Roles of Plant Glycine-Rich RNA-Binding Proteins in Development and Stress Responses

Ma, Liqun; Cheng, Ke; Li, Jinyan; Deng, Zhiqi; Zhang, Chunjiao; Zhu, Hongliang

doi:10.3390/ijms22115849

Cited by 27 publications

(17 citation statements)

References 79 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A hub gene from the blue module DUH022017 .1 encodes GR-RBP produced as cold shock response. These proteins were reported earlier in abiotic stresses response, especially cold, to regulate circadian clock, resulting in delayed flowering and reproductive development, particularly by affecting FLC, a repressor of MADS- box observed in Arabidopsis, barley, rice, cauliflower, tobacco, carrot, ( Steffen et al, 2019 ; Alptekin et al, 2021 ; Ma et al, 2021 ). Another hub gene DUH011830 .…”

Section: Discussionmentioning

confidence: 70%

Comprehensive Biochemical, Physiological, and Transcriptomic Analyses Provide Insights Into Floral Bud Dormancy in Rhododendron delavayi Franch

et al. 2022

View full text Add to dashboard Cite

Due to a scarcity of relevant data, the ornamental woody flower Rhododendron delavayi Franch. is examined in the current study for its low temperature-induced floral bud dormancy (late October–end December) aspect. This study used transcriptome data profiling and co-expression network analyses to identify the interplay between endogenous hormones and bud dormancy phases such as pre-dormancy, para-dormancy, endo-dormancy, eco-dormancy, and dormancy release. The biochemical and physiological assays revealed the significance of the abundance of phytohormones (abscisic acid, auxin, zeatin, and gibberellins), carbohydrate metabolism, oxidative species, and proteins (soluble proteins, proline, and malondialdehyde) in the regulatory mechanism of floral bud dormancy. The transcriptome sequencing generated 65,531 transcripts, out of which 504, 514, 307, and 240 expressed transcripts were mapped uniquely to pre-, para-, endo-, and eco-phases of dormancy, showing their roles in the stimulation of dormancy. The transcripts related to LEA29, PGM, SAUR family, RPL9e, ATRX, FLOWERING LOCUS T, SERK1, ABFs, ASR2, and GID1 were identified as potential structural genes involved in floral bud dormancy. The transcription factors, including Zinc fingers, CAD, MADS-box family, MYB, and MYC2, revealed their potential regulatory roles concerning floral bud dormancy. The gene co-expression analysis highlighted essential hub genes involved in cold stress adaptations encoding proteins, viz, SERPIN, HMA, PMEI, LEA_2, TRX, PSBT, and AMAT. We exposed the connection among low temperature-induced dormancy in floral buds, differentially expressed genes, and hub genes via strict screening steps to escalate the confidence in selected genes as being truly putative in the pathways regulating bud dormancy mechanism. The identified candidate genes may prove worthy of further in-depth studies on molecular mechanisms involved in floral bud dormancy of Rhododendron species.

show abstract

Section: Discussionmentioning

confidence: 70%

Comprehensive Biochemical, Physiological, and Transcriptomic Analyses Provide Insights Into Floral Bud Dormancy in Rhododendron delavayi Franch

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Plant GRPs, a superfamily of proteins with high content of glycine (20-70%) and conserved glycinecontaining structural motif with repetitive amino acid residues, could be classified into five main classes (Czolpinska and Rurek, 2018). GRPs of class IV, also called glycine-rich RNA-binding proteins (GR-RBPs), can be further divided into four subclasses (denoted IVa-IVd) (Czolpinska and Rurek, 2018;Ma et al, 2021). Only members of subclass IVd, such as Arabidopsis GRP7, GRP8, and RZ-1C, share homology with the subunits of hnRNPs and are involved in the regulation of AS (Wang and Brendel, 2004).…”

Section: Discussionmentioning

confidence: 99%

Alternative Splicing Regulation of Glycine-Rich Proteins via Target of Rapamycin-Reactive Oxygen Species Pathway in Arabidopsis Seedlings Upon Glucose Stress

Bai

Chen

et al. 2022

Front. Plant Sci.

View full text Add to dashboard Cite

Glucose can serve as both the source of energy and regulatory signaling molecule in plant. Due to the environmental and metabolic change, sugar levels could affect various developmental processes. High glucose environment is hardly conductive to the plant growth but cause development arrest. Increasing evidence indicate that alternative splicing (AS) plays a pivotal role in sugar signaling. However, the regulatory mechanism upon glucose stress remains unclear. The full-length transcriptomes were obtained from the samples of Arabidopsis seedlings with 3% glucose and mock treatment, using Oxford Nanopore sequencing technologies. Further analysis indicated that many genes involved in photosynthesis were significantly repressed and many genes involved in glycolysis, mitochondrial function, and the response to oxidative stress were activated. In total, 1,220 significantly differential alternative splicing (DAS) events related to 619 genes were identified, among which 75.74% belong to intron retention (IR). Notably, more than 20% of DAS events come from a large set of glycine-rich protein (GRP) family genes, such as GRP7, whose AS types mostly belong to IR. Besides the known productive GRP transcript isoforms, we identified a lot of splicing variants with diverse introns spliced in messenger RNA (mRNA) region coding the glycine-rich (GR) domain. The AS pattern of GRPs changed and particularly, the productive GRPs increased upon glucose stress. These ASs of GRP pre-mRNAs triggered by glucose stress could be abolished by AZD-8055, which is an ATP competitive inhibitor for the target of rapamycin (TOR) kinase but could be mimicked by H2O2. Additionally, AS pattern change of arginine/serine-rich splicing factor 31(RS31) via TOR pathway, which was previously described in response to light and sucrose signaling, was also induced in a similar manner by both glucose stress and reactive oxygen species (ROS). Here we conclude that (i) glucose stress suppresses photosynthesis and activates the glycolysis-mitochondria energy relay and ROS scavenging system; (ii) glucose stress triggers transcriptome-wide AS pattern changes including a large set of splicing factors, such as GRPs and RS31; (iii) high sugars regulate AS pattern change of both GRPs and RS31 via TOR-ROS pathway. The results from this study will deepen our understanding of the AS regulation mechanism in sugar signaling.

show abstract

“…Glutamate is not only a precursor for the synthesis of chlorophyll, g-aminobutyric acid, etc., but also regulates plant development by participating in physiological processes such as seed germination and pollen germination (Qiu et al, 2020). In addition, the glycine-rich binding proteins GR-RBPs have important roles in seed germination and in promoting fruit ripening (Ma et al, 2021). Overall, G. lemaneiformis meets its material requirements during reproductive development by upregulating the synthesis of amino acids such as Asp, Gly and Glu.…”

Section: Carbon Metabolism Changes During the Spore Formationmentioning

confidence: 99%

Transcriptomic analysis reveals the regulatory mechanism of tetraspore formation in Gracilariopsis lemaneiformis

et al. 2023

View full text Add to dashboard Cite

With the expansion of seaweed culture and changes in the global climate, large quantities of new seaweed germplasm are urgently needed. It is important to elucidate the process of reproductive development and its regulatory mechanism in seaweed. Gracilariopsis lemaneiformis (Rhodophyta) has an essential economic and ecological value, for example, as a raw material for agar extraction and abalone feed. Here, four phases (I to IV) of G. lemaneiformis tetrasporophyte development were analyzed using physiological assays and transcriptome technologies. The results showed that photosynthetic capacity increased during the period from tetraspore formation to the release (Phase II, III and IV). According to transcriptome results, the expression levels of genes associated with light harvesting, photosynthesis, and carbon fixation pathways were significantly upregulated during tetraspore formation and release. Meanwhile, the expression levels of genes encoding starch and trehalose synthesis enzymes in starch and sucrose metabolism were enhanced during tetraspore formation and release, suggesting that G. lemaneiformis requires more energy supply during reproductive development, and that trehalose-6-phosphate may also act as a signaling molecule to induce tetraspore formation. In addition, genes encoding antioxidant enzymes (APX, TRX, GR, TR, PRX, and CAT) were significantly upregulated during tetraspore formation. These results may help us to understand the transition from nutritional to reproductive development and the molecular mechanism of G. lemaneiformis tetrasporogenesis, which is vital for the development of new germplasm and promoting the growth of the seaweed culture industry.

show abstract

Roles of Plant Glycine-Rich RNA-Binding Proteins in Development and Stress Responses

Cited by 27 publications

References 79 publications

Comprehensive Biochemical, Physiological, and Transcriptomic Analyses Provide Insights Into Floral Bud Dormancy in Rhododendron delavayi Franch

Comprehensive Biochemical, Physiological, and Transcriptomic Analyses Provide Insights Into Floral Bud Dormancy in Rhododendron delavayi Franch

Alternative Splicing Regulation of Glycine-Rich Proteins via Target of Rapamycin-Reactive Oxygen Species Pathway in Arabidopsis Seedlings Upon Glucose Stress

Transcriptomic analysis reveals the regulatory mechanism of tetraspore formation in Gracilariopsis lemaneiformis

Contact Info

Product

Resources

About