Roles of membrane protein damage and intracellular protein damage in death of bacteria induced by atmospheric-pressure air discharge plasmas

Abstract

Although plasma sterilization has attracted much attention, the underlying mechanisms and biochemical pathways are still not fully understood.

Uncovering the inactivation kinetics of Escherichia coli in saline by atmospheric DBD plasma using ATR FT‐IR

“…This is supported by a work of Hao Zhang et al It is found that α‐helix content decreases and β‐sheet increases in both membrane and intracellular proteins with regard to after‐charge storage. [ 30 ] Moreover, the amide I/II intensity ratio is a good indicator of protein secondary structures. [ 48 ] In this study, this ratio increased monotonically with treatment time (1.49, 1.52, 1.56, and 1.63 for 0, 60, 90, and 120 s of exposure time, respectively).…”

“…[ 27 ] In other research studies, various biological effects of plasma on bacteria are investigated. In summary, membrane integrity loss, [ 25,28 ] lipid peroxidation, [ 25,28,29 ] protein oxidative modifications, [ 16 ] changes in protein secondary or/and tertiary structures, [ 30 ] oxidative DNA damages, [ 28,31 ] and protein–DNA crosslink [ 32 ] are observed in treated bacterial solutions. Nevertheless, more efforts are required to clarify the effects of plasma on macromolecules in bacteria.…”

The Antitumor Effects of Plasma-Activated Saline on Muscle-Invasive Bladder Cancer Cells In Vitro and In Vivo Demonstrate Its Feasibility as a Potential Therapeutic Approach

“…Plasma-irradiated solutions with anticancer effects are termed plasma-activated solutions [ 33 ]. It is considered that, during CAP irradiation, the solutions were enriched with a wide variety of aqueous ROS and RNS, such as hydrogen peroxide (H 2 O 2 ), hydroxide radicals (OH•), superoxide anion radicals (O 2 − ), ozone (O 3 ), nitrite ions (NO 2 − ), peroxynitrite (ONOOH/ONOO − ), and nitrate ions (NO 3 − ) [ 33 , 34 , 35 , 36 , 37 , 38 , 39 ]. These aqueous ROS and RNS can trigger intracellular oxidative stress, mitochondrial dysfunction and DNA damage, and further activate the related signaling pathways of programmed cell death [ 23 , 40 , 41 , 42 , 43 ].…”

Synergistic anticancer effects of different combinations of He+O2 plasma jet and doxorubicin on A375 melanoma cells

“…[ 12–16 ] When cells are treated with CAP, these gas‐phase reactive species react with aqueous solutions to produce a rich variety of liquid‐phase reactive species, such as reactive oxygen species (ROS) and reactive nitrogen species (RNS). [ 17–21 ] These liquid‐phase reactive species can act on cells and induce an increase in intracellular ROS levels. [ 3,20,22–24 ] Cells also accumulate ROS and other byproducts during normal oxidative respiration and metabolism.…”