Roles of Planar Cell Polarity Signaling in Maturation of Neuronal Precursor Cells in the Postnatal Mouse Olfactory Bulb

Abstract

Neuronal precursor cells generated by stem cells in the subventricular zone (SVZ) migrate and differentiate into mature interneurons in the olfactory bulb (OB). The mechanisms responsible for the dynamic morphological changes in cells during this process are largely unknown. Wnt/planar cell polarity (PCP) signaling regulates various developmental events, including neuronal migration and neurite formation. Here, we studied the function of two components of the PCP pathway, Dishevelled2 and Van Gogh like-2, in the newborn neurons in the postnatal mouse OB. Electroporation-or lentivirus-mediated introduction of vectors carrying a knockdown or dominant-negative construct of these genes into the SVZ altered the distribution and dendrite formation of newborn neurons in the OB, suggesting that PCP signaling is involved in regulating the maturation of new neurons in the OB.

Speed control for neuronal migration in the postnatal brain by Gmip-mediated local inactivation of RhoA

“…Electroporations of P1-2 WT mice were performed as described previously 57,58 with some modifications. Briefly, mice were anaesthetized by nitrous oxide and isoflurane and fixed to the platform of a stereotaxic injection apparatus (David Kopf Instruments, Tujunga, CA) by a craniophore.…”

Heterogeneous distribution of doublecortin‐expressing cells surrounding the rostral migratory stream in the juvenile mouse

“…All antibodies used for immunohistochemistry experiments were commercially available and demonstrated the appropriate pattern of cellular morphology and distribution (Bordiuk, Smith, Morin, & Semënov, ; Brunne et al, ; Hirota et al, ; Murata et al, ; Wang et al, ). Details of the primary antibody are described in Table .…”

Subventricular zone progenitors in time and space: generating neuronal diversity

“…The activation of this signaling contributes to the activation of the dorsal TF Tbr2 (Azim et al, 2014 ). The knock down of the downstream players of Wnt/Planar cell polarity signaling, Dvl2 or Vangl2, leads to a specific decrease in the generation of superficial granular neurons (Hirota et al, 2012 ), that are typically generated in more dorsal parts of the SVZ (Figure 1D ; Merkle et al, 2007 ). It is still not clear at what point interfering with Wnt signaling leads to the generation of ventrally-generated interneuron subtypes, however, it seems clear that dorsal TFs in the SVZ are positively affected by this molecule.…”