Roles of luteinizing hormone/chorionic gonadotropin receptor in anchorage‐dependent and ‐independent growth in human ovarian surface epithelial cell lines

Tashiro, Hironori; Katabuchi, Hidetaka; Begum, Monjura; Li, Xiaoguang; Nitta, Makoto; Ohtake, Hideyuki; Okamura, Hitoshi

doi:10.1111/j.1349-7006.2003.tb01384.x

Cited by 25 publications

(19 citation statements)

References 24 publications

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“…Indeed, combined treatments of hCG with estradiol may regulate the growth response of OEC cells through a mechanism involving IGF-I (173). This finding is further supported by a recent report proposing the possible association of IGF-I and intergrin ␤1 with anchorage-dependent and -independent growth of OSE cells by LH (170). In addition, FSH and hCG stimulated steady-state mRNA levels of KGF, HGF, and kit ligand in bovine OSE cells (136).…”

Section: B Proliferationsupporting

confidence: 85%

“…These studies were performed with highly purified or recombinant gonadotropins, indicating negligible contamination. Thus, the reason for the discrepancy in results is not known, but it may be that variations in hormone concentration or the culture density used in different studies could be affecting the outcomes (169,170,175). It is also possible that variations in the expression of the gonadotropin receptors or their downstream signaling molecules between cultures of OSE derived from different women may explain an inconsistent response to gonadotropins (165).…”

Section: B Proliferationmentioning

confidence: 77%

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Gonadotropins and Ovarian Cancer

et al. 2007

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Ovarian epithelial cancer (OEC) accounts for 90% of all ovarian cancers and is the leading cause of death from gynecological cancers in North America and Europe. Despite its clinical significance, the factors that regulate the development and progression of ovarian cancer are among the least understood of all major human malignancies. The two gonadotropins, FSH and LH, are key regulators of ovarian cell functions, and the potential role of gonadotropins in the pathogenesis of ovarian cancer is suggested. Ovarian carcinomas have been found to express specific receptors for gonadotropins. The presence of gonadotropins in ovarian tumor fluid suggests the importance of these factors in the transformation and progression of ovarian cancers as well as being prognostic indicators. Functionally, there is evidence showing a direct action of gonadotropins on ovarian tumor cell growth. This review summarizes the key findings and recent advances in our understanding of these peptide hormones in ovarian cancer development and progression and their role in potential future cancer therapy. We will first discuss the supporting evidence and controversies in the "gonadotropin theory" and the use of animal models for exploring the involvement of gonadotropins in the etiology of ovarian cancer. The role of gonadotropins in regulating the proliferation, survival, and metastasis of OEC is next summarized. Relevant data from ovarian surface epithelium, which is widely believed to be the precursor of OEC, are also described. Finally, we will discuss the clinical applications of gonadotropins in ovarian cancer and the recent progress in drug development.

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Section: B Proliferationsupporting

confidence: 85%

Section: B Proliferationmentioning

confidence: 77%

Gonadotropins and Ovarian Cancer

et al. 2007

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“…Using the experimental system of Auersperg et al (1999), E-cadherin expression was recently addressed in human ovarian carcinogenesis (Ong et al, 2000). Furthermore, in the OSE cells, autocrine HGF-Met and LH-LH/hCG receptor loops have been shown to be involved in early carcinogenesis Tashiro et al, 2003). Although the system using the immortalised human OSE cell lines would serve as relevant models, the utility of the existing cell lines is limited by their accumulation of chromosomal instability.…”

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confidence: 99%

Establishment of an immortalised human ovarian surface epithelial cell line without chromosomal instability

et al. 2005

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Epithelial ovarian carcinoma is thought to derive from ovarian surface epithelium (OSE). The black box of the early molecular changes in ovarian carcinogenesis is being interpreted by the development of experimental systems employing immortalised human OSE cells. However, the existing cell lines of the OSE cells have limited utility due to chromosomal instability. Our goal was to establish new immortalised human OSE cells that retain the original characteristics of the primary cells without chromosomal alterations. Using primary human OSE cells obtained from a postmenopausal patient with endometrial cancer, five cell lines ('HOSE1' lines) were newly established by infection with retroviral expression vectors containing type 16 human papillomavirus (HPV-16) E6, E7, a variant E6 (E6D151), and Bmi1 polycomb gene, in combination with telomerase reverse transcriptase (hTERT). Consequently, five HOSE1s cell lines, HOSE1s-E6/hTERT, -E7/hTERT, -E6/E7/hTERT, -E6D151/E7/hTERT, and -E6D151/Bmi1/hTERT, grew beyond the population doubling number of 200. These cell lines, except for HOSE1-E6/hTERT, essentially showed the original features of the primary human OSE cells. Of them, HOSE1-E7/hTERT preserved diploidy in a kariotype analysis, and did not show transformed phenotypes in anchorage-independent growth and tumour formation. Thus, HOSE1-E7/hTERT may provide a novel model system with which to investigate the mechanisms of early molecular changes.

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“…Індукована гіперекспресія ре-цептора до ФСГ веде до підвищення експресії рецепторів епідермального фактора росту (epidermal growth factor receptor -EGFR), ре-цептора типу 2 до людського епідермального фактора росту (human epidermal growth factor receptor 2 -HER2) [12]. До інших можливих онкогенів, експресія яких підвищується за дії ФСГ і лГ in vitro, належать β-катенін, Meis-1, циклін G2, інсуліноподібний фактор росту 1 і інтегрин β-1 [13].…”

Section: гормонозалежність раку яєчниківunclassified