Roles of lipid peroxidation and cytoplasmic droplets on in vitro fertilization capacity of sperm collected from bovine epididymides stored at 4 and 34°C

Nichi, Marcílio; Goovaerts, Inge; Cortada, Carmen Neusa Martins; Barnabé, Valquíria Hyppólito; Clercq, J. B. P. De; Bols, P.E.J.

doi:10.1016/j.theriogenology.2006.08.002

Cited by 85 publications

(91 citation statements)

References 36 publications

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“…We have observed this phenomenon from the 48 h of testicle conservation, more marked at 72 h, when the R-stored samples had significantly higher percentage of abnormal sperm form (+83%) than the E-stored samples. Cold shock seems to influence in a significant way the percentage of cytoplasmatic droplets (both proximal and distal) at 72 h after death, inducing the loss of sperm droplets (Nichi et al, 2007), phenomenon confirmed in our experiment where these spermatic structures were lower in refrigerated samples.…”

Section: Discussionsupporting

confidence: 88%

Effect of testicle postmortem storage on goat frozen-thawed epididymal sperm quality as a tool to improve genebanking in local breeds

Turri

Madeddu

Gliozzi

et al. 2014

Animal

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The interest to develop assisted reproductive technologies and cryobanking for farm animal genetic resource conservation has recently increased. However, cryopreservation for ex-situ management of genetic diversity sometimes is not routinely feasible, owing to the lack of facilities (AI centres, laboratories) and expertise near the local breed farming area. In these cases, epididymal sperm obtained from slaughtered or castrated animals, associated with the possibility of managing rather long periods between animal death, sperm recovery and freezing, would increase the opportunities to create semen storages. This investigation addresses the pre-freeze/post-thaw quality of goat epididymal sperm as a function of testicle storage temperature (environment or +5°C) and time elapsed between animal's death and sperm recovery (0, 24, 48, 72 h) to establish the optimal protocols for the recovery and cryopreservation of epididymal sperm in this species. Testicles of 50 mature bucks collected at the abattoir were divided in two groups: half of the testicles (n = 50) were transported to the laboratory at environment temperature (E), whereas the remaining half (n = 50) at a refrigeration temperature (R) of +5°C. In the two groups (E) and (R), one testicle from each pair was processed after slaughter forming the time 0 groups (0E and 0R). The contralateral testicle was processed after 24, 48 or 72 h of storage, at the corresponding temperature. Sperm motility and kinetic parameters, viability and morphology were assessed in pre-freeze and post-thaw samples. Until 48 h postmortem, both E and R temperatures are able to maintain good pre-freeze epididymal sperm quality. After 48 h postmortem, R temperature is fundamental to reduce epididymal sperm quality decay in pre-freeze samples. Moreover, testicle refrigeration also has a positive impact on post-thaw samples, allowing a lower decline through time considering total motility, kinetics parameters, sperm viability and sperm abnormalities. Therefore, when sperm cryopreservation is not immediately practicable, goat testicles should be transported and stored at 5°C up to a maximum of 48 h postmortem to ensure an acceptable sperm quality.

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Section: Discussionsupporting

confidence: 88%

Effect of testicle postmortem storage on goat frozen-thawed epididymal sperm quality as a tool to improve genebanking in local breeds

Turri

Madeddu

Gliozzi

et al. 2014

Animal

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“…The extender, storage time, and the interaction between extender and storage time, however, affected spermatozoa motility and velocity throughout the storage time in both Ham's F10 and TCM-199, compared with Triladyl. The reduction in sperm motility characteristics could be due to what previous studies have reported on oxidative stress (Nichi et al, 2007;Sarıözkan et al, 2009). Reactive oxygen species (ROS) produced by damaged spermatozoa phospholipids, which are toxic to normal spermatozoa, could be mitigated by the use of antioxidants at appropriate optimal concentrations.…”

Section: Resultsmentioning

confidence: 96%

Viability of bull semen extended with commercial semen extender and two culture media stored at 24 °C

Raseona¹,

Ajao²,

Nethengwe³

et al. 2017

SA J. An. Sci.

