Roles of the<i>C. elegans</i>cyclophilin-like protein MOG-6 in MEP-1 binding and germline fates

Belfiore, Marco; Pugnale, Paolo; Saudan, Zarifja; Puoti, Alessandro

doi:10.1242/dev.01154

Cited by 48 publications

(65 citation statements)

References 39 publications

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“…Eckmann, in prep.). It is also consistent with the fact that gld-3(RNAi) knockdowns result in tumorogenic germlines that fail to progress through meiosis, suggesting that GLD-3 does have a more central role in promoting the meiotic cell fate (Belfiore et al 2004). Whether both PAP complexes form on a single GLD-3 molecule, and thus on the same mRNA, is unclear, and is not a necessity for their communal function.…”

Section: Gld-4 Is a Regulatory Pap And P Granule Componentsupporting

confidence: 69%

Two conserved regulatory cytoplasmic poly(A) polymerases, GLD-4 and GLD-2, regulate meiotic progression in C. elegans

Schmid¹,

Küchler²,

Eckmann³

2009

Genes Dev.

105

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Translational regulation is heavily employed during developmental processes to control the timely accumulation of proteins independently of gene transcription. In particular, mRNA poly(A) tail metabolism in the cytoplasm is a key determinant for balancing an mRNA's translational output and its decay rate. Noncanonical poly(A) polymerases (PAPs), such as germline development defective-2 (GLD-2), can mediate poly(A) tail extension. Little is known about the regulation and functional complexity of cytoplasmic PAPs. Here we report the discovery of Caenorhabditis elegans GLD-4, a cytoplasmic PAP present in P granules that is orthologous to Trf4/5p from budding yeast. GLD-4 enzymatic activity is enhanced by its interaction with GLS-1, a protein associated with the RNA-binding protein GLD-3. GLD-4 is predominantly expressed in germ cells, and its activity is essential for early meiotic progression of male and female gametes in the absence of GLD-2. For commitment into female meiosis, both PAPs converge on at least one common target mRNA—i.e., gld-1 mRNA—and, as a consequence, counteract the repressive action of two PUF proteins and the putative deadenylase CCR-4. Together our findings suggest that two different cytoplasmic PAPs stabilize and translationally activate several meiotic mRNAs to provide a strong fail-safe mechanism for early meiotic progression.

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Section: Gld-4 Is a Regulatory Pap And P Granule Componentsupporting

confidence: 69%

Two conserved regulatory cytoplasmic poly(A) polymerases, GLD-4 and GLD-2, regulate meiotic progression in C. elegans

Schmid¹,

Küchler²,

Eckmann³

2009

Genes Dev.

105

View full text Add to dashboard Cite

show abstract

“…The canonical cell cycle machinery controls GSC mitotic divisions, as expected; in addition, many RNA regulatory proteins affect the mitosis/meiosis balance, underscoring the importance of posttranscriptional controls of C. elegans germ cell cycles (e.g., Belfiore et al 2004;Ciosk et al 2004;Eckmann et al 2004;Maine et al 2004;Hansen et al 2005;Hasegawa et al 2006;Ariz et al 2009;Kerins et al 2010). In addition, micro RNAs have been implicated in GSC proliferation controls (Cox et al 1998;Smardon et al 2000).…”

Section: Shifting the Balance Between Mitotic And Meiotic Modes Of Thmentioning

confidence: 59%

Molecular Regulation of the Mitosis/Meiosis Decision in Multicellular Organisms

Kimble

2011

Cold Spring Harbor Perspectives in Biology

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“…We reasoned that if MOG proteins down-regulated fem-3 RNA, both proteins should not be found within the same germ cells. MOG proteins are present in the nuclei of many germ cells, including oocytes (Belfiore et al 2004;Kasturi et al 2010;Zanetti et al 2011). We show that FEM-3 labels the spermatogenic lineage.…”

Section: Fem-3 and Mog Expression Are Mutually Exclusivementioning

confidence: 72%

The sperm-oocyte switch in the C. elegans hermaphrodite is controlled through steady-state levels of the fem-3 mRNA

Zanetti¹,

Grinschgl²,

Meola³

et al. 2012

RNA

Self Cite

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Post-transcriptional control regulates many aspects of germline development in the Caenorhabditis elegans hermaphrodite. This nematode switches from spermatogenesis to oogenesis and is, therefore, capable of self-fertilization. This sperm-oocyte switch requires 39 UTR-mediated repression of the fem-3 mRNA. Loss of fem-3 repression results in continuous spermatogenesis in hermaphrodites. Although several factors regulating fem-3 have been identified, little is known about the mechanisms that control fem-3. Here, we investigate the steady-state levels of the fem-3 transcript and the expression pattern of its protein product. We show that FEM-3 is exclusively present in germ cells that are committed to spermatogenesis. We found that in fem-3(gf)/+ heterozygotes, mutant fem-3 gain-of-function transcripts are more abundant than their wild-type counterpart. Furthermore, we show that the penetrance of the fem-3(gf) allele correlates with inefficient FBF binding and extended poly(A) tail size of fem-3 mRNAs. Finally, we show that wild-type and gain-of-function mutated fem-3 mRNAs associate equally well with polyribosomes. We propose that the fem-3 mRNA is regulated through stabilization rather than through translatability.

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Roles of theC. eleganscyclophilin-like protein MOG-6 in MEP-1 binding and germline fates

Cited by 48 publications

References 39 publications

Two conserved regulatory cytoplasmic poly(A) polymerases, GLD-4 and GLD-2, regulate meiotic progression in C. elegans

Two conserved regulatory cytoplasmic poly(A) polymerases, GLD-4 and GLD-2, regulate meiotic progression in C. elegans

Molecular Regulation of the Mitosis/Meiosis Decision in Multicellular Organisms

The sperm-oocyte switch in the C. elegans hermaphrodite is controlled through steady-state levels of the fem-3 mRNA

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