This study investigates the impact of ambient conditions on the performance of P3HT/Ru-dye/nc-TiO2 solar cells (TLSCs). It has been found that the increase of temperature and irradiation duration affect on the parameters of TLSCs. When the temperature was increased from 293 k to 393 k , the short circuit current density (Jsc ) and open circuit voltgae (Voc) decrease from 2.2 to 1.7 mA/cm2 and 0.7 V to 0.5 V respectively. That is attributed to the effect of high temperature on recombination of photo-generated charges and reduction of shunt resistance (Rsh ) in the TLSC. Moreover, we also present the effect of irradiation duration on performance of the TLSCs. The measurement reveals that Jsc decreased by 0.5 mA/cm2 while Voc decreased by ~ 0.18 V during 4800 s illumination. This decrease suggests the filling of traps or defects at the interface with photo-genertated charges. Finally, the maximum output power of TLSCs dropped by almost half within 6 days during a 20-day test because of affecting atmosphere moisture on the interface properties between dye/ nc-TiO2 and P3HT.