseplate through the three-dimensional reconstruction of cryo-electron microscopy images to a resolution of 3.8 Å from electron micrographs. The atomic structure was fitted to the baseplate structure before and after sheath contraction in order to elucidate the conformational changes that occur after bacteriophage T4 has attached itself to a cell surface. The structure was also used to investigate the protease digestion of the assembly intermediates and the mutation sites of the tail genes, resulting in a number of phenotypes.

show abstract

Section: Formation Of the Contractile Tail Sheathmentioning

confidence: 99%

Molecular assembly and structure of the bacteriophage T4 tail

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Lipid components of membranes seem to play a significant role in the viral infection process of eucaryotic and procaryotic cells (18,23), and the activity of bacteriophage receptor proteins is strongly enhanced in the presence of lipids and the lipid portion of lipopolysaccharides (15,17,36). Furthermore, phosphatidylglycerol (PG) and cardiolipin (CL) in reconstituted membrane vesicles have been implicated to trigger DNA release from the T4 capsid (13).…”

mentioning

confidence: 99%

Phosphoglycerides and phospholipase C in membrane fractions of Escherichia coli B

Bayer¹,

Bayer²

1985

J Bacteriol

View full text Add to dashboard Cite

The phospholipid composition and the phospholipase C activity of envelope fractions of Escherichia coli B were determined with special consideration of fractions containing sites at which an attachment of inner and outer membranes had been observed in the electron microscope (Int.M). Phosphoglycerides labeled with [14C]palmitic acid and [3H]serine were extracted from membrane fractions and identified by two-dimensional thin-layer chromatography. The amount of phosphatidylethanolamine was highest in the outer membrane, whereas the amounts of phosphatidylglycerol and cardiolipin were highest in the inner membrane. The Int.M fractions were observed to have concentrations of phospholipids intermediate to those of the inner and outer membranes. This result supports the assumption that a concentration gradient of inner membrane-outer membrane lipids might exist at the membrane contact sites. The highest phospholipase C activity was detected in the inner membrane and Int.M fractions. The presence of phospholipase C and other lipolytic enzymes in the Int.M fractions suggests a possible involvement of adhesion sites in lipid metabolism, adding a further set of activities to the function of these domains.

show abstract

“…The lipoprotein-bearing peptidoglycan layer that underlies the outer membrane allows the OmpC protein and lipopolysaccharide to assemble widely on its surface so that the receptor complex can interact with the individual distal ends of the long tail fibers of a single phage particle (9). It also serves as a rigid support for the outer membrane to help the penetration of the phage core through the flexible outer membrane (8). The core tip that has penetrated the cell surface interacts with a phospholipid bilayer, representative of the cytoplasmic membrane, and the interaction induces the ejection of phage DNA through the core (8).…”

mentioning

confidence: 99%

“…It also serves as a rigid support for the outer membrane to help the penetration of the phage core through the flexible outer membrane (8). The core tip that has penetrated the cell surface interacts with a phospholipid bilayer, representative of the cytoplasmic membrane, and the interaction induces the ejection of phage DNA through the core (8). However, the DNA molecule thus ejected is not incorporated into the liposomes.…”

mentioning

confidence: 99%

DNA injection during bacteriophage T4 infection of Escherichia coli

Furukawa¹,

Kuroiwa²,

Mizushima³

1983

J Bacteriol

Self Cite

View full text Add to dashboard Cite

The process of phage T4 DNA injection into the host cell was studied under a fluorescent microscope, using 4',6-diamidino-2-phenylindole as a DNA-specific fluorochrome. The phage DNA injection was observed when spheroplasts were infected with the artificially contracted phage particles having a protruding core. The DNA injection was mediated by the interaction of the core tip with the cytoplasmic membrane of the spheroplast. A membrane potential was not required for the process of DNA injection. On the other hand, DNA injection upon infection by intact noncontracted phage of the intact host cell was inhibited by an energy poison. Based on these observations, together with results from previous work, a model for the T4 infection process is presented, and the role of the membrane potential in the infection process is discussed.

show abstract

Roles of cell surface components of Escherichia coli K-12 in bacteriophage T4 infection: interaction of tail core with phospholipids

Cited by 35 publications

References 28 publications

Molecular assembly and structure of the bacteriophage T4 tail

Molecular assembly and structure of the bacteriophage T4 tail

Phosphoglycerides and phospholipase C in membrane fractions of Escherichia coli B

DNA injection during bacteriophage T4 infection of Escherichia coli

Contact Info

Product

Resources

About