2002
DOI: 10.1006/prep.2001.1578
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Roles of NADPH-P450 Reductase and Apo- and Holo-Cytochrome b5 on Xenobiotic Oxidations Catalyzed by 12 Recombinant Human Cytochrome P450s Expressed in Membranes of Escherichia coli

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Cited by 231 publications
(221 citation statements)
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“…As reported previously (46,63,85), the presence of b 5 enhanced the steady-state k cat for coumarin 7-hydroxylation (Table I). One general hypothesis for the enhancement of P450 catalytic activities by b 5 is transfer of an electron from ferrous b 5 to the P450 FeO 2ϩ -substrate complex (70,71).…”
supporting
confidence: 88%
See 1 more Smart Citation
“…As reported previously (46,63,85), the presence of b 5 enhanced the steady-state k cat for coumarin 7-hydroxylation (Table I). One general hypothesis for the enhancement of P450 catalytic activities by b 5 is transfer of an electron from ferrous b 5 to the P450 FeO 2ϩ -substrate complex (70,71).…”
supporting
confidence: 88%
“…The b 5 experiments are complex and difficult to interpret, but our overall conclusion is that some electron transfer from ferrous b 5 to the P450 2A6 FeO 2 2ϩ -coumarin complex occurs but that this does not seem to be a particularly efficient process. In other work we have shown that P450 2A6-catalyzed coumarin 7-hydroxylation can be enhanced by apo-b 5 devoid of heme and precluding electron transfer (85). We conclude that part of the enhancement may be attributable to the electron transfer (Figs.…”
supporting
confidence: 48%
“…Because of the considerable molar excess of reductase over CYP3A4, the latter is anticipated to be completely complexed with the flavoprotein and the functional properties of the monooxygenase are expected to be largely insensitive to possible variations in CPR content between the batches. This conclusion is supported by the observation of Yamazaki et al that the incorporation of exogenous CPR into CYP3A4 Baculosomes does not affect the rate of testosterone 6β-hydroxylation [53]. It should be noted also that all experiments on BFC debenzylation with intact Baculosomes were done with the first batch (39195B), while BQ experiments and the experiments with BFC and enriched baculosomes were done with the second batch.…”
Section: Activity Measurementssupporting
confidence: 56%
“…Individual porcine CYP450 enzymes have been studied to determine how metabolic capabilities correlate with those found in humans and for studies in veterinary pharmacology (Juskevich, 1987;Anzenbacherová et al, 2005). In addition, the microsomal haemoprotein cytochrome b5A (CYB5A) has been shown to augment the activity of human CYP2E1 (hCYP2E1) towards chlorzoxazone (CLZ) hydroxylation by apparently acting as an electron donor (Yamazaki et al, 1996;Yamazaki et al, 2002;Wiercinska and Squires, 2010). CYB5 also interacts with a number of other CYP450 isoforms including CYP17A1 (Billen and Squires, 2009); a CYB5 knockout mouse model has a dramatically altered expression of drug metabolizing enzymes and low levels of testicular androgens (McLaughlin et al, 2010).…”
Section: Introductionmentioning
confidence: 99%