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The aim of this study was to evaluate the viability of bull spermatozoa diluted with commercial semen extender and two culture media stored at controlled room temperature (24 °C) for 72 hours. Two Nguni bulls were used for semen collection with the aid of an electro-ejaculator. After macroscopic evaluation, semen was pooled and aliquoted randomly into Triladyl, modified Ham's F10, and TCM-199 culture media, and then stored at 24 °C. Sperm motility parameters, morphology, and viability were analysed with computer aided sperm analysis (CASA) after 0, 24, 48 and 72 hours. The study was replicated four times, and data were analysed using analysis of variance (ANOVA). Triladyl had significantly higher sperm viability rate (41.3%) and total motility rate (96.3%) for 72 hours than modified Ham's F10 (86.8%; 26.5%) and TCM-199 (76.7%; 25.0%) culture media. Ham's F10 had higher progressive motility rate (37.8%) than the other extenders TCM-199 (31.7%) and Triladyl (23.4%). There was no significant difference in viability rate between Ham's F10 (26.5%) and TCM-199 (25.0 %) after 72 hours' storage at 24 °C. Furthermore, no significant difference was observed in total sperm abnormalities, except for reacted acrosomes and absent tails, between the two Nguni bulls. Nguni semen can be preserved in Triladyl or modified Ham's F10 and TCM-199 culture media, stored at 24 °C, and stay viable for 72 hours.

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“…Analysis of the induced oxidative stress followed by the protocol described by Nichi et al (2007). (125μl, 4mM) and sodium ascorbate (125μl, 20mM) for 1.5 hours at 37°C.…”

Section: Methodsmentioning

confidence: 99%

The use of reduced glutathione (GSH) as antioxidant for cryopreserved sperm in dogs

Angrimani

Nichi

Brito

et al. 2018

Arq. Bras. Med. Vet. Zootec.

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The aim of this study was to evaluate the effect of supplementation with different concentrations of reduced glutathione GSH (0; 5; 7.5; 10mM) in the extender for cryopreservation in dogs with evaluations performed after glycerolization (chilled) and thawing (thawed). For this purpose, we used 8 dogs and two semen collections were performed in a weekly interval, totaling 16 semen samples. The sperm were analyzed by automatic sperm motility (CASA) and flow cytometry analysis of mitochondrial potential (JC1 dye) and membrane/acrosome integrity (FITC-PI dyes). We evaluated subjectively the membrane and acrosome integrity, mitochondrial activity and DNA integrity. Seminal plasma was evaluated for lipid peroxidation (TBARS concentration). Chilled and thawed samples supplemented with 7.5 and 10mM of GSH had lower percentage of sperm with high (DAB -Class I) and medium (DAB -Class II) mitochondrial activity. And 10mM of GSH had higher percentage of low mitochondrial activity (DABClass III). Moreover, thawed samples of 10mM of GSH had high DNA fragmentation rates. Probably by a reductive stress effect on mitochondria which lead to an increase in reactive oxygen species, and a mitochondrial malfunction.Keywords: reactive oxygen species, cryopreservation, antioxidant, canine RESUMOO objetivo deste estudo foi avaliar o efeito da suplementação com diferentes concentrações de glutationa reduzida 5; 7,5; 10mM)

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Roles of lipid peroxidation and cytoplasmic droplets on in vitro fertilization capacity of sperm collected from bovine epididymides stored at 4 and 34°C

Cited by 85 publications

References 36 publications

Effect of testicle postmortem storage on goat frozen-thawed epididymal sperm quality as a tool to improve genebanking in local breeds

Effect of testicle postmortem storage on goat frozen-thawed epididymal sperm quality as a tool to improve genebanking in local breeds

Viability of bull semen extended with commercial semen extender and two culture media stored at 24 °C

The use of reduced glutathione (GSH) as antioxidant for cryopreserved sperm in dogs

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