Roles of DNA repair and membrane integrity in heat resistance of Deinococcus radiodurans

Bauermeister, Anja; Hahn, Claudia; Rettberg, Petra; Reitz, Günther; Moeller, Ralf

doi:10.1007/s00203-012-0834-x

Cited by 10 publications

(6 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…D. radiodurans irradiated with 4 kGy loses up to 30% wet weight resulting from the loss of polysaccharides into the growth medium, which suggests permeability alterations in the cell envelope ( Mitchel, 1976 ). For retaining membrane integrity, D. radiodurans cells were much more resistant to high temperatures when exposed in the dried state as opposed to cells in suspension ( Bauermeister et al , 2012 ). The R1Δ dr1790 mutant showed a high proportion of damaged membranes (20% red cells observed in the mutant field) compared with wild type and complemented mutant strains.…”

Section: Discussionmentioning

confidence: 99%

Characteristics of dr1790 disruptant and its functional analysis in Deinococcus radiodurans

Cheng¹,

Hu²,

Xu³

et al. 2015

Braz. J. Microbiol.

View full text Add to dashboard Cite

Deinococcus radiodurans (DR) is an extremophile that is well known for its resistance to radiation, oxidants and desiccation. The gene dr1790 of D. radiodurans was predicted to encode a yellow-related protein. The primary objective of the present study was to characterize the biological function of the DR1790 protein, which is a member of the ancient yellow/major royal jelly (MRJ) protein family, in prokaryotes. Fluorescence labeling demonstrated that the yellow-related protein encoded by dr1790 is a membrane protein. The deletion of the dr1790 gene decreased the cell growth rate and sensitivity to hydrogen peroxide and radiation and increased the membrane permeability of D. radiodurans. Transcript profiling by microarray and RT-PCR analyses of the dr1790 deletion mutant suggested that some genes that are involved in protein secretion and transport were strongly suppressed, while other genes that are involved in protein quality control, such as chaperones and proteases, were induced. In addition, the expression of genes with predicted functions that are involved in antioxidant systems, electron transport, and energy metabolism was significantly altered through the disruption of dr1790. Moreover, the results of proteomic analyses using 2-DE and MS also demonstrated that DR1790 contributed to D. radiodurans survival. Taken together, these results indicate that the DR1790 protein from the ancient yellow protein family plays a pleiotropic role in the survival of prokaryotic cells and contributes to the extraordinary resistance of D. radiodurans against oxidative and radiation stresses.

show abstract

Section: Discussionmentioning

confidence: 99%

Characteristics of dr1790 disruptant and its functional analysis in Deinococcus radiodurans

Cheng¹,

Hu²,

Xu³

et al. 2015

Braz. J. Microbiol.

View full text Add to dashboard Cite

show abstract

“…Previous desiccation, on the other hand, can also be beneficial to vacuum tolerance as demonstrated in storage experiments of the closely related D. radiodurans compared with storage at ambient air (Bauermeister et al, 2012). Endurance of vacuum exposure has also been demonstrated for D. radiodurans, D. aerius, and D. aetherius for 1 year with a 10-to 100-fold reduction in culturability (Kawaguchi et al, 2013).…”

Section: Enzymatic Activity Of Biofilms and Planktonic Cells Of D Gementioning

confidence: 92%

Tolerances ofDeinococcus geothermalisBiofilms and Planktonic Cells Exposed to Space and Simulated Martian Conditions in Low Earth Orbit for Almost Two Years

et al. 2019

Self Cite

View full text Add to dashboard Cite

Fossilized biofilms represent one of the oldest known confirmations of life on the Earth. The success of microbes in biofilms results from properties that are inherent in the biofilm, including enhanced interaction, protection, and biodiversity. Given the diversity of microbes that live in biofilms in harsh environments on the Earth, it is logical to hypothesize that, if microbes inhabit other bodies in the Universe, there are also biofilms on those bodies. The Biofilm Organisms Surfing Space experiment was conducted as part of the EXPOSE-R2 mission on the International Space Station. The experiment was an international collaboration designed to perform a comparative study regarding the survival of biofilms versus planktonic cells of various microorganisms, exposed to space and Mars-like conditions. The objective was to determine whether there are lifestyledependent differences to cope with the unique mixture of stress factors, including desiccation, temperature oscillations, vacuum, or a Mars-like gas atmosphere and pressure in combination with extraterrestrial or Marslike ultraviolet (UV) radiation residing during the long-term space mission. In this study, the outcome of the flight and mission ground reference analysis of Deinococcus geothermalis is presented. Cultural tests demonstrated that D. geothermalis remained viable in the desiccated state, being able to survive space and Mars-like conditions and tolerating high extraterrestrial UV radiation for more than 2 years. Culturability decreased, but was better preserved, in the biofilm consortium than in planktonic cells. These results are correlated to differences in genomic integrity after exposure, as visualized by random amplified polymorphic DNA-polymerase chain reaction. Interestingly, cultivation-independent viability markers such as membrane integrity, ATP content, and intracellular esterase activity remained nearly unaffected, indicating that subpopulations of the cells had survived in a viable but nonculturable state. These findings support the hypothesis of long-term survival of microorganisms under the harsh environmental conditions in space and on Mars to a higher degree if exposed as biofilm.

show abstract

“…Knockout of the dsr11 affects the expression of genes involved in cell membranes. Previous reports have shown that heat stress can lead to cell membrane disruption in D. radiodurans [37]. We hypothesized that the loss of dsr11 may lead to changes in cell membrane function, thus affecting heat stress tolerance.…”

Section: Rna-seq Analysis Sheds New Light On the Mechanisms Of Dsr11-mentioning

confidence: 94%

A Novel Noncoding RNA dsr11 Involved in Heat Stress Tolerance in Deinococcus radiodurans

Dong

Chen

et al. 2019

Biomolecules

View full text Add to dashboard Cite

Deinococcus radiodurans is an extremely resistant bacteria that has evolved masterful strategies to enable survival under various environmental stress conditions. Heat stress is a major environmental stress factor that can cause denaturation of proteins, membrane disruption, and oxidative stress. Previous studies have examined the mechanisms of the heat stress response by analyzing changes in protein levels; however, little is known about the role of small noncoding RNAs (ncRNAs), which are known to play important regulatory functions in bacteria during various environmental stress response. The ncRNA dsr11 of D. radiodurans was previously identified by RNA-seq and Northern blot. In this study, we showed that the transcription level of dsr11 was up-regulated 4.2-fold under heat stress by qRT-PCR analysis. Heat tolerance assay showed that deleting dsr11 significantly inhibited the viability under high temperature conditions. To assess the influence of dsr11 on the D. radiodurans transcriptome, 157 genes were found differentially expressed in the knock-out mutant by RNA-seq experiment. Combining RNA-seq and in silico analysis, we found that trmE (tRNA modification GTPase) and dr_0651 (arginase) were likely to be the direct targets of dsr11. Further microscale thermophoresis results demonstrated that dsr11 can directly bind to the mRNA of trmE and dr_0651. Our results indicated that dsr11 can enhance the tolerance to heat stress of D. radiodurans by binding to trmE and dr_0651 mRNA. Overall, these results extend our understanding of ncRNA regulation and provide new insights into the heat stress response in D. radiodurans.

show abstract

Roles of DNA repair and membrane integrity in heat resistance of Deinococcus radiodurans

Cited by 10 publications

References 43 publications

Characteristics of dr1790 disruptant and its functional analysis in Deinococcus radiodurans

Characteristics of dr1790 disruptant and its functional analysis in Deinococcus radiodurans

Tolerances ofDeinococcus geothermalisBiofilms and Planktonic Cells Exposed to Space and Simulated Martian Conditions in Low Earth Orbit for Almost Two Years

A Novel Noncoding RNA dsr11 Involved in Heat Stress Tolerance in Deinococcus radiodurans

Contact Info

Product

Resources

About