Roles of Formin Nodes and Myosin Motor Activity in Mid1p-dependent Contractile-Ring Assembly during Fission Yeast Cytokinesis

Coffman, Valerie C.; Nile, Aaron H.; Lee, I-Ju; Liu, Huayang; Wu, Jian-Qiu

doi:10.1091/mbc.e09-05-0428

Cited by 103 publications

(173 citation statements)

References 67 publications

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“…Mid1 is the critical protein necessary for the recruitment and early scaffolding of these components [Wu et al, 2003;Celton-Morizur et al, 2004;Motegi et al, 2004;Wu et al, 2006;Coffman et al, 2009;Almonacid et al, 2011;Laporte et al, 2011;Padmanabhan et al, 2011].…”

Section: Maturation Of Medial Cortical Nodes Into Cytokinetic Corticamentioning

confidence: 99%

“…Other major proteins recruited to the cytokinetic nodes besides the IQGAP protein Rng2 and myosin II are the F-BAR protein Cdc15 and the formin Cdc12 [Wu et al, 2003;Wu et al, 2006;Coffman et al, 2009;Laporte et al, 2011]. Careful analysis of the localization of contractile ring components in a variety of mutants has led to a model in which Mid1 organizes two independent modules.…”

Section: Maturation Of Medial Cortical Nodes Into Cytokinetic Corticamentioning

confidence: 99%

Section: Mechanisms Of Contractile Ring Assemblymentioning

confidence: 99%

“…This event is triggered by the Plo1-dependent phosphorylation of Mid1 N-terminal fragment which can bind Rng2 directly in vitro [Almonacid et al, 2011]. Noteworthy, the phosphorylation by Plo1 of the same set of amino acids within Mid1 1-100 fragment thus controls at the same time Mid1 activation and Mid1 export from the nucleus, directly linking these two events.Other major proteins recruited to the cytokinetic nodes besides the IQGAP protein Rng2 and myosin II are the F-BAR protein Cdc15 and the formin Cdc12 [Wu et al, 2003;Wu et al, 2006;Coffman et al, 2009;Laporte et al, 2011]. Careful analysis of the localization of contractile ring components in a variety of mutants has led to a model in which Mid1 organizes two independent modules.…”

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confidence: 99%

“…The alternate Cdc12 Spot-Leading Cable model proposes that a major Cdc12 spot moves toward the cell middle and nucleates an actin leading cable beneath the plasma membrane and perpendicular to the long axis of the cell, which eventually gives rise to the contractile ring [Chang, 1999;Kamasaki et al, 2007]. The facts that Cdc12 spot is not essential for Cdc12 function [Yonetani et al, 2008] and does not seem to nucleate actin filaments and that Myo2 activity is necessary for ring compaction [Coffman et al, 2009] rather favor the SCPR over the Cdc12 Spot-Leading Cable model at this stage.Although Mid1 is a major spatial cue for contractile ring assembly, fission yeast cells can assemble a functional contractile ring in the absence of Mid1, though inefficiently and at random locations Sohrmann et al, 1996]. In this case, the contractile ring is formed from actomyosin filaments assembled randomly on the cortex, which eventually converge to assemble a contractile ring [Huang et al, 2008].…”

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Mid1/anillin and the spatial regulation of cytokinesis in fission yeast

Rincón

Paoletti

2012

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Cell division is a critical and irreversible step in the cell cycle. The strategies that cells follow to regulate the position of the division plane must take into account the global geometry of the cell as well as position of the genetic material to ensure its accurate segregation into daughter cells of a given cell shape and size. Along the years, research on Schizosaccharomyces pombe, a wellrecognized model organism for cell division studies has allowed a detailed molecular understanding of the spatial mechanisms regulating cytokinesis. Division plane position in this unicellular rod-shaped organism, which divides by the assembly and constriction of a medially placed actomyosin ring, largely depends on the anillinlike protein Mid1. Therefore, the major pathways controlling the position of the division plane converge on Mid1. In this review, we make an overview of the studies that have deciphered how Mid1 localization and scaffolding activities are controlled over the cell cycle to ensure the symmetrical division of fission yeast cells. These studies have revealed new mechanisms generating spatial information based on nuclear shuttling of the division plane factor Mid1 and on the establishment of cortical inhibitory gradients of the cell polarity kinase Pom1. V C 2012 Wiley Periodicals, Inc Key Words: cytokinesis, fission yeast, contractile ring, Mid1, cell division Introduction C ytokinesis is the final step of cell division that physically separates the daughter cells at the end of the cell cycle. It is a universal process, essential for cell proliferation, for the survival of unicellular organisms or for the development of multicellular organisms, whose major features have been well conserved along evolution. As an irreversible event, cytokinesis is under tight spatial and temporal regulation. Defective cytokinesis can indeed alter cell geometry or size, perturb cellular organization within tissues or prevent the accurate transmission of the genetic material, producing aneuploid cells, which can affect the survival of unicellular species or favor cancer and tumor progression in animal species.Cytokinesis is first strictly coordinated with mitotic progression in order to successfully fulfill chromosome segregation. Checkpoint mechanisms monitoring the function of the mitotic apparatus can halt cells in metaphase, which prevents cytokinesis until actual chromosome segregation in anaphase.Second, spatial regulatory pathways define the position of the division plane according to the position of the nucleus at mitotic entry, or of the mitotic apparatus, perpendicular to the axis of chromosome segregation. This ensures the efficient segregation of the genetic material and allows to control the geometry of the daughter cells in response to morphogenetic programs. Cell division can indeed be symmetric or asymmetric to allow the generation of daughter cells of different sizes. Orientation of the division plane is also modulated to properly position the daughter cells inside embryos or tissues and to permit the asymm...

