Roles of Optineurin and Extracellular Vesicles in Glaucomatous Retinal Cell Loss

Duan, Chaoye; Fan, Wanlin; Chen, Fei

doi:10.1007/s11596-023-2730-9

Cited by 2 publications

(2 citation statements)

References 40 publications

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“…In addition, one also cannot transfer results from the RGC-5 experiments to the 661W cell line or photoreceptor properties. Although a few researchers still use these cells [ 28 , 29 ], several journals, including IOVS , Experimental Eye Research and Molecular Vision , stopped publishing articles based on RGC-5-based experiments in the context of ganglion cell research [ 70 , 72 , 73 ]. There are now several successful approaches to culturing RGC, and although this is much more complex, these cells reflect the true phenotype of neuronal cells [ 74 , 75 , 76 ].…”

Section: Discussionmentioning

confidence: 99%

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A Case Study from the Past: “The RGC-5 vs. the 661W Cell Line: Similarities, Differences and Contradictions—Are They Really the Same?”

Hurst,

Attrodt,

Bartz-Schmidt

et al. 2023

IJMS

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In the pursuit of identifying the underlying pathways of ocular diseases, the use of cell lines such as (retinal ganglion cell-5) RGC-5 and 661W became a valuable tool, including pathologies like retinal degeneration and glaucoma. In 2001, the establishment of the RGC-5 cell line marked a significant breakthrough in glaucoma research. Over time, however, concerns arose about the true nature of RGC-5 cells, with conflicting findings in the literature regarding their identity as retinal ganglion cells or photoreceptor-like cells. This study aimed to address the controversy surrounding the RGC-5 cell line’s origin and properties by comparing it with the 661W cell line, a known cone photoreceptor model. Both cell lines were differentiated according to two prior published redifferentiation protocols under the same conditions using 500 nM of trichostatin A (TSA) and investigated for their morphological and neuronal marker properties. The results demonstrated that both cell lines are murine, and they exhibited distinct morphological and neuronal marker properties. Notably, the RGC-5 cells showed higher expression of the neuronal marker β-III tubulin and increased Thy-1-mRNA compared with the 661W cells, providing evidence of their different properties. The findings emphasize the importance of verifying the authenticity of cell lines used in ocular research and highlight the risks of contamination and altered cell properties.

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Section: Discussionmentioning

confidence: 99%

“…Therefore, not all of these papers are questionable. Furthermore, in some cases, RGC-5 cells are still used for different research questions [ 28 , 29 , 30 ].…”

Section: Introductionmentioning

confidence: 99%