Roles of Surfactants in Oriented Immobilization of Cellulase on Nanocarriers and Multiphase Hydrolysis System

Wang, Zhiquan; Fan, Chunzhen; Zheng, Xiangyong; Jin, Zhan; Bei, Ke; Zhao, Min; Kong, Hainan

doi:10.3389/fchem.2022.884398

Cited by 3 publications

(2 citation statements)

References 146 publications

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“…Sahin [16], for instance, used 3-(3,4-dihydroxyphenyl) propionic acid-functionalized TiO 2 nanoparticles as matrices for HRP immobilization. Given their abundant availability and polymeric nature, the use of non-metallic nanoparticles, including biodegradable chitosan or thermally stable silica, has also gained popularity [17]. Melo et al [18] combined chitosan nanoparticles with polyethylene glycol (PEG) for horseradish peroxidase immobilization.…”

Section: Introductionmentioning

confidence: 99%

Support Materials of Organic and Inorganic Origin as Platforms for Horseradish Peroxidase Immobilization: Comparison Study for High Stability and Activity Recovery

Bilal,

Degorska,

Szada

et al. 2024

Molecules

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In the presented study, a variety of hybrid and single nanomaterials of various origins were tested as novel platforms for horseradish peroxidase immobilization. A thorough characterization was performed to establish the suitability of the support materials for immobilization, as well as the activity and stability retention of the biocatalysts, which were analyzed and discussed. The physicochemical characterization of the obtained systems proved successful enzyme deposition on all the presented materials. The immobilization of horseradish peroxidase on all the tested supports occurred with an efficiency above 70%. However, for multi-walled carbon nanotubes and hybrids made of chitosan, magnetic nanoparticles, and selenium ions, it reached up to 90%. For these materials, the immobilization yield exceeded 80%, resulting in high amounts of immobilized enzymes. The produced system showed the same optimal pH and temperature conditions as free enzymes; however, over a wider range of conditions, the immobilized enzymes showed activity of over 50%. Finally, a reusability study and storage stability tests showed that horseradish peroxidase immobilized on a hybrid made of chitosan, magnetic nanoparticles, and selenium ions retained around 80% of its initial activity after 10 repeated catalytic cycles and after 20 days of storage. Of all the tested materials, the most favorable for immobilization was the above-mentioned chitosan-based hybrid material. The selenium additive present in the discussed material gives it supplementary properties that increase the immobilization yield of the enzyme and improve enzyme stability. The obtained results confirm the applicability of these nanomaterials as useful platforms for enzyme immobilization in the contemplation of the structural stability of an enzyme and the high catalytic activity of fabricated biocatalysts.

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Section: Introductionmentioning

confidence: 99%

Support Materials of Organic and Inorganic Origin as Platforms for Horseradish Peroxidase Immobilization: Comparison Study for High Stability and Activity Recovery

Bilal,

Degorska,

Szada

et al. 2024

Molecules

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“…Because of the growing number of publications on cellulase immobilized on nanostructured supports, in this review we will discuss the above developments using the literature mainly published from the beginning of 2017 through August of 2022. It is important to mention preceding recent reviews on the topics related to cellulase immobilization on nanostructured supports [ 9 , 25 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 ] as they created the groundwork for our analysis.…”

Section: Introductionmentioning

confidence: 99%

Cellulase Immobilization on Nanostructured Supports for Biomass Waste Processing

2022

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Nanobiocatalysts, i.e., enzymes immobilized on nanostructured supports, received considerable attention because they are potential remedies to overcome shortcomings of traditional biocatalysts, such as low efficiency of mass transfer, instability during catalytic reactions, and possible deactivation. In this short review, we will analyze major aspects of immobilization of cellulase—an enzyme for cellulosic biomass waste processing—on nanostructured supports. Such supports provide high surface areas, increased enzyme loading, and a beneficial environment to enhance cellulase performance and its stability, leading to nanobiocatalysts for obtaining biofuels and value-added chemicals. Here, we will discuss such nanostructured supports as carbon nanotubes, polymer nanoparticles (NPs), nanohydrogels, nanofibers, silica NPs, hierarchical porous materials, magnetic NPs and their nanohybrids, based on publications of the last five years. The use of magnetic NPs is especially favorable due to easy separation and the nanobiocatalyst recovery for a repeated use. This review will discuss methods for cellulase immobilization, morphology of nanostructured supports, multienzyme systems as well as factors influencing the enzyme activity to achieve the highest conversion of cellulosic biowaste into fermentable sugars. We believe this review will allow for an enhanced understanding of such nanobiocatalysts and processes, allowing for the best solutions to major problems of sustainable biorefinery.

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Effects of Sisal Saponins on Enzymatic Hydrolysis and Fermentation of Lignocellulosic Biomass

da Costa,

dos Santos,

de Freitas

et al. 2024

Bioenerg. Res.

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Roles of Surfactants in Oriented Immobilization of Cellulase on Nanocarriers and Multiphase Hydrolysis System

Cited by 3 publications

References 146 publications

Support Materials of Organic and Inorganic Origin as Platforms for Horseradish Peroxidase Immobilization: Comparison Study for High Stability and Activity Recovery

Support Materials of Organic and Inorganic Origin as Platforms for Horseradish Peroxidase Immobilization: Comparison Study for High Stability and Activity Recovery

Cellulase Immobilization on Nanostructured Supports for Biomass Waste Processing

Effects of Sisal Saponins on Enzymatic Hydrolysis and Fermentation of Lignocellulosic Biomass

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