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Section: Maturation Of Medial Cortical Nodes Into Cytokinetic Corticamentioning

confidence: 99%

Section: Maturation Of Medial Cortical Nodes Into Cytokinetic Corticamentioning

confidence: 99%

Section: Mechanisms Of Contractile Ring Assemblymentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Mid1/anillin and the spatial regulation of cytokinesis in fission yeast

Rincón

Paoletti

2012

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Dividing the spoils of growth and the cell cycle: The fission yeast as a model for the study of cytokinesis

et al. 2011

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Cytokinesis is the final stage of the cell cycle, and ensures completion of both genome segregation and organelle distribution to the daughter cells. Cytokinesis requires the cell to solve a spatial problem (to divide in the correct place, orthogonally to the plane of chromosome segregation) and a temporal problem (to coordinate cytokinesis with mitosis). Defects in the spatiotemporal control of cytokinesis may cause cell death, or increase the risk of tumor formation [Fujiwara et al., 2005 (Fujiwara T, Bandi M, Nitta M, Ivanova EV, Bronson RT, Pellman D. 2005. Cytokinesis failure generating tetraploids promotes tumorigenesis in p53-null cells. Nature 437:1043–1047); reviewed by Ganem et al., 2007 (Ganem NJ, Storchova Z, Pellman D. 2007. Tetraploidy, aneuploidy and cancer. Curr Opin Genet Dev 17:157–162.)]. Asymmetric cytokinesis, which permits the generation of two daughter cells that differ in their shape, size and properties, is important both during development, and for cellular homeostasis in multicellular organisms [reviewed by Li, 2007 (Li R. 2007. Cytokinesis in development and disease: variations on a common theme. Cell Mol Life Sci 64:3044–3058)]. The principal focus of this review will be the mechanisms of cytokinesis in the mitotic cycle of the yeast Schizosaccharomyces pombe. This simple model has contributed significantly to our understanding of how the cell cycle is regulated, and serves as an excellent model for studying aspects of cytokinesis. Here we will discuss the state of our knowledge of how the contractile ring is assembled and disassembled, how it contracts, and what we know of the regulatory mechanisms that control these events and assure their coordination with chromosome segregation. © 2011 Wiley-Liss, Inc.

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Contractile‐ring assembly in fission yeast cytokinesis: Recent advances and new perspectives

Lee

Coffman

2012

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The fission yeast Schizosaccharomyces pombe is an excellent model organism to study cytokinesis. Here, we review recent advances on contractile-ring assembly in fission yeast. First, we summarize the assembly of cytokinesis nodes, the precursors of a normal contractile ring. IQGAP Rng2 and myosin essential light chain Cdc4 are recruited by the anillin-like protein Mid1, followed by the addition of other cytokinesis node proteins. Mid1 localization on the plasma membrane is stabilized by interphase node proteins. Second, we discuss proteins and processes that contribute to the search, capture, pull, and release mechanism of contractile-ring assembly. Actin filaments nucleated by formin Cdc12, the motor activity of myosin-II, the stiffness of the actin network, and severing of actin filaments by cofilin all play essential roles in contractile-ring assembly. Finally, we discuss the Mid1-independent pathway for ring assembly, and the possible mechanisms underlying the ring maturation and constriction. Collectively, we provide an overview of the current understanding of contractile-ring assembly and uncover future directions in studying cytokinesis in fission yeast.

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Roles of Formin Nodes and Myosin Motor Activity in Mid1p-dependent Contractile-Ring Assembly during Fission Yeast Cytokinesis

Cited by 103 publications

References 67 publications

Mid1/anillin and the spatial regulation of cytokinesis in fission yeast

Mid1/anillin and the spatial regulation of cytokinesis in fission yeast

Dividing the spoils of growth and the cell cycle: The fission yeast as a model for the study of cytokinesis

Contractile‐ring assembly in fission yeast cytokinesis: Recent advances and new perspectives

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