Roles of Sp1 and protein kinase C in regulation of human serum paraoxonase 1 (PON1) gene transcription in HepG2 cells

Osaki, Fumiaki; Ikeda, Yukio; Suehiro, Tadashi; Ota, Kikuko; Tsuzura, Sachie; Arii, Kaoru; Kumon, Yoshitaka; Hashimoto, Kozo

doi:10.1016/j.atherosclerosis.2004.05.029

Cited by 54 publications

(58 citation statements)

References 32 publications

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“…Sp1 is a ubiquitous transcription factor and also reportedly activate PON1 gene transcriptions [23,27]. We also showed that PON1 gene promoter transcription was associated with Sp1 in this study.…”

Section: Role Of P44/42 Map Kinase Signaling Cascade In Sp1 Binding Tsupporting

confidence: 77%

“…Huh7 cells were grown to confluence, harvested, and lysed as described previously [23]. The protein concentration was adjusted (Bio-Rad Protein Assay, Bio-Rad, Hercules, CA).…”

Section: Cell Lysis and Western Blottingmentioning

confidence: 99%

“…The protein concentration was adjusted (Bio-Rad Protein Assay, Bio-Rad, Hercules, CA). Western blotting was performed as described previously [23]. Antibodies for PON1 [8] …”

Section: Cell Lysis and Western Blottingmentioning

confidence: 99%

“…Huh7 cells were grown to confluence and harvested, and the nuclear fraction was isolated and extracted as described previously [16,23]. EMSA was performed as described previously [16,23].…”

Section: Preparation Of Nuclear Extracts and Electrophoretic Mobilitymentioning

confidence: 99%

“…EMSA was performed as described previously [16,23]. The synthetic sense and antisense strands of oligonucleotides (-187/-159) were 5'-GGTGGGGGCTGACCGCAAGCCGCGC-3' and 5'-GGCGCGGCTTGCGGTCAGCCCCCAC-3', respectively.…”

Section: Preparation Of Nuclear Extracts and Electrophoretic Mobilitymentioning

confidence: 99%

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Pitavastatin induces PON1 expression through p44/42 mitogen-activated protein kinase signaling cascade in Huh7 cells

et al. 2009

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Section: Role Of P44/42 Map Kinase Signaling Cascade In Sp1 Binding Tsupporting

confidence: 77%

“…Huh7 cells were grown to confluence, harvested, and lysed as described previously [23]. The protein concentration was adjusted (Bio-Rad Protein Assay, Bio-Rad, Hercules, CA).…”

Section: Cell Lysis and Western Blottingmentioning

confidence: 99%

“…The protein concentration was adjusted (Bio-Rad Protein Assay, Bio-Rad, Hercules, CA). Western blotting was performed as described previously [23]. Antibodies for PON1 [8] …”

Section: Cell Lysis and Western Blottingmentioning

confidence: 99%

“…Huh7 cells were grown to confluence and harvested, and the nuclear fraction was isolated and extracted as described previously [16,23]. EMSA was performed as described previously [16,23].…”

Section: Preparation Of Nuclear Extracts and Electrophoretic Mobilitymentioning

confidence: 99%

Section: Preparation Of Nuclear Extracts and Electrophoretic Mobilitymentioning

confidence: 99%

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Pitavastatin induces PON1 expression through p44/42 mitogen-activated protein kinase signaling cascade in Huh7 cells

et al. 2009

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The Effect ofIn VitroDieldrin Exposure on the Rat Paraoxonase 1 (Pon1) Promoter

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et al. 2014

J Biochem Mol Toxicol

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The legacy organochlorine insecticide, dieldrin, is still found in soil and accumulation in individuals is possible. Paraoxonase 1 hydrolyzes the oxon metabolites of organophosphorus insecticides, as well as other substrates. Putative binding sites for pregnane X receptor (PXR) exist in the paraoxonase promoter, and studies have indicated that dieldrin can activate PXR-regulated gene expression. We examined rat paraoxonase promoter activity in the presence of dieldrin alone or combined with nuclear receptors (NRs). In vitro dieldrin concentrations from 10 to 100 µM significantly increased (p < 0.05) promoter activity in the presence of Pxr or Rxrα alone and when Pxr plus Rxrα were on the same vector, indicating that dieldrin can increase paraoxonase promoter activity in the presence of NRs. To our knowledge, this is the first report of dieldrin increasing paraoxonase promoter activity. Since many organochlorine insecticides are in the same chemical class as dieldrin, these results could be typical of other bioaccumulative persistent pollutants.

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A short peptide is a protein kinase C (PKC) α‐specific substrate

et al. 2008

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The purpose of this study was to find protein kinase C (PKC) isozyme-specific peptides. A peptide library containing 1772 sequences was designed using Scansite and screened by MALDI-TOF MS and kinase activity assays for PKC isozyme-specificity. A peptide (Alphatomega; H-FKKQGSFAKKK-NH(2)) with high specificity for PKC alpha relative to other isozymes was identified. The peptide was phosphorylated to a greater extent by tissue lysates from B16 melanoma, HepG2, and human breast cancer, which had higher levels of activated PKC alpha, when compared to normal skin, liver, and human breast tissue lysates, respectively. Moreover, addition of Ro-31-7549, an inhibitor with great specificity for PKC alpha, to the phosphorylation reaction caused a dose-dependent reduction in phosphorylation, but no inhibition was identified with the addition of rottlerin and H-89. These results show that this peptide has great potential as a PKC alpha-specific substrate.

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Roles of Sp1 and protein kinase C in regulation of human serum paraoxonase 1 (PON1) gene transcription in HepG2 cells

Cited by 54 publications

References 32 publications

Pitavastatin induces PON1 expression through p44/42 mitogen-activated protein kinase signaling cascade in Huh7 cells

Pitavastatin induces PON1 expression through p44/42 mitogen-activated protein kinase signaling cascade in Huh7 cells

The Effect ofIn VitroDieldrin Exposure on the Rat Paraoxonase 1 (Pon1) Promoter

A short peptide is a protein kinase C (PKC) α‐specific substrate

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