Roles of miR-640 and Zinc Finger Protein 91 (ZFP91) in Angiopoietin-1-Induced In Vitro Angiogenesis

Harel, Sharon; Sanchez‐Gonzalez, Veronica; Echavarría, Raquel; Mayaki, Dominique; Hussain, Sabah N. A.

doi:10.3390/cells9071602

Cited by 15 publications

(10 citation statements)

References 47 publications

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“…These products including sonic hedgehog homolog (SHH) ( Weyers et al, 2020 ), HIF-1 ( Chai et al, 2020 ; Coyle et al, 2020 ), and BMP ( Samee et al, 2008 ; Yang et al, 2021 ), which recruit cells in the vascularization area to secret angiogenic factors. PDGF, angiopoietin 1, and ephrinB2 also show an indirect role on angiogenesis ( Harel et al, 2020 ; Hosaka et al, 2020 ; Yoon et al, 2020 ). They may help to stabilize the newly formed capillaries by recruiting smooth muscle cells.…”

Section: Co-culture System Optimizationmentioning

confidence: 99%

Recent Advances on Cell-Based Co-Culture Strategies for Prevascularization in Tissue Engineering

Shafiee

Shariatzadeh

Zafari

et al. 2021

Front. Bioeng. Biotechnol.

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Currently, the fabrication of a functional vascular network to maintain the viability of engineered tissues is a major bottleneck in the way of developing a more advanced engineered construct. Inspired by vasculogenesis during the embryonic period, the in vitro prevascularization strategies have focused on optimizing communications and interactions of cells, biomaterial and culture conditions to develop a capillary-like network to tackle the aforementioned issue. Many of these studies employ a combination of endothelial lineage cells and supporting cells such as mesenchymal stem cells, fibroblasts, and perivascular cells to create a lumenized endothelial network. These supporting cells are necessary for the stabilization of the newly developed endothelial network. Moreover, to optimize endothelial network development without impairing biomechanical properties of scaffolds or differentiation of target tissue cells, several other factors, including target tissue, endothelial cell origins, the choice of supporting cell, culture condition, incorporated pro-angiogenic factors, and choice of biomaterial must be taken into account. The prevascularization method can also influence the endothelial lineage cell/supporting cell co-culture system to vascularize the bioengineered constructs. This review aims to investigate the recent advances on standard cells used in in vitro prevascularization methods, their co-culture systems, and conditions in which they form an organized and functional vascular network.

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Section: Co-culture System Optimizationmentioning

confidence: 99%

Recent Advances on Cell-Based Co-Culture Strategies for Prevascularization in Tissue Engineering

Shafiee

Shariatzadeh

Zafari

et al. 2021

Front. Bioeng. Biotechnol.

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“…Despite the discovery of several miRNAs associated with the development and progression of glioma (16)(17)(18), researchers are yet to investigate the effects of other miRNAs on glioblastoma cells (19)(20)(21). For example, miR-640 inhibits the formation of capillaries (22); however, its role in cancer development has not yet been investigated. In their research, Li et al (23) and Zhai et al (24) both demonstrated that miR-640 exerts tumor-suppressive effects in ovarian cancer and hepatocellular carcinoma.…”

Section: Introductionmentioning

confidence: 99%

MicroRNA‑640 promotes cell proliferation and adhesion in glioblastoma by targeting Slit guidance ligand 1

Luo

Chen

et al. 2020

Oncol Lett

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The effects of microRNAs (miRNAs/miRs) on glioblastoma have attracted the attention of researchers in the last 7 years. However, the role of miR-640 and its targeted gene, Slit guidance ligand 1 (SLIT1), in the development of glioblastoma are not yet fully understood. The present study aimed to investigate the role of miR-640 in the proliferation and adhesion of glioblastoma. Reverse transcription-quantitative PCR analysis was performed to detect miR-640 and SLIT1 expression in glioblastoma tissues and cells. In addition, the Dual-luciferase reporter and RNA-pull down assays were performed to assess the association between miR-640 and SLIT1. The Cell Counting Kit-8, BrdU ELISA, cell adhesion and caspase-3 activity assays were also performed to assess cell viability, proliferation, adhesion and apoptosis of glioblastoma cells, respectively. The results demonstrated that miR-640 expression was upregulated in glioblastoma tissues and cells. In addition, miR-640 promoted the cell viability, proliferation and adhesion of glioblastoma cells, while inhibiting cell apoptosis. SLIT1, a direct downstream target of miR-640, was demonstrated to be downregulated in glioblastoma tissues and cells. Furthermore, overexpression of SLIT1 attenuated the promotive effect of miR-640 on glioblastoma cells. Taken together, these results suggest that miR-640 accelerates the proliferation and adhesion of glioblastoma cell lines by targeting and suppressing SLIT1.

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“…There were 43 candidate miRNA targets of circ_0068087 predicted by both databases ( Supplementary Figure 2A ). Among these miRNAs, we screened out five miRNAs that were implicated in AS progression, including miR-186-5p ( 13 ), miR-140-3p ( 20 ), miR-640 ( 21 ), miR-515-5p ( 22 ), and miR-665 ( 23 ). MiR-186-5p was selected for further experiments due to it having the most significant negative regulatory relationship with circ_0068087 ( Supplementary Figure 2A ).…”

Section: Resultsmentioning

confidence: 99%

Circ_0068087 Silencing Ameliorates Oxidized Low-Density Lipoprotein-Induced Dysfunction in Vascular Endothelial Cells Depending on miR-186-5p-Mediated Regulation of Roundabout Guidance Receptor 1

Huang

Qin

et al. 2021

Front. Cardiovasc. Med.

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Background: Circular RNAs (circRNAs) are endogenous non-coding RNAs involved in the progression of atherosclerosis (AS). We investigated the role of circ_0068087 in AS progression and its associated mechanism.Methods: The 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) assay, flow cytometry, and enzyme-linked immunosorbent assay (ELISA) were performed to analyze the viability, apoptosis, and inflammatory response of HUVECs, respectively. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and the Western blot assay were performed to measure the expression of RNA and protein. Cell oxidative stress was analyzed using commercial kits. The dual-luciferase reporter assay and RNA immunoprecipitation (RIP) assay were conducted to verify the interaction between microRNA-186-5p (miR-186-5p) and circ_0068087 or roundabout guidance receptor 1 (ROBO1).Results: Oxidized low-density lipoprotein (ox-LDL) exposure upregulated the circ_0068087 level in HUVECs. ox-LDL-induced dysfunction in HUVECs was largely attenuated by the silence of circ_0068087. Circ_0068087 negatively regulated the miR-186-5p level by interacting with it in HUVECs. Circ_0068087 knockdown restrained ox-LDL-induced injury in HUVECs partly by upregulating miR-186-5p. ROBO1 was a downstream target of miR-186-5p in HUVECs. Circ_0068087 positively regulated ROBO1 expression by sponging miR-186-5p in HUVECs. MiR-186-5p overexpression exerted a protective role in ox-LDL-induced HUVECs partly by downregulating ROBO1.Conclusion: Circ_0068087 interference alleviated ox-LDL-induced dysfunction in HUVECs partly by reducing ROBO1 expression via upregulating miR-186-5p.

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Roles of miR-640 and Zinc Finger Protein 91 (ZFP91) in Angiopoietin-1-Induced In Vitro Angiogenesis

Cited by 15 publications

References 47 publications

Recent Advances on Cell-Based Co-Culture Strategies for Prevascularization in Tissue Engineering

Recent Advances on Cell-Based Co-Culture Strategies for Prevascularization in Tissue Engineering

MicroRNA‑640 promotes cell proliferation and adhesion in glioblastoma by targeting Slit guidance ligand 1

Circ_0068087 Silencing Ameliorates Oxidized Low-Density Lipoprotein-Induced Dysfunction in Vascular Endothelial Cells Depending on miR-186-5p-Mediated Regulation of Roundabout Guidance Receptor 1

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