Roles of miR-432 and circ_0000418 in mediating the anti-depressant action of ADAR1

Zhang, Xiaonan; Yan, Wei; Xue, Ying; Xu, Hong; Li, Jinying; Zhao, Ziwei; Sun, Ying; Wang, Yanfang; He, Jiaqian; Huang, Yuyue; Yu, Dao‐Yi; Xiao, Zhaoyang; Yin, Shanshan

doi:10.1016/j.ynstr.2021.100396

Cited by 4 publications

(10 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In participants with MDD and ANX, miR-432-5p was downregulated; miR-432-5p regulates the expression of ADAR1 , which is suggested to be involved in processes associated with MDD [ 43 ]. ADAR1 is highly expressed in brain, and it is involved in pre-mRNA editing through deamination of adenosine.…”

Section: Discussionmentioning

confidence: 99%

Alterations in microRNA of extracellular vesicles associated with major depression, attention-deficit/hyperactivity and anxiety disorders in adolescents

et al. 2023

View full text Add to dashboard Cite

Extracellular vesicles (EVs) are present in numerous peripheral bodily fluids and function in critical biological processes, including cell-to-cell communication. Most relevant to the present study, EVs contain microRNAs (miRNAs), and initial evidence from the field indicates that miRNAs detected in circulating EVs have been previously associated with mental health disorders. Here, we conducted an exploratory longitudinal and cross-sectional analysis of miRNA expression in serum EVs from adolescent participants. We analyzed data from a larger ongoing cohort study, evaluating 116 adolescent participants at two time points (wave 1 and wave 2) separated by three years. Two separate data analyses were employed: A cross-sectional analysis compared individuals diagnosed with Major Depressive Disorder (MDD), Anxiety disorders (ANX) and Attention deficit/Hyperactivity disorder (ADHD) with individuals without psychiatric diagnosis at each time point. A longitudinal analysis assessed changes in miRNA expression over time between four groups showing different diagnostic trajectories (persistent diagnosis, first incidence, remitted and typically developing/control). Total EVs were isolated, characterized by size distribution and membrane proteins, and miRNAs were isolated and sequenced. We then selected differentially expressed miRNAs for target prediction and pathway enrichment analysis. In the longitudinal analysis, we did not observe any statistically significant results. In the cross-sectional analysis: in the ADHD group, we observed an upregulation of miR-328-3p at wave 1 only; in the MDD group, we observed a downregulation of miR-4433b-5p, miR-584-5p, miR-625-3p, miR-432-5p and miR-409-3p at wave 2 only; and in the ANX group, we observed a downregulation of miR-432-5p, miR-151a-5p and miR-584-5p in ANX cases at wave 2 only. Our results identified previously observed and novel differentially expressed miRNAs and their relationship with three mental health disorders. These data are consistent with the notion that these miRNAs might regulate the expression of genes associated with these traits in genome-wide association studies. The findings support the promise of continued identification of miRNAs contained within peripheral EVs as biomarkers for mental health disorders.

show abstract

Section: Discussionmentioning

confidence: 99%

Alterations in microRNA of extracellular vesicles associated with major depression, attention-deficit/hyperactivity and anxiety disorders in adolescents

et al. 2023

View full text Add to dashboard Cite

show abstract

“…However, so far, the detailed molecular mechanism of stress-induced cognitive impairment has not been fully clarified. Environmental stress exposures affect animal behavior via the epigenetic regulation mechanism ( Chen et al, 2016 ; Yu et al, 2018 ; Zhang et al, 2021a ; Zhang et al, 2021b ), which provides a new idea to reveal the mechanism of stress-induced cognitive impairment.…”

Section: Discussionmentioning

confidence: 99%

“…The expression of ADAR1 in the frontal cortex and hippocampus of BALB/c mice exposed to chronic unpredictable stressors decreased significantly. After administration of ADAR1 inducer, the reduced expression of ADAR1 protein in the brain is recovered ( Zhang et al, 2021a ; Zhang et al, 2021b ). In the mammalian genome, the ADAR family has three members: ADAR1, ADAR2, and ADAR3.…”

Section: Discussionmentioning

confidence: 99%

“…ADAR1 plays an important role in maintaining the normal function of the nervous and immune systems ( Veno et al, 2012 ; Nakahama and Kawahara, 2020 ). It has been reported that ADAR1 is involved in the pathogenesis of human diseases by editing and non-editing actions ( Song et al, 2016 ; Gallo et al, 2017 ), including tumors ( Nemlich Y et al, 2013 ; Han et al, 2015 ; Paz-Yaacov et al, 2015 ; Anadon et al, 2016 ; Chan et al, 2016 ), autoimmune diseases ( Nakahama and Kawahara, 2020 ), cardiovascular diseases ( Stellos et al, 2016 ), and mental diseases ( Simmons et al, 2010 ; Kunii et al, 2011 ; Zhang et al, 2021a ; Zhang et al, 2021b ). Environmental stressors change the A-I editing mode catalyzed by ADAR1 ( Simmons et al, 2010 ; Karanovic et al, 2015 ), thus affecting the expression of downstream target proteins.…”

Section: Discussionmentioning

confidence: 99%

“…Environmental stressors change the A-I editing mode catalyzed by ADAR1 ( Simmons et al, 2010 ; Karanovic et al, 2015 ), thus affecting the expression of downstream target proteins. ADAR1 affects BDNF expression of the frontal cortex and hippocampus by acting on miR-432 and circ_0000418 in chronic unpredictable stress-treated mice ( Zhang et al, 2021b ). However, the mechanism of ADAR1 in chronic stress-induced cognitive dysfunction is still unclear.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

The involvement of ADAR1 in chronic unpredictable stress-induced cognitive impairment by targeting DARPP-32 with miR-874-3p in BALB/c mice

Wang

Liu

Zhao

et al. 2023

Front. Cell Dev. Biol.

Self Cite

View full text Add to dashboard Cite

Introduction: Chronic stress exposure is the main environmental factor leading to cognitive impairment, but the detailed molecular mechanism is still unclear. Adenosine Deaminase acting on double-stranded RNA1(ADAR1) is involved in the occurrence of chronic stress-induced cognitive impairment. In addition, dopamine and Adenosine 3′5′-monophosphate-regulated phospho-protein (DARPP-32) gene variation affects cognitive function. Therefore, we hypothesized that ADAR1 plays a key role in chronic stress-induced cognitive impairment by acting on DARPP-32.Methods: In this study, postnatal 21-day-old male BALB/c mice were exposed to chronic unpredictable stressors. After that, the mice were treated with ADAR1 inducer/inhibitor. The cognitive ability and cerebral DARPP-32 protein expression of BALB/c mice were evaluated. In order to explore the link between ADAR1 and DARPP-32, the effects of ADAR1 high/low expression on DARPP-32 protein expression in vitro were detected.Results: ADAR1 inducer alleviates cognitive impairment and recovers decreased DARPP-32 protein expression of the hippocampus and prefrontal cortex in BALB/c mice with chronic unpredictable stress exposure. In vivo and in vitro studies confirm the results predicted by bio-informatics; that is, ADAR1 affects DARPP-32 expression via miR-874-3p.Discussion: The results in this study demonstrate that ADAR1 affects the expression of DARPP-32 via miR-874-3p, which is involved in the molecular mechanism of pathogenesis in chronic unpredictable stress-induced cognitive impairment. The new findings of this study provide a new therapeutic strategy for the prevention and treatment of stress cognitive impairment from epigenetics.

show abstract

Hsa_circ_0000418 promotes the progression of glioma by regulating microRNA-409-3p / pyruvate dehydrogenase kinase 1 axis

Chen²,

Zhou

et al. 2022

Bioengineered

View full text Add to dashboard Cite

Roles of miR-432 and circ_0000418 in mediating the anti-depressant action of ADAR1

Cited by 4 publications

References 52 publications

Alterations in microRNA of extracellular vesicles associated with major depression, attention-deficit/hyperactivity and anxiety disorders in adolescents

Alterations in microRNA of extracellular vesicles associated with major depression, attention-deficit/hyperactivity and anxiety disorders in adolescents

The involvement of ADAR1 in chronic unpredictable stress-induced cognitive impairment by targeting DARPP-32 with miR-874-3p in BALB/c mice

Hsa_circ_0000418 promotes the progression of glioma by regulating microRNA-409-3p / pyruvate dehydrogenase kinase 1 axis

Contact Info

Product

Resources

About