Roles of oxidative stress and endoplasmic reticulum stress in selenium deficiency-induced apoptosis in chicken liver

Abstract

Oxidative stress and endoplasmic reticulum (ER) stress are involved in different types of stress-induced injuries. The aim of the present study was to evaluate the effect of Se deficiency on oxidative stress, ER stress and apoptosis in chicken livers. Chickens (1 day old, n = 180) were randomly divided into two groups: the L group [fed with a Se-deficient (Se 0.033 mg/kg) diet] and the control group [fed with a normal (Se 0.2 mg/kg) diet]. Factor-associated oxidative stress, catalase (CAT) activity, H2O2 production and the inhibition of hydroxyl radicals (·OH) in the chicken liver were determined on days 15, 25, 35, 45, 55 and 65, respectively. In addition, ER stress-related genes (GRP78, GRP94, ATF4, ATF6 and IRE) and apoptosis-related genes (caspase3 and Bcl-2) were examined by fluorescence quantitative PCR or western blot analysis. Apoptosis levels were also measured using ultrastructural observations and the TdT-mediated dUTP nick end labeling assay. The results showed that CAT activity and ·OH inhibition were decreased and that H2O2 production was increased in the low-Se group, which demonstrated that oxidative stress occurred in the chicken liver. The ER stress-related genes (GRP78, GRP94, ATF4, ATF6 and IRE) and the apoptosis-related gene caspase3 were increased (p < 0.05), while Bcl-2 was decreased (p < 0.05) by Se deficiency. In addition, apoptosis and ER lesions were observed by ultrastructural observations of the chicken liver in the low-Se group. The level of apoptosis and the number of apoptotic cells increased with time. These results indicated that the oxidative-ER stress pathway participates in Se deficiency-induced apoptosis in the chicken liver.

Comparative genomics of Clavibacter michiganensis subspecies, pathogens of important agricultural crops

“…These complicated processes involve acquisition, sequestration, and efflux of metal ions [51]. Selenium occurs naturally in the Earth’s crust; and at low concentration it is essential for living organisms [52]. Under aerobic conditions, this trace element exists as selenite and selenate, and at high levels these salts can be toxic and mutagenic to bacteria [52,53].…”

“…Selenium occurs naturally in the Earth’s crust; and at low concentration it is essential for living organisms [52]. Under aerobic conditions, this trace element exists as selenite and selenate, and at high levels these salts can be toxic and mutagenic to bacteria [52,53]. High selenite-resistant bacterial strains like Ralstonia metallidurans CH34 possess the ded A gene that regulatesselenite uptake [52,53].…”

“…Under aerobic conditions, this trace element exists as selenite and selenate, and at high levels these salts can be toxic and mutagenic to bacteria [52,53]. High selenite-resistant bacterial strains like Ralstonia metallidurans CH34 possess the ded A gene that regulatesselenite uptake [52,53]. Three ded A genes encoding the putative selenite transport protein (DedA) including various polyols permease components of the ABC transporters are present in each of the Clavibacter subspecies, suggesting that members of the species C .…”

Effect of Selenium Deficiency on Nitric Oxide and Heat Shock Proteins in Chicken Erythrocytes

“…It has been reported that Se deficiency induced oxidative stress, apoptosis, and inflammatory response, which were accompanied by the upregulation of typical injury biomarkers such as NO, caspase-3, NF-κB, and iNOS in different tissues and organs [27][28][29]. However, the effect of Se deficiency on chicken erythrocytes is not completely understood.…”

Treatment of steroid-induced osteonecrosis of the femoral head using porous Se@SiO2 nanocomposites to suppress reactive oxygen species

“…As an essential trace mineral22, selenium (Se) plays an important role in suppressing oxidative stress232425 and has been proved interrelationship with DNA damage and oxidative stress26. Se deficiency may induce oxidative stress and endoplasmic reticulum stress27 in some special cases (such as the use of ECMO)28. In contrast, Se’s essential interrelationship with coenzyme Q10 in cardiovascular diseases29 and its preventive effect in combination with vitamin E for prostate cancers30 suggested a great potential for the prevention of ONFH.…”