Roles of insulin-like growth factor-I (IGF-I) and IGF-I binding protein-2 (IGFBP2) and -5 (IGFBP5) in developing chick limbs

McQueeney, Kelly; Dealy, Caroline N.

doi:10.1054/ghir.2001.0250

Cited by 41 publications

(31 citation statements)

References 85 publications

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“…The observations we report here, together with several other recent reports (McQueeney and Dealy, 2001;Allan et al, 2003), point to a previously unsuspected link between the Shh and insulin-like growth factor (IGF) pathways. Although the role of Shh signaling in prostate development has been a topic of considerable interest, the role of the IGF signaling axis in prostate development has not been well described.…”

Section: Discussionsupporting

confidence: 80%

Sonic hedgehog signaling regulates the expression of insulin‐like growth factor binding protein‐6 during fetal prostate development

Lipinski¹,

Cook²,

Barnett³

et al. 2005

Developmental Dynamics

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At the onset of ductal morphogenesis in the developing prostate, Shh expression condenses at evaginations of urogenital sinus epithelium and activates Gli transcription factors in the adjacent mesenchyme. Abrogation of Hedgehog signaling disrupts proper prostatic budding, ductal growth, and branching. We now show that Hedgehog signaling regulates the expression of insulin-like growth factor binding protein-6 (Igfbp-6) in the developing mouse prostate. Igfbp-6 is a secreted factor that specifically binds insulin-like growth factor-II (IGF-II), prevents its binding to the IGF-I receptor, and is thought to regulate the activity of IGF-II in growth and differentiation. Igfbp-6 is expressed in both the developing and adult prostate. In the urogenital sinus, Igfbp-6 mRNA colocalized with Ptc1 and Gli1 mRNA in the mesenchyme, while Igfbp-6 protein was found in both the mesenchymal and epithelial layers. Exogenous Shh peptide induced expression of Igfbp-6 in the developing prostate while the chemical inhibitor of Hedgehog signaling, cyclopamine, reduced its expression. These studies show that Igfbp-6 is an actual target of Shh signaling in the urogenital sinus and provide the first evidence for a linkage between the Hedgehog and IGF signaling pathways in prostate development. Developmental Dynamics 233:829 -836, 2005.

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Section: Discussionsupporting

confidence: 80%

Sonic hedgehog signaling regulates the expression of insulin‐like growth factor binding protein‐6 during fetal prostate development

Lipinski¹,

Cook²,

Barnett³

et al. 2005

Developmental Dynamics

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“…While Lbx1h is expressed in developing chick EOM (5, 53), it is restricted to only one of the six muscles (i.e., a minority source of myoblasts for the mEOM line) and has not been reported in mammalian EOM. Likewise, Ebf1, Hoxc10, Igfbp2, Kremen, Popdc3, Robo1, Six1, and Wnt5a are all established limb development markers (2,6,15,47,50,51,54,92) that either are expressed at higher levels or differentially regulated only in mLM. Collectively, these expression patterns, and the other patterns in signaling pathways (see below), help validate the mLM line as a model for limb muscle development.…”

Section: Discussionmentioning

confidence: 99%

Distinctive morphological and gene/protein expression signatures during myogenesis in novel cell lines from extraocular and hindlimb muscle

Porter

Israel

Gong

et al. 2006

Physiological Genomics

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Skeletal muscles are not created equal. The underutilized concept of muscle allotypes defines distinct muscle groups that differ in their intrinsic capacity to express novel traits when exposed to a facilitating extrinsic environment. Allotype-specific traits may have significance as determinants of the preferential involvement or sparing of muscle groups that is observed in a variety of neuromuscular diseases. Little is known, however, of the developmental mechanisms underlying the distinctive skeletal muscle allotypes. The lack of appropriate in vitro models, to dissociate the cell-autonomous and non-cell-autonomous mechanisms behind allotype diversity, has been a barrier to such studies. Here, we derived novel cell lines from the extraocular and hindlimb muscle allotypes and assessed their similarities and differences during early myogenesis using morphological and gene/protein expression profiling tools. Our data establish that there are fundamental differences in the transcriptional and cellular signaling pathways used by the two myoblast lineages. Taken together, these data show that myoblast lineage plays a significant role in the divergence of the distinctive muscle groups or allotypes.

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“…This could be the reason for the enhanced chondrogenic response of hUCMSC to TGFβ3 growth factor. IGF also plays an important role in skeletal development, as indicated by various studies (McQueeney and Dealy, 2001;Steinert et al, 2009). The biological action of IGFs is transduced by the type I tyrosine kinase receptor (IGFIR) that classically activates the mitogen-activated protein kinase/extracellular signalregulated kinase-kinase 1/2 (MEK1/2), extracellular RS Nirmal et al…”

Section: Rs Nirmal Et Al In Vitro Chondrogenesis Of Hucmscmentioning

confidence: 99%

Significance of soluble growth factors in the chondrogenic response of human umbilical cord matrix stem cells in a porous three dimensional scaffold

Nirmal

Nair²

2013

eCM

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Stem cell based tissue engineering has emerged as a promising strategy for articular cartilage regeneration. Foetal derived mesenchymal stem cells (MSCs) with their ease of availability, pluripotency and high expansion potential have been demonstrated to be an attractive cell source over adult MSCs. However, there is a need for optimisation of chondrogenic signals to direct the differentiation of these multipotent MSCs to chondrogenic lineage. In this study we have demonstrated the in vitro chondrogenesis of human umbilical cord matrix MSCs in three dimensional PVA-PCL (polyvinyl alcoholpolycaprolactone) scaffolds in the presence of the individual growth factors TGFβ1, TGFβ3, IGF, BMP2 and their combination with BMP2. Gene expression, histology and immunohistology were evaluated after 28 d culture. The induced cells showed the feature of chondrocytes in their morphology and expression of typical chondrogenic extracellular matrix molecules. Moreover, the real-time PCR assay has shown the expression of gene markers of chondrogenesis, SOX9, collagen type II and aggrecan. The expression of collagen type I and collagen type X was also evaluated. This study has demonstrated the successful chondrogenic induction of human umbilical cord MSCs in 3D scaffolds. Interestingly, the growth factor combination of TGF-β3 and BMP-2 was found to be more effective for chondrogenesis as shown by the real-time PCR studies. The findings of this study suggest the importance of using growth factor combinations for successful chondrogenic differentiation of umbilical cord MSCs.

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Roles of insulin-like growth factor-I (IGF-I) and IGF-I binding protein-2 (IGFBP2) and -5 (IGFBP5) in developing chick limbs

Cited by 41 publications

References 85 publications

Sonic hedgehog signaling regulates the expression of insulin‐like growth factor binding protein‐6 during fetal prostate development

Sonic hedgehog signaling regulates the expression of insulin‐like growth factor binding protein‐6 during fetal prostate development

Distinctive morphological and gene/protein expression signatures during myogenesis in novel cell lines from extraocular and hindlimb muscle

Significance of soluble growth factors in the chondrogenic response of human umbilical cord matrix stem cells in a porous three dimensional scaffold

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