Roles of Mir-200 Family Members in Lung Cancer: More Than Tumor Suppressors

Liu, Cong; Hu, Wei; Li, Linlin; Wang, Yuxuan; Zhou, Qun; Zhang, Feng; Songyang, Yiyan; Zhu, Wei; Sun, Chengcao; Li, Dejia

doi:10.2217/fon-2018-0155

Cited by 49 publications

(39 citation statements)

References 98 publications

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“…25–28 MALAT1 and PD-L1 promote NSCLC progression, while multiple miR-200 family members play a role in fighting it. 29–36 Consistent with previous reports, we found that MALAT1 and PD-L1 were highly expressed in NSCLC, while miR-200a-3p was significantly downregulated. The starBase and TargetScan suggested that miR-200a-3p has possible binding sites of MALAT1, and PD-L1 was also one of the downstream targets of miR-200a-3p.…”

Section: Discussionsupporting

confidence: 91%

LncRNA MALAT1 contributes to non-small cell lung cancer progression via modulating miR-200a-3p/programmed death-ligand 1 axis

Wei

Wang

Huang

et al. 2019

Int J Immunopathol Pharmacol

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This study was to investigate the expression correlation between long non-coding RNA metastasis-associated lung adenocarcinoma transcript 1 (lncRNA MALAT1), miR-200a-3p and programmed death-ligand 1 (PD-L1) in non-small cell lung cancer (NSCLC), and their roles in NSCLC. Real-time polymerase chain reaction (PCR) was performed to detect the expressions of MALAT1, miR-200a-3p and PD-L1 in NSCLC tissues and cells for the correlation analysis. The starBase and Targetscan databases were used to predict the binding sites between MALAT1 and miR-200a-3p, and miR-200a-3p and PD-L1, respectively. The targeting relationship between MALAT1 and miR-200a-3p, and miR-200a-3p and PD-L1 were further verified by real-time PCR and dual luciferase reporter gene assay. Cell proliferation was monitored by CCK8 and colony formation assays. The apoptosis was detected using flow cytometry. Wound healing assay and transwell assay were conducted to determine cell migration and invasion. In this study, we demonstrated that in NSCLC tissues, the expression level of MALAT1 was negatively correlated with that of miR-200a-3p, while positively correlated with PD-L1. Besides, MALAT1 promoted proliferation, mobility, migration, and invasion of NSCLC cells via sponging miR-200a-3p. PD-L1 was validated as a target of miR-200a-3p, and indirectly modulated by MALAT1. In conclusion, LncRNA MALAT1 facilitates the progression of NSCLC by modulating miR-200a-3p/PDL1 axis.

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Section: Discussionsupporting

confidence: 91%

LncRNA MALAT1 contributes to non-small cell lung cancer progression via modulating miR-200a-3p/programmed death-ligand 1 axis

Wei

Wang

Huang

et al. 2019

Int J Immunopathol Pharmacol

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“…The miR-200 family targets the expression of many genes, including ZEBs (Zinc finger E-boxbinding homeobox), which are the transcription factors that regulate cellular transformation, more specifically epithelial-to-mesenchymal transition (EMT), during cancer development and progression through repression of adhesion molecules such as E-cadherin [51]. Members of the miR-200 family also host diagnostic and prognostic potential in other cancer sites such as gastric, ovarian, lung and colorectal cancer [52][53][54][55].…”

Section: Discussionmentioning

confidence: 99%

Diagnostic value of microRNA panel in endometrial cancer: A systematic review

2020

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Purpose: We conducted a systematic review to evaluate the overall diagnostic accuracy of miRNAs in detecting endometrial cancer. Materials and Methods: A systematic search of Medline, Embase, Cinahl and the Cochrane Controlled Register of Trials was performed to identify studies reporting on the diagnostic value of miRNA in EC patients. Included were diagnostic studies looking at miRNA expression in women diagnosed with endometrial cancer. Two reviewers independently selected studies and assessed quality of studies using the Quality Assessment of Diagnostic Accuracy Studies 2 (QUADAS-2) score system. Data extraction was completed and the vote-counting strategy was used to rank miRNAs. Results: 26 studies were included with a total number of 1,400 EC patients reporting on 106 differentially expressed miRNAs. The most frequently found upregulated miRNA was miR-205 followed by miR-200c,-223,-182,-183 and-200a. In addition, miR-135b, miR-429, miR-141 and miR-200b were also frequently upregulated. There was less consensus on down-regulated miRNAs. Conclusions: miRNAs yield a promising diagnostic biomarker potential in endometrial cancer, especially miR-205, the miR-200 family and miR-135b,-182,-183 and-223. However, no sufficient high quality data are available to draw hard conclusions. More research is needed to validate the diagnostic potential of these miRNAs in larger studies. In addition, the potential of urine as a non-invasive biofluid should be investigated in more detail.

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“…40 Recent study also demonstrated that miR-200 family members suppressed the epithelial-mesenchymal transition of lung cancer. 41 The tumor suppressive role of miR-200 family is closely associated with the glinoma initiation, progression and metastasis. 42 These findings suggested the opposite function of miR-200a-3p and other miR-200 family members in cancers.…”

Section: Discussionmentioning

confidence: 99%

<p>MiR-200a-3p promoted the malignant behaviors of ovarian cancer cells through regulating PCDH9</p>

Shi¹,

Yang²,

Zhang³

et al. 2019

OTT

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Background: Increasing evidence has revealed that the aberrant expression of microRNAs (miRNAs) plays vital roles in the development and progression of ovarian cancer. MiR-200a-3p was found to act as an oncogene in a variety of cancers, however, the expression and function of miR-200a-3p in ovarian cancer has not been characterized. Materials and methods: The expression of miR-200a-3p in ovarian cancer tissues and cell lines was detected by the RT-qPCR. The influence of miR-200a-3p on the growth of ovarian cancer cells was determined with the Cell Counting Kit-8 assay, colony formation and cell invasion assay. The binding of miR-200a-3p with the 3ʹ-untranslated region (UTR) of PDCH9 was detected by luciferase reporter assay. The expression of PCDH9 was investigated by RT-qPCR and Western blot analysis. Results: miR-200a-3p was up-regulated in ovarian cancer tissues and cell lines. Highly expressed miR-200a-3p was significantly associated with the tumor size, tumor metastasis and TNM stage. Overexpression of miR-200a-3p markedly promoted the proliferation, colony formation and invasion of ovarian cancer cells. Functional study uncovered that miR-200a-3p bound the 3ʹ-untranslated region (UTR) of PCDH9 and decreased the expression of PCDH9 in ovarian cancer cells. The expression of miR-200a-3p in ovarian cancer tissues was significantly negatively correlated with that of PCDH9. Restored PCDH9 inhibited the promoting effect of miR-200a-3p on the proliferation of ovarian cancer cells. Conclusion: Our results suggested the potential oncogenic function of miR-200a-3p via modulating PCDH9 in ovarian cancer.

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Roles of Mir-200 Family Members in Lung Cancer: More Than Tumor Suppressors

Cited by 49 publications

References 98 publications

LncRNA MALAT1 contributes to non-small cell lung cancer progression via modulating miR-200a-3p/programmed death-ligand 1 axis

LncRNA MALAT1 contributes to non-small cell lung cancer progression via modulating miR-200a-3p/programmed death-ligand 1 axis

Diagnostic value of microRNA panel in endometrial cancer: A systematic review

<p>MiR-200a-3p promoted the malignant behaviors of ovarian cancer cells through regulating PCDH9</p>

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