Roles of entropic and solvent damping forces in the dynamics of polymer tethered nanoparticles and implications for single molecule sensing

Ma, Guangzhong; Wan, Zijian; Zhu, Hao; Tao, Nongjian

doi:10.1039/c9sc05434k

Cited by 9 publications

(7 citation statements)

References 36 publications

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“…At around the same time, Ma et al ( 2020 ) tethered a nanoparticle with a single DNA molecule to a gold surface for studying the entropic and damping forces. A recent study conducted by Kotsifaki et al takes advantage of Fano resonance-assisted plasmonic optical tweezers (FAPOT) for single nanoparticle trapping in an array of asymmetrical split-ring (ASR) metamaterials on a gold film.…”

Section: Applications In Biomedical Engineeringmentioning

confidence: 99%

Applications of Optically Controlled Gold Nanostructures in Biomedical Engineering

Phummirat

Mann

Preece

2021

Front. Bioeng. Biotechnol.

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Since their inception, optical tweezers have proven to be a useful tool for improving human understanding of the microscopic world with wide-ranging applications across science. In recent years, they have found many particularly appealing applications in the field of biomedical engineering which harnesses the knowledge and skills in engineering to tackle problems in biology and medicine. Notably, metallic nanostructures like gold nanoparticles have proven to be an excellent tool for OT-based micromanipulation due to their large polarizability and relatively low cytotoxicity. In this article, we review the progress made in the application of optically trapped gold nanomaterials to problems in bioengineering. After an introduction to the basic methods of optical trapping, we give an overview of potential applications to bioengineering specifically: nano/biomaterials, microfluidics, drug delivery, biosensing, biophotonics and imaging, and mechanobiology/single-molecule biophysics. We highlight the recent research progress, discuss challenges, and provide possible future directions in this field.

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Section: Applications In Biomedical Engineeringmentioning

confidence: 99%

Applications of Optically Controlled Gold Nanostructures in Biomedical Engineering

Phummirat

Mann

Preece

2021

Front. Bioeng. Biotechnol.

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“…However, the detection signal diminishes with decreasing molecular mass, which makes it challenging to probe small-molecule binding. Recently, charge-sensitive detection technology for small-molecule binding has emerged, which uses oscillators driven by an external electrical field. − This plasmonic imaging-based technique has demonstrated its potential applications in measuring phosphorylation and ligand binding to membrane proteins, − but it suffers from several limitations. To excite surface plasmons, a gold surface is usually used, which generates strong background variations in an electrical field due to the charging effect of the gold film, , washing out the tiny signals from the oscillators.…”

Section: Introductionmentioning

confidence: 99%

Label-Free Probing of Molecule Binding Kinetics Using Single-Particle Interferometric Imaging

et al. 2021

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Probing molecular interactions is critical for screening drugs, detecting pollutants, and understanding biological processes at the molecular level, but these interactions are difficult to detect, especially for small molecules. A label-free optical imaging technology that can detect molecule binding kinetics is presented, in which free-moving particles are driven into oscillations with an alternating electrical field and the interferometric scattering patterns of the particles are imaged via an optical imaging method. By tracking the charge-sensitive variations in the oscillation amplitude with sub-nanometer precision, the small molecules and metal ions binding to the surface as well as protein−protein binding kinetics were measured. The capability of the label-free measurement of molecular interactions can provide a promising platform for screening small-molecule drugs, probing conformational changes in proteins, and detecting environmental pollutants.

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“…In contrast to the steep 1/ r 6 distance dependence of FRET efficiency, coupled plasmonic nanoparticles display 1/ r 3 signal dependence that enables sensing over a longer range in distance ( r plasmon ∼ 0–30 nm vs r FRET < 10 nm) . However, when using tethered nanoparticles as plasmonic rulers, care must be taken to ensure the system exists in the regime where its dynamics are dominated by the biomolecule and not by solvent damping of the nanoparticles . Detection of the distance-dependent spectral shifts of coupled plasmonic nanoparticles can achieve sub-nm resolution on their separation, but the need for spectral dispersion can reduce temporal resolution.…”

Section: Introductionmentioning

confidence: 99%

“…47 However, when using tethered nanoparticles as plasmonic rulers, care must be taken to ensure the system exists in the regime where its dynamics are dominated by the biomolecule and not by solvent damping of the nano- particles. 48 Detection of the distance-dependent spectral shifts of coupled plasmonic nanoparticles can achieve sub-nm resolution on their separation, 49 but the need for spectral dispersion can reduce temporal resolution. When the intensity of scattered light is used as a readout, the substantial photon scattering from individual plasmonic particles can sustain fast (∼μs) temporal resolution.…”

Section: ■ Introductionmentioning

confidence: 99%

Measuring the Multiscale Dynamics, Structure, and Function of Biomolecules at Interfaces

Noriega

2021

J. Phys. Chem. B

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The individual and collective structure and properties of biomolecules can change dramatically when they are localized at an interface. However, the small spatial extent of interfacial regions poses challenges to the detailed characterization of multiscale processes that dictate the structure and function of large biological units such as peptides, proteins, or nucleic acids. This Perspective surveys a broad set of tools that provide new opportunities to probe complex, dynamic interfaces across the vast range of temporal regimes that connect molecular-scale events to macroscopic observables. An emphasis is placed on the integration over multiple time scales, the use of complementary techniques, and the incorporation of external stimuli to control interfacial properties with spatial, temporal, and chemical specificity.

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Roles of entropic and solvent damping forces in the dynamics of polymer tethered nanoparticles and implications for single molecule sensing

Abstract: Tethering a particle to a surface with a single molecule allows detection of the molecule and analysis of molecular conformations and interactions.

Cited by 9 publications

References 36 publications

Applications of Optically Controlled Gold Nanostructures in Biomedical Engineering

Applications of Optically Controlled Gold Nanostructures in Biomedical Engineering

Label-Free Probing of Molecule Binding Kinetics Using Single-Particle Interferometric Imaging

Measuring the Multiscale Dynamics, Structure, and Function of Biomolecules at Interfaces

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