Roles of Multidrug Transporters of MFS in Plant Stress Responses

Hua, Peng; Su-fen, Han; Luo, Mao; Li, Xuan; Zhao, Maojun

doi:10.7763/ijbbb.2011.v1.20

Cited by 12 publications

(10 citation statements)

References 52 publications

(41 reference statements)

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“…Here we validated expression of three salinity-responsive genes using RT-PCR. The five most important plant transporter families that have been implicated in biotic and abiotic stress responses included: ATP-binding cassette (ABC), multidrug and toxic compound exporters (MATE), major facilitator superfamily (MFS), small multidrug resistance (SMR), and resistance-nodulation-division proteins (RND) (Peng et al 2011). However, ABC is the largest transporter family protein found in all living organisms.…”

Section: Discussionmentioning

confidence: 99%

Comparative transcriptome profiling of upland (VS16) and lowland (AP13) ecotypes of switchgrass

et al. 2016

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Key message Transcriptomes of two switchgrass genotypes representing the upland and lowland ecotypes will be key tools in switchgrass genome annotation and biotic and abiotic stress functional genomics. AbstractSwitchgrass (Panicum virgatum L.) is an important bioenergy feedstock for cellulosic ethanol production. We report genome-wide transcriptome profiling of two contrasting tetraploid switchgrass genotypes, VS16 and AP13, representing the upland and lowland ecotypes, respectively. A total of 268 million Illumina short reads (50 nt) were generated, of which, 133 million were obtained in AP13 and the rest 135 million in VS16. More than 90% of these reads were mapped to the switchgrass reference genome (V1.1). We identified 6619 and 5369 differentially expressed genes in VS16 and AP13, respectively. Gene ontology and KEGG pathway analysis identified key genes that regulate important pathways including C4 photosynthesis, photorespiration and phenylpropanoid metabolism. A series of genes (33) involved in photosynthetic pathway were up-regulated in AP13 but only two genes showed higher expression in VS16. We identified three dicarboxylate transporter homologs that were highly expressed in AP13. Additionally, genes that mediate drought, heat, and salinity tolerance were also identified. Vesicular transport proteins, syntaxin and signal recognition particles were seen to be up-regulated in VS16. Analyses of selected genes involved in biosynthesis of secondary metabolites, plant–pathogen interaction, membrane transporters, heat, drought and salinity stress responses confirmed significant variation in the relative expression reflected in RNA-Seq data between VS16 and AP13 genotypes. The phenylpropanoid pathway genes identified here are potential targets for biofuel conversion.Electronic supplementary materialThe online version of this article (doi:10.1007/s00299-016-2065-0) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Comparative transcriptome profiling of upland (VS16) and lowland (AP13) ecotypes of switchgrass

et al. 2016

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“…Multidrug transporters play critical roles in plant defense, such as decreasing the accumulation of toxins secreted by the pathogen and exporting secondary metabolites out of the cell. 24 Ten transmembrane regions were predicted using TMHMM v.0.92b 25 for all sequences in this cluster and eight out of ten sites identified by MEME were found in the transmembrane regions (Fig. 3).…”

Section: Introductionmentioning

confidence: 99%

Identification of positive selection in disease response genes within members of the Poaceae

Rech

Vargas²,

Sukno

et al. 2012

Plant Signaling & Behavior

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“…They transport a wide spectrum of substrates across bio‐membranes and play a pivotal role in multiple physiological processes (Yan, ). In plants, MFS transporters respond to plant stresses, such as defending against infection by pathogens and exporting toxic compounds and heavy metals (Peng et al ., ). In this study, we noticed that the gene expression level of the contig C17235 was higher in mycelium sampled in situ than in the mycelium cultivated in vitro in presence of nickel.…”

Section: Resultsmentioning

confidence: 97%

Evidence of nickel (Ni) efflux in Ni‐tolerant ectomycorhizal Pisolithus albus isolated from ultramafic soil

Majorel

Hannibal

Ducousso

et al. 2014

Environ Microbiol Rep

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Nickel (Ni)-tolerant ectomycorrhizal Pisolithus albus was isolated from extreme ultramafic soils that are naturally rich in heavy metals. This study aimed to identify the specific molecular mechanisms associated with the response of P. albus to nickel. In presence of high concentration of nickel, P. albus Ni-tolerant isolate showed a low basal accumulation of nickel in its fungal tissues and was able to perform a metal efflux mechanism. Three genes putatively involved in metal efflux were identified from the P. albus transcriptome, and their overexpression was confirmed in the mycelium that was cultivated in vitro in the presence of nickel and in fungal tissues that were sampled in situ. Cloning these genes in yeast provided significant advantages in terms of nickel tolerance (+ 31% Ni EC50) and growth (+ 83% μ) compared with controls. Furthermore, nickel efflux was also detected in the transformed yeast cells. Protein sequence analysis indicated that the genes encoded a P-type-ATPase, an ABC transporter and a major facilitator superfamily permease (MFS). This study sheds light on a global mechanism of metal efflux by P. albus cells that supports nickel tolerance. These specific responses to nickel might contribute to the fungal adaptation in ultramafic soil.

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Roles of Multidrug Transporters of MFS in Plant Stress Responses

Cited by 12 publications

References 52 publications

Comparative transcriptome profiling of upland (VS16) and lowland (AP13) ecotypes of switchgrass

Comparative transcriptome profiling of upland (VS16) and lowland (AP13) ecotypes of switchgrass

Identification of positive selection in disease response genes within members of the Poaceae

Evidence of nickel (Ni) efflux in Ni‐tolerant ectomycorhizal Pisolithus albus isolated from ultramafic soil

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