Roles of PprA, IrrE, and RecA in the resistance of Deinococcus radiodurans to germicidal and environmentally relevant UV radiation

Bauermeister, Anja; Bentchikou, Esma; Moeller, Ralf; Rettberg, Petra

doi:10.1007/s00203-009-0522-7

Cited by 34 publications

(23 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The F 10 value for D. radiodurans (660 J/m 2 ; Bauermeister et al 2009) is around 6 times higher as the F 10 value of the tested (hyper-) thermophilic Archaea ( Table 2). The F 10 value of A. fulgidus is in the range of B. subtilis spores (100 J/m 2 ; Riesenman and Nicholson 2000), which is about twice the F 10 value of vegetative B. subtilis cells (40 J/m 2 ; Newcombe et al 2005) and Escherichia coli (40 J/m 2 ; Arrage et al 1993).…”

Section: Cellular Effects Of Uv-c Treatmentmentioning

confidence: 82%

Survival of thermophilic and hyperthermophilic microorganisms after exposure to UV-C, ionizing radiation and desiccation

Beblo

Douki

Schmalz³

et al. 2011

Arch Microbiol

Self Cite

View full text Add to dashboard Cite

In this study, we investigated the ability of several (hyper-) thermophilic Archaea and phylogenetically deep-branching thermophilic Bacteria to survive high fluences of monochromatic UV-C (254 nm) and high doses of ionizing radiation, respectively. Nine out of fourteen tested microorganisms showed a surprisingly high tolerance against ionizing radiation, and two species (Aquifex pyrophilus and Ignicoccus hospitalis) were even able to survive 20 kGy. Therefore, these species had a comparable survivability after exposure to ionizing radiation such as Deinococcus radiodurans. In contrast, there was nearly no difference in survival of the tested strains after exposure to UV-C under anoxic conditions. If the cells had been dried in advance of UV-C irradiation, they were more sensitive to UV-C radiation compared with cells irradiated in liquid suspension; this effect could be reversed by the addition of protective material like sulfidic ores before irradiation. By exposure to UV-C, photoproducts were formed in the DNA of irradiated Archaea and Bacteria. The distribution of the main photoproducts was species specific, but the amount of the photoproducts was only partly dependent on the applied fluence. Overall, our results show that tolerance to radiation seems to be a common phenomenon among thermophilic and hyperthermophilic microorganisms.

show abstract

Section: Cellular Effects Of Uv-c Treatmentmentioning

confidence: 82%

Survival of thermophilic and hyperthermophilic microorganisms after exposure to UV-C, ionizing radiation and desiccation

Beblo

Douki

Schmalz³

et al. 2011

Arch Microbiol

Self Cite

View full text Add to dashboard Cite

show abstract

“…However, percentages vary and some differences are observed, since certain methods seem to over-represent definite groups: Deinococcus-Thermus and Spirochaetes with Qiime and Gammaproteobacteria with MG-RAST. The presence of Deinococcus-Thermus in this environment is expected given the known UV-resistance of members of the Deinococcus genus 39 . However, their relative abundance is difficult to assess precisely because of the different results provided by the methodologies used.…”

Section: Resultsmentioning

confidence: 99%

Arsenic metabolism in high altitude modern stromatolites revealed by metagenomic analysis

Kurth

Amadío

Ordoñez

et al. 2017

Sci Rep

View full text Add to dashboard Cite

Modern stromatolites thrive only in selected locations in the world. Socompa Lake, located in the Andean plateau at 3570 masl, is one of the numerous extreme Andean microbial ecosystems described over recent years. Extreme environmental conditions include hypersalinity, high UV incidence, and high arsenic content, among others. After Socompa’s stromatolite microbial communities were analysed by metagenomic DNA sequencing, taxonomic classification showed dominance of Proteobacteria, Bacteroidetes and Firmicutes, and a remarkably high number of unclassified sequences. A functional analysis indicated that carbon fixation might occur not only by the Calvin-Benson cycle, but also through alternative pathways such as the reverse TCA cycle, and the reductive acetyl-CoA pathway. Deltaproteobacteria were involved both in sulfate reduction and nitrogen fixation. Significant differences were found when comparing the Socompa stromatolite metagenome to the Shark Bay (Australia) smooth mat metagenome: namely, those involving stress related processes, particularly, arsenic resistance. An in-depth analysis revealed a surprisingly diverse metabolism comprising all known types of As resistance and energy generating pathways. While the ars operon was the main mechanism, an important abundance of arsM genes was observed in selected phyla. The data resulting from this work will prove a cornerstone for further studies on this rare microbial community.

show abstract

“…Although resistant to UV-C, D. radiodurans is more sensitive to UV-A (320 to 400 nm), which comprises about 95% of terrestrial UV radiation, than is E. coli (43,69,81,494). For the same inactivation level the amount of BPPs is higher in UV-A-than in UV-C-irradiated D. radiodurans cells (494).…”

Section: Sensitivity Of D Radiodurans To Uv-a Radiation and Singlet mentioning

confidence: 99%

Oxidative Stress Resistance inDeinococcus radiodurans

Slade

Radman

2011

Microbiol Mol Biol Rev

660

639

View full text Add to dashboard Cite

SUMMARY Deinococcus radiodurans is a robust bacterium best known for its capacity to repair massive DNA damage efficiently and accurately. It is extremely resistant to many DNA-damaging agents, including ionizing radiation and UV radiation (100 to 295 nm), desiccation, and mitomycin C, which induce oxidative damage not only to DNA but also to all cellular macromolecules via the production of reactive oxygen species. The extreme resilience of D. radiodurans to oxidative stress is imparted synergistically by an efficient protection of proteins against oxidative stress and an efficient DNA repair mechanism, enhanced by functional redundancies in both systems. D. radiodurans assets for the prevention of and recovery from oxidative stress are extensively reviewed here. Radiation- and desiccation-resistant bacteria such as D. radiodurans have substantially lower protein oxidation levels than do sensitive bacteria but have similar yields of DNA double-strand breaks. These findings challenge the concept of DNA as the primary target of radiation toxicity while advancing protein damage, and the protection of proteins against oxidative damage, as a new paradigm of radiation toxicity and survival. The protection of DNA repair and other proteins against oxidative damage is imparted by enzymatic and nonenzymatic antioxidant defense systems dominated by divalent manganese complexes. Given that oxidative stress caused by the accumulation of reactive oxygen species is associated with aging and cancer, a comprehensive outlook on D. radiodurans strategies of combating oxidative stress may open new avenues for antiaging and anticancer treatments. The study of the antioxidation protection in D. radiodurans is therefore of considerable potential interest for medicine and public health.

show abstract

Roles of PprA, IrrE, and RecA in the resistance of Deinococcus radiodurans to germicidal and environmentally relevant UV radiation

Cited by 34 publications

References 34 publications

Survival of thermophilic and hyperthermophilic microorganisms after exposure to UV-C, ionizing radiation and desiccation

Survival of thermophilic and hyperthermophilic microorganisms after exposure to UV-C, ionizing radiation and desiccation

Arsenic metabolism in high altitude modern stromatolites revealed by metagenomic analysis

Oxidative Stress Resistance inDeinococcus radiodurans

Contact Info

Product

Resources

About