Roles of methanogens on volatile organic sulfur compound production in anaerobically digested wastewater biosolids

Chen, Yen-Chih; Higgins, Matthew J.; Maas, Nick A.; Murthy, Sudhir; Toffey, William E.; Foster, D. J.

doi:10.2166/wst.2005.0499

Cited by 34 publications

(31 citation statements)

References 5 publications

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“…Our results indicate that both aerobic and anaerobic treatments followed by storage were superior to storage alone for the reduction of total solids, BOD 5 , and coliform bacteria. In addition to these reductions, each system had unique remediation properties.…”

Section: Vol 73 2007mentioning

confidence: 73%

“…Fresh waste material was added into the feed tank weekly, and any material remaining in the feed tank was discarded when fresh material was added. The aerobic and anaerobic reactors were designed and operated to reduce total solids and biological oxygen demand (BOD 5 ) by approximately 35 and 80%, respectively, and are described schematically in Fig. 1.…”

Section: Methodsmentioning

confidence: 99%

“…In the oxygen-rich environment of the aerobic reactor, ammonia likely became nitrified by ammonia-oxidizing bacteria of the genus Nitrosomonas, whose 16S rRNA gene sequences were only observed in the aerobic treatment system (data not shown). When the oxidized nitrogen species entered the anoxic conditions of the storage tank, they were (3,5,6) and, to a lesser extent, by assimilation. At the phylum level, the feed material-derived 16S rRNA gene library was very similar to a library constructed from dairy waste reported previously (26).…”

Section: Vol 73 2007mentioning

confidence: 99%

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Bacterial Population Dynamics in Dairy Waste during Aerobic and Anaerobic Treatment and Subsequent Storage

McGarvey

Miller

Zhang³

et al. 2007

Appl Environ Microbiol

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The objective of this study was to model a typical dairy waste stream, monitor the chemical and bacterial population dynamics that occur during aerobic or anaerobic treatment and subsequent storage in a simulated lagoon, and compare them to those of waste held without treatment in a simulated lagoon. Both aerobic and anaerobic treatment methods followed by storage effectively reduced the levels of total solids (59 to 68%), biological oxygen demand (85 to 90%), and sulfate (56 to 65%), as well as aerobic (83 to 95%), anaerobic (80 to 90%), and coliform (>99%) bacteria. However, only aerobic treatment reduced the levels of ammonia, and anaerobic treatment was more effective at reducing total sulfur and sulfate. The bacterial population structure of waste before and after treatment was monitored using 16S rRNA gene sequence libraries. Both treatments had unique effects on the bacterial population structure of waste. Aerobic treatment resulted in the greatest change in the type of bacteria present, with the levels of eight out of nine phyla being significantly altered. The most notable differences were the >16-fold increase in the phylum Proteobacteria and the approximately 8-fold decrease in the phylum Firmicutes. Anaerobic treatment resulted in fewer alterations, but significant decreases in the phyla Actinobacteria and Bacteroidetes, and increases in the phyla Planctomycetes, Spirochetes, and TM7 were observed.

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Section: Vol 73 2007mentioning

confidence: 73%

Section: Methodsmentioning

confidence: 99%

Section: Vol 73 2007mentioning

confidence: 99%

See 1 more Smart Citation

Bacterial Population Dynamics in Dairy Waste during Aerobic and Anaerobic Treatment and Subsequent Storage

McGarvey

Miller

Zhang³

et al. 2007

Appl Environ Microbiol

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“…provided useful insight into methanogenic inhibition occurring at high digestion temperatures. In an anaerobic digester, analysis of total archaeal population is considered to be representative of the methanogenic population (Chen et al, 2005). Analysis of reactors 35°C, 49°C, and 53°C2 yielded the presence of distinct bands, representing individual populations of methanogenic organisms.…”

Section: Resultsmentioning

confidence: 99%

“…DGGE was carried out for bacterial and archaeal analyses at run times of 5.5 hours and 3.5 hours, respectively, and with an applied voltage of 200V as previously described (Nakatsu et al, 2000). Primers PRBA338f and PRUN518r were used for community analysis of organisms in the bacterial domain, and primers PARCH340f and PARCH519r were used for community analysis of the archaeal community (Chen et al, 2005).…”

Section: Pcr-dgge Analysismentioning

confidence: 99%

The effect of temperature on the performance and stability of thermophilic anaerobic digestion

Wilson

Murthy

Yuan

et al. 2008

Water Science and Technology

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Sustainable operation of an anaerobic sewage sludge digester requires the effective shuttling of carbon from complex organic material to methane gas. The accumulation of intermediates and metabolic products such as volatile fatty acids and hydrogen gas not only reveal inefficiency within the digestion process, but can be detrimental to reactor operation at sufficiently high levels. Eight anaerobic digesters (1 mesophilic and 7 thermophilic) were operated in order to determine the effect of steady-state digestion temperature on the operational stability and performance of the digestion process. Replicate reactors operated at 57.5°C, the highest temperature studied, were prone to accumulation of volatile fatty acids (4052 and 3411 mg/L as acetate) and gaseous hydrogen. Reactors operated at or below 55°C showed no such accumulation of intermediate metabolites. Overall methanogenesis was also greatly reduced at 57.5°C (0.09 L CH4/g VS fed) versus optimal methane formation at 53°C (0.40 L CH4/ g VS fed). Microbial community assessment and free energy calculations suggest that the accumulation of fatty acids and hydrogen, and relatively poor methanogenic performance at 57.5°C are likely due to temperature limitations of thermophilic aceticlastic methanogens.Keywords: Acetate; methane; hydrogen; propionate; temperature; thermophilic anaerobic digestion INTRODUCTION Stringent pathogen regulations required for the land application of domestic wastewater biosolids have revitalized interest in thermophilic anaerobic digestion. Since biological activity at these temperatures requires specialized organisms equipped with thermostable cell components and enzymes, thermophilic anaerobic digestion has recently been recognized as holistically unique biological process, rather than a close variation of mesophilic anaerobic digestion. As such, much of the fundamental discussion regarding anaerobic digestion technology, keeping mesophilic anaerobic digestion in mind, is inadequate for the development of a thorough understanding of the design parameters and operational variables that greatly affect thermophilic performance.

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Creating topsoils and soil conditioners from biosolids and urban residuals

Batjiaka

Brown

2020

J of Env Quality

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A study was conducted to test the suitability of a range of organic and inorganic residuals mixed with municipal biosolids to create a soil amendment effective for a broad range of end uses. Biosolids suitable for unrestricted use were sourced from San Francisco, CA. Residuals including urban wood and yard waste, nut shells, biochar, and sawdust were sourced from nearby producers. Existing biosolids based soil products, peat, and a yard–food compost were used as controls. Experimental mixtures were tested for a range of soil properties, appearance, odor, germination, and growth response. Several mixtures performed as well as or better than the yard–food compost, and several performed comparably to the biosolids soil controls. Yard waste fines blended with biosolids in a 50:50 ratio by volume or yard waste fines blended with biosolids and 20% sand at 40:40:20 were highly effective across all measured indices. Mixed yard and recycled lumber fines blended with biosolids at these same ratios performed similarly well. Blends with a high percentage of char performed poorly in germination and growth response, likely as a result of elevated electrical conductivity. Certain blends such as gypsum and redwood shavings failed to mix well with biosolids to create a uniform looking material and were less visually appealing. In general, blends that met established quality control parameters for compost performed well on the measured indices. These results suggest that blending Class A biosolids with organic and inorganic feedstocks to meet quality criteria developed for compost will create marketable soil products.

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Roles of methanogens on volatile organic sulfur compound production in anaerobically digested wastewater biosolids

Cited by 34 publications

References 5 publications

Bacterial Population Dynamics in Dairy Waste during Aerobic and Anaerobic Treatment and Subsequent Storage

Bacterial Population Dynamics in Dairy Waste during Aerobic and Anaerobic Treatment and Subsequent Storage

The effect of temperature on the performance and stability of thermophilic anaerobic digestion

Creating topsoils and soil conditioners from biosolids and urban residuals